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AB140188

Anti-RILP antibody

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(9 Publications)

Rabbit Polyclonal RILP antibody. Suitable for WB and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human RILP.

View Alternative Names

PP10141, RILP, Rab-interacting lysosomal protein

2 Images
Western blot - Anti-RILP antibody (AB140188)
  • WB

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Western blot - Anti-RILP antibody (AB140188)

All lanes:

Western blot - Anti-RILP antibody (ab140188) at 1 µg/mL

Lane 1:

A20 cell lysate at 15 µg

Lane 2:

A20 cell lysate at 15 µg with blocking peptide

Predicted band size: 44 kDa

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Western blot - Anti-RILP antibody (AB140188)
  • WB

CiteAb

Western blot - Anti-RILP antibody (AB140188)

RILP western blot using anti-RILP antibody ab140188. Publication image and figure legend from Zhao, Y., Long, Z., et al., 2020, Front Aging Neurosci, PubMed 32210783.

ab140188 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab140188 please see the product overview.

DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target of rapamycin (mTOR), ULK1, p-GSK3β (Ser9), GSK3β in extracts of whole brain tissue were detected by WB. Representative Western blots are presented in panels (A,C,E,G), and quantification of the results is shown as the mean values ± SD in panels (B,D,F,H). *p < 0.05, **p < 0.01, and ***p < 0.001 between two groups. One-way analysis of variance/Newman–Keuls test was performed for all WB data.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RILP. The exact immunogen used to generate this antibody is proprietary information.

Q96NA2

Specificity

At least two isoforms of RILP are known to exist; ab140188 will only detect the longer isoform.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RILP known as Rab-interacting lysosomal protein is a critical component in the regulation of membrane trafficking. The protein weighs approximately 45 kDa. RILP assists in tethering and fusion processes essential for vesicle transport acting as an effector for the small GTPase Rab7. It is mainly expressed in the lysosomal compartments of cells. By binding to Rab7 RILP influences endocytic trafficking by coordinating microtubule-based movement of endosomes and lysosomes.
Biological function summary

RILP collaborates within multiprotein complexes influencing lysosomal positioning and maturation. It mediates recruitment of dynein-dynactin motor complexes allowing effective transport of lysosomes along microtubules. Furthermore RILP's activity impacts the degradation pathway assisting in the clearance of cellular debris and turnover of cellular components. It interacts with various cellular structures to maintain the balance between movement and stability of lysosomes which is essential for cellular homeostasis.

Pathways

Researchers place RILP within significant cellular processes like lysosome positioning and autophagic pathways. Its role is important in the endocytic pathway where it acts with proteins like Rab7 and the retromer complex. RILP governs endosome-to-lysosome trafficking impacting cargo sorting and turnover. It shares functional interactions with proteins involved in autophagy influencing the delivery of cellular components for degradation within lysosomes.

RILP notably connects to neurodegenerative diseases and cancers. Altered RILP function can disrupt lysosome positioning and trafficking leading to disorders like Niemann-Pick disease type C. In cancer RILP's interaction with key signaling proteins contributes to tumor progression and metastasis. The protein's regulatory effects on lysosomal pathways intersect with various cellular processes including those involving proteins such as Rab7 that when dysregulated result in pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Rab effector playing a role in late endocytic transport to degradative compartments (PubMed : 11179213, PubMed : 11696325, PubMed : 12944476, PubMed : 14668488, PubMed : 27113757). Involved in the regulation of lysosomal morphology and distribution (PubMed : 14668488, PubMed : 27113757). Induces recruitment of dynein-dynactin motor complexes to Rab7A-containing late endosome and lysosome compartments (PubMed : 11179213, PubMed : 11696325). Promotes centripetal migration of phagosomes and the fusion of phagosomes with the late endosomes and lysosomes (PubMed : 12944476).
See full target information RILP
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed39859265

2025

The Type III Intermediate Filament Protein Peripherin Regulates Lysosomal Degradation Activity and Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Romano,Paola Cordella,Cecilia Bucci

Communications biology 7:1088 PubMed39237682

2024

Lysosomal TMEM106B interacts with galactosylceramidase to regulate myelin lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Hideyuki Takahashi,Azucena Perez-Canamas,Chris W Lee,Hongping Ye,Xianlin Han,Stephen M Strittmatter

The Journal of biological chemistry 299:104916 PubMed37315786

2023

Disruption of Golgi markers by two RILP-directed shRNAs in neurons: A new role for RILP or a neuron-specific off-target phenotype?

Applications

Unspecified application

Species

Unspecified reactive species

Chan Choo Yap,Laura Digilio,Lloyd McMahon,Bettina Winckler

The Journal of biological chemistry 299:102883 PubMed36623732

2023

Loss of small GTPase Rab7 activation in prion infection negatively affects a feedback loop regulating neuronal cholesterol metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Pearl Cherry,Li Lu,Su Yeon Shim,Vincent Ebacher,Waqas Tahir,Hermann M Schatzl,Samia Hannaoui,Sabine Gilch

PLoS genetics 18:e1010232 PubMed35727824

2022

Dync1li1 is required for the survival of mammalian cochlear hair cells by regulating the transportation of autophagosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Shasha Zhang,Han Zhou,Xiangyu Ma,Leilei Wu,Mengyao Tian,Siyu Li,Xiaoyun Qian,Xia Gao,Renjie Chai

The Journal of cell biology 220: PubMed34014261

2021

Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney E Cason,Peter J Carman,Claire Van Duyne,Juliet Goldsmith,Roberto Dominguez,Erika L F Holzbaur

International journal of molecular sciences 22: PubMed33807278

2021

Rare Variants in Autophagy and Non-Autophagy Genes in Late