AB325011

Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11]

Recombinant
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Mouse Recombinant Monoclonal NCAP antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Rift valley fever virus (STRAIN ZH-548 M12), Rift valley fever virus (STRAIN ZH-548 M12) samples.

View Alternative Names

Nucleoprotein, Nucleocapsid protein, Protein N

2 Images

Indirect ELISA - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (AB325011)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (AB325011)

Indirect ELISA analysis of ab325011 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution dilution.

Antigen : Rift Valley Fever Virus Nucleoprotein.

Antigen concentration : 1000 ng/ml

Supplier Data

Western blot - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (AB325011)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (ab325011) at 1/1000 dilution

Lane 1:

293T cells transfected with Rift valley fever virus (RVFV) Nucleoprotein (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 1 µg

Lane 2:

293T cells transfected with Rift valley fever virus (RVFV) Envelopment polyprotein (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with Rift valley fever virus (RVFV) RNA-directed RNA polymerase L (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with Rift valley fever virus (RVFV) Non-structural protein S (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 5 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 28-243 kDa,36 kDa

false

Exposure time: 180s

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AA11

Isotype

IgG2a

Carrier free

Reacts with

Rift valley fever virus (STRAIN ZH-548 M12)

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rift valley fever virus (STRAIN ZH-548 M12)": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "31.25 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Rift valley fever virus (STRAIN ZH-548 M12)": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Encapsidates the genomic RNA, protecting it from nucleases (By similarity). Displays high affinity for single-stranded nucleic acid (By similarity). The encapsidated genomic RNA is termed the nucleocapsid (NC) (By similarity). The ribonucleoprotein has a non-helical structure (By similarity). Serves as template for viral transcription and replication (PubMed : 7769655). After replication, the nucleocapsid is recruited to the host Golgi apparatus by glycoprotein Gn for packaging into virus particles (PubMed : 21445316).

See full target information N

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com