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AB251233

Anti-RIL antibody [EPR16199] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RIL antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RIL, PDLIM4, PDZ and LIM domain protein 4, LIM protein RIL, Reversion-induced LIM protein

3 Images
Western blot - Anti-RIL antibody [EPR16199] - BSA and Azide free (AB251233)
  • WB

Supplier Data

Western blot - Anti-RIL antibody [EPR16199] - BSA and Azide free (AB251233)

This data was developed using ab197026, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RIL antibody [EPR16199] (<a href='/en-us/products/primary-antibodies/ril-antibody-epr16199-ab197026'>ab197026</a>) at 1/10000 dilution

All lanes:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Western blot - Anti-RIL antibody [EPR16199] - BSA and Azide free (AB251233)
  • WB

Supplier Data

Western blot - Anti-RIL antibody [EPR16199] - BSA and Azide free (AB251233)

This data was developed using ab197026, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RIL antibody [EPR16199] (<a href='/en-us/products/primary-antibodies/ril-antibody-epr16199-ab197026'>ab197026</a>) at 1/1000 dilution

All lanes:

PC-3 (Human prostate cancer cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Western blot - Anti-RIL antibody [EPR16199] - BSA and Azide free (AB251233)
  • WB

Supplier Data

Western blot - Anti-RIL antibody [EPR16199] - BSA and Azide free (AB251233)

This data was developed using ab197026, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RIL antibody [EPR16199] (<a href='/en-us/products/primary-antibodies/ril-antibody-epr16199-ab197026'>ab197026</a>) at 1/1000 dilution

All lanes:

A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16199

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251233 is the carrier-free version of ab197026.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIL also known as PDZ and LIM domain protein 4 (PDLIM4) is a cytoskeletal protein playing a role in linking actin filaments and signaling pathways. It has a mass of approximately 39 kDa. RIL expresses in various tissues including the heart skeletal muscle and the brain. Through its PDZ domain RIL interacts with other proteins influencing structural and signaling functions.
Biological function summary

RIL has a role in maintaining cellular structure and dynamics. It participates in cytoskeletal organization helping stabilize actin filaments within cells. RIL associates with other LIM domain proteins to form complexes that integrate signals directed to the cytoskeleton contributing to cellular integrity and response to environmental cues. It also interacts with alpha-actinin and other cytoskeletal-associated proteins supporting cellular morphology and motility.

Pathways

The protein supports cellular architecture and signaling. It plays roles in the actin cytoskeleton regulatory pathway and the focal adhesion pathway. RIL works alongside proteins such as alpha-actinin and integrins to ensure proper signal transduction and cellular structural organization. Its interaction within these pathways helps in transmitting mechanical and chemical signals essential for cellular responses and adaptations.

RIL links to the development of certain cancers and cardiac diseases. Alterations in RIL expression may influence tumor progression and metastasis due to its interactions with cytoskeletal elements. In cardiac disorders RIL's connection with proteins like integrins could lead to abnormal heart muscle function contributing to conditions such as cardiomyopathy. Increased or decreased RIL levels impact cellular signaling and structural stability underpinning its role in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle (PubMed : 19307596). Involved in reorganization of the actin cytoskeleton (PubMed : 21636573). In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane. Links endosomal surface-internalized GRIA1-containing AMPA receptors to the alpha-actinin/actin cytoskeleton. Increases AMPA receptor-mediated excitatory postsynaptic currents in neurons (By similarity).. Isoform 2. Involved in reorganization of the actin cytoskeleton and in regulation of cell migration. In response to oxidative stress, binds to NQO1, which stabilizes it and protects it from ubiquitin-independent degradation by the core 20S proteasome. Stabilized protein is able to heterodimerize with isoform 1 changing the subcellular location of it from cytoskeleton and nuclei to cytosol, leading to loss of isoforms 1 ability to induce formation of actin stress fibers. Counteracts the effects produced by isoform 1 on organization of actin cytoskeleton and cell motility to fine-tune actin cytoskeleton rearrangement and to attenuate cell migration.
See full target information PDLIM4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:3361-3370 PubMed33682317

2021

TNFAIP8 regulates gastric cancer growth via mTOR-Akt-ULK1 pathway and autophagy signals.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Chen,Jianguo Zhang,Chenyang Dong,Dongsheng Li,Yuehan Yin,Wenhai Yu,Yuezhi Chen
View all publications
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Product promise

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