AB238254

Anti-RIG-I/DDX58 antibody

Name: Anti-RIG-I/DDX58 antibody (ab238254) | Abcam
Brand: Abcam Limited
SKU: AB238254
Price: 525 USD
Availability: InStock

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(4 Publications)

Goat Polyclonal RIG-I/DDX58 antibody. Suitable for IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human RIGI aa 900 to C-terminus.

View Alternative Names

DDX58, RIGI, Antiviral innate immune response receptor RIG-I, ATP-dependent RNA helicase DDX58, DEAD box protein 58, RIG-I-like receptor 1, RNA sensor RIG-I, Retinoic acid-inducible gene 1 protein, Retinoic acid-inducible gene I protein, RLR-1, RIG-1, RIG-I

3 Images

Flow Cytometry (Intracellular) - Anti-RIG-I/DDX58 antibody (AB238254)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RIG-I/DDX58 antibody (AB238254)

Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton-X-100 permeabilized U937 (human histiocytic lymphoma cell line) cells labeling RIG-I/DDX58 with ab238254 at 10 μg/ml (blue) compared with unimmunized Goat IgG (black). An AlexaFluor^®488 secondary antibody was used at 2 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody (AB238254)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody (AB238254)

Paraformaldehyde-fixed, 0.15% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RIG-I/DDX58 with ab238254 at 10 μg/ml in ICC/IF, followed by AlexaFluor^® 488 conjugated secondary antibody at 2 μg/ml (green). The nuclear counter stain is DAPI (blue).

-ve control : Unimmunized Goat IgG at 10 μg/ml, followed by AlexaFluor^® 488 conjugated secondary antibody at 2 μg/ml.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody (AB238254)

Paraformaldehyde-fixed, 0.15% Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling RIG-I/DDX58 with ab238254 at 10 μg/ml in ICC/IF, followed by AlexaFluor^® 488 conjugated secondary antibody at 2 μg/ml (green). The nuclear counter stain is DAPI (blue).

-ve control : Unimmunized Goat IgG at 10 μg/ml, followed by AlexaFluor^® 488 conjugated secondary antibody at 2 μg/ml.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human RIGI aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95786

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.75 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIG-I also known as DDX58 is an important protein with mass of approximately 102 kDa. This protein acts as a cytosolic sensor for viral double-stranded RNA playing an essential role in the detection of viral infections. It is expressed in many cell types including immune and epithelial cells. RIG-I consists of two N-terminal caspase activation and recruitment domains (CARDs) a DExD/H box helicase domain and a C-terminal regulatory domain. These domains enable RIG-I to recognize and bind viral RNA initiating downstream signaling for immune responses.

Biological function summary

RIG-I contributes significantly to the innate immune response. It acts to sense viral RNA and triggers the production of type I interferons and other pro-inflammatory cytokines. This protein functions as part of a complex that includes MAVS (mitochondrial antiviral signaling protein) and other signaling adapters. Upon activation RIG-I undergoes a conformational change leading to the exposure of its CARDs which interact with CARDs of MAVS facilitating downstream signaling to induce an antiviral state in host cells.

Pathways

RIG-I plays a central role in the RNA sensing pathway critical for antiviral immunity. This pathway involves several steps beginning with the recognition of viral RNA leading to the activation of interferon regulatory factors like IRF3 and IRF7 as well as nuclear factor kappa B (NF-κB). These factors then promote the expression of interferon-stimulated genes (ISGs). RIG-I also relates closely to the Jak-STAT signaling pathway which is activated by interferons and enhances the transcription of ISGs further amplifying the antiviral response.

