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Rabbit Recombinant Monoclonal RIL antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-RIL antibody [EPR16199] (AB197026), expandable thumbnail
  • Western blot - Anti-RIL antibody [EPR16199] (AB197026), expandable thumbnail
  • Western blot - Anti-RIL antibody [EPR16199] (AB197026), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Isoform 1. Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle (PubMed:19307596). Involved in reorganization of the actin cytoskeleton (PubMed:21636573). In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane. Links endosomal surface-internalized GRIA1-containing AMPA receptors to the alpha-actinin/actin cytoskeleton. Increases AMPA receptor-mediated excitatory postsynaptic currents in neurons (By similarity). Isoform 2. Involved in reorganization of the actin cytoskeleton and in regulation of cell migration. In response to oxidative stress, binds to NQO1, which stabilizes it and protects it from ubiquitin-independent degradation by the core 20S proteasome. Stabilized protein is able to heterodimerize with isoform 1 changing the subcellular location of it from cytoskeleton and nuclei to cytosol, leading to loss of isoforms 1 ability to induce formation of actin stress fibers. Counteracts the effects produced by isoform 1 on organization of actin cytoskeleton and cell motility to fine-tune actin cytoskeleton rearrangement and to attenuate cell migration.

Alternative names

RIL, PDLIM4, PDZ and LIM domain protein 4, LIM protein RIL, Reversion-induced LIM protein

Recommended products

Rabbit Recombinant Monoclonal RIL antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16199
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RIL also known as PDZ and LIM domain protein 4 (PDLIM4) is a cytoskeletal protein playing a role in linking actin filaments and signaling pathways. It has a mass of approximately 39 kDa. RIL expresses in various tissues including the heart skeletal muscle and the brain. Through its PDZ domain RIL interacts with other proteins influencing structural and signaling functions.

Biological function summary

RIL has a role in maintaining cellular structure and dynamics. It participates in cytoskeletal organization helping stabilize actin filaments within cells. RIL associates with other LIM domain proteins to form complexes that integrate signals directed to the cytoskeleton contributing to cellular integrity and response to environmental cues. It also interacts with alpha-actinin and other cytoskeletal-associated proteins supporting cellular morphology and motility.

Pathways

The protein supports cellular architecture and signaling. It plays roles in the actin cytoskeleton regulatory pathway and the focal adhesion pathway. RIL works alongside proteins such as alpha-actinin and integrins to ensure proper signal transduction and cellular structural organization. Its interaction within these pathways helps in transmitting mechanical and chemical signals essential for cellular responses and adaptations.

Associated diseases and disorders

RIL links to the development of certain cancers and cardiac diseases. Alterations in RIL expression may influence tumor progression and metastasis due to its interactions with cytoskeletal elements. In cardiac disorders RIL's connection with proteins like integrins could lead to abnormal heart muscle function contributing to conditions such as cardiomyopathy. Increased or decreased RIL levels impact cellular signaling and structural stability underpinning its role in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-RIL antibody [EPR16199] (ab197026), expandable thumbnail

    Western blot - Anti-RIL antibody [EPR16199] (ab197026)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RIL antibody [EPR16199] (ab197026) at 1/1000 dilution

    All lanes: PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

  • Western blot - Anti-RIL antibody [EPR16199] (ab197026), expandable thumbnail

    Western blot - Anti-RIL antibody [EPR16199] (ab197026)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RIL antibody [EPR16199] (ab197026) at 1/1000 dilution

    All lanes: A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

  • Western blot - Anti-RIL antibody [EPR16199] (ab197026), expandable thumbnail

    Western blot - Anti-RIL antibody [EPR16199] (ab197026)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-RIL antibody [EPR16199] (ab197026) at 1/10000 dilution

    All lanes: SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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