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Rabbit Recombinant Monoclonal RIF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.

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Images

Western blot - Anti-Rif1 antibody [EPR27131-83] (AB316748), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody [EPR27131-83] (AB316748), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (AB316748), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (AB316748), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (AB316748), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs (PubMed:15342490, PubMed:28241136). In response to DNA damage, interacts with ATM-phosphorylated TP53BP1 (PubMed:23333306, PubMed:28241136). Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs (PubMed:28241136). Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs (PubMed:23333306). In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase (PubMed:23333306). Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (By similarity). Promotes NHEJ of dysfunctional telomeres (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal RIF1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27131-83

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Rif1 also known as Rap1-interacting factor 1 is a protein that aids in DNA end protection and repair processes. The protein has a molecular mass of approximately 240 kDa and is predominantly expressed in the nucleus of eukaryotic cells. Rif1 interacts directly with telomeric DNA and regulates DNA-double strand break (DSB) repair. Its activity ensures genomic integrity by coordinating access to damaged DNA ends which is essential in the context of maintaining telomere length and chromosomal stability.

Biological function summary

Rif1 plays a central role in the DNA damage response and DNA replication. It operates as part of the shieldin complex which participates in non-homologous end joining (NHEJ) by protecting DNA ends from extensive resection. By doing so Rif1 directs the repair pathway choice towards NHEJ over homologous recombination enabling cell cycle progression in conditions of DNA stress. Its function is particularly significant during the S and G1 phases of the cell cycle during which Rif1 modulates replication timing and forks stalling recovery.

Pathways

Several critical cellular processes involve Rif1 particularly DNA damage repair and cell cycle regulation. Within these pathways Rif1 interacts with proteins such as 53BP1 which is instrumental in controlling pathway selectivity during DNA repair and it influences origin firing through its interplay with replication timing guards. Additionally Rif1 works in conjunction with ATM and ATR kinases which are key regulators in the phosphorylation cascade triggered by DNA damage. These interactions underpin Rif1’s pivotal role in cell cycle checkpoints and repair signaling pathways.

Associated diseases and disorders

Aberrations in Rif1 function have links to cancer and premature aging diseases. Altered Rif1 expression or malfunction can contribute to tumorigenesis especially in cancers where DNA repair pathways are disrupted. This is due in part to its relationship with 53BP1 playing roles in tumor suppression pathways by maintaining genomic stability. Furthermore mutations affecting Rif1 are associated with conditions like Hutchinson-Gilford Progeria Syndrome. This connection to lamin A/C another protein involved in nuclear stability highlights its broader impact on maintaining cellular homeostasis and genome integrity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-Rif1 antibody [EPR27131-83] (ab316748), expandable thumbnail

    Western blot - Anti-Rif1 antibody [EPR27131-83] (ab316748)

    The expression profile/ molecular weight observed isconsistent with what has been described in the literature (PMID: 34736895).

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    The identity of the band below 100 kDa is unknown.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-Rif1 antibody [EPR27131-83] (ab316748) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 40 µg

    Lane 2: HeLa transfected with siRNA specifically targeting Rif1 whole cell lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 110-310 kDa, 124 kDa

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody [EPR27131-83] (ab316748), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Rif1 antibody [EPR27131-83] (ab316748)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling Rif1 with ab316748 at 1/50 (10.4 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing nuclear staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (ab316748), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (ab316748)

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Rif1 with ab316748 at 1/100 (5.2 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining in human endometrium (PMID: 15583028).

    The section was incubated with ab316748 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (ab316748), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (ab316748)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Rif1 with ab316748 at 1/100 (5.2 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining in human lung (PMID: 30237512).

    The section was incubated with ab316748 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (ab316748), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (ab316748)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell lung carcinoma  tissue labeling Rif1 with ab316748 at 1/100 (5.2 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human squamous cell lung carcinoma tissue (PMID: 30237512).

    The section was incubated with ab316748 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (ab316748), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rif1 antibody [EPR27131-83] (ab316748)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Rif1 with ab316748 at 1/100 (5.2 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human testis (PMID: 15583028).

    The section was incubated with ab316748 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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