RIG-I is often associated with viral infections such as hepatitis C and influenza. By detecting viral RNA RIG-I activates immune responses that help to control these infections. However dysfunction or aberrations in RIG-I signaling can lead to autoimmune disorders such as Aicardi-Goutières syndrome where there is an inappropriate response to self nucleic acids. In these disease contexts RIG-I interacts with MAVS and indirectly with proteins involved in regulating immune responses such as STING which plays a role in the innate immune response against DNA viruses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Innate immune receptor that senses cytoplasmic viral nucleic acids and activates a downstream signaling cascade leading to the production of type I interferons and pro-inflammatory cytokines (PubMed : 15208624, PubMed : 15708988, PubMed : 16125763, PubMed : 16127453, PubMed : 16153868, PubMed : 17190814, PubMed : 18636086, PubMed : 19122199, PubMed : 19211564, PubMed : 24366338, PubMed : 28469175, PubMed : 29117565, PubMed : 31006531, PubMed : 34935440, PubMed : 35263596, PubMed : 36793726). Forms a ribonucleoprotein complex with viral RNAs on which it homooligomerizes to form filaments (PubMed : 15208624, PubMed : 15708988). The homooligomerization allows the recruitment of RNF135 an E3 ubiquitin-protein ligase that activates and amplifies the RIG-I-mediated antiviral signaling in an RNA length-dependent manner through ubiquitination-dependent and -independent mechanisms (PubMed : 28469175, PubMed : 31006531). Upon activation, associates with mitochondria antiviral signaling protein (MAVS/IPS1) that activates the IKK-related kinases TBK1 and IKBKE which in turn phosphorylate the interferon regulatory factors IRF3 and IRF7, activating transcription of antiviral immunological genes including the IFN-alpha and IFN-beta interferons (PubMed : 28469175, PubMed : 31006531). Ligands include 5'-triphosphorylated ssRNAs and dsRNAs but also short dsRNAs (<1 kb in length) (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity (PubMed : 15208624, PubMed : 15708988, PubMed : 19576794, PubMed : 19609254, PubMed : 21742966). Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae : Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae : vesicular stomatitis virus (VSV), Orthomyxoviridae : influenza A and B virus, Flaviviridae : Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV) (PubMed : 21616437, PubMed : 21884169). It also detects rotaviruses and reoviruses (PubMed : 21616437, PubMed : 21884169). Detects and binds to SARS-CoV-2 RNAs which is inhibited by m6A RNA modifications (Ref.74). Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV) (PubMed : 19631370). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration.

See full target information RIGI

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 34:311-325 PubMed38274293

2024

Nanoparticle-encapsulated retinoic acid for the modulation of bone marrow hematopoietic stem cell niche.

Applications

Unspecified application

Species

Unspecified reactive species

Emanuel Quartin,Susana Rosa,Sara Gonzalez-Anton,Laura Mosteo Lopez,Vitor Francisco,Delfim Duarte,Cristina Lo Celso,Ricardo Pires das Neves,Lino Ferreira

Oncogenesis 11:40 PubMed35858925

2022

The E3 ubiquitin ligase MG53 inhibits hepatocellular carcinoma by targeting RAC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomin Ma,Xiaoxiao Ma,Lihui Zhu,Yunxue Zhao,Mengmeng Chen,Tao Li,Yueke Lin,Dapeng Ma,Caiyu Sun,Lihui Han

Molecular cancer 21:97 PubMed35395767

2022

The m6A demethylase ALKBH5 promotes tumor progression by inhibiting RIG-I expression and interferon alpha production through the IKKε/TBK1/IRF3 pathway in head and neck squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang Jin,Mingyu Li,Hanyue Chang,Ruijie Wang,Zhiyuan Zhang,Jianjun Zhang,Yue He,Hailong Ma

Experimental dermatology 31:1165-1176 PubMed35332586

2022

Ultraviolet light induces HERV expression to activate RIG-I signalling pathway in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Min,Meiling Zheng,Yaqin Yu,Jiali Wu,Qiqi Kuang,Zhi Hu,Lianlian Ouyang,Shuang Lu,Ming Zhao

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Product promise

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Anti-RIG-I/DDX58 antibody

Flow Cytometry (Intracellular) - Anti-RIG-I/DDX58 antibody (AB238254)

Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody (AB238254)

Immunocytochemistry/ Immunofluorescence - Anti-RIG-I/DDX58 antibody (AB238254)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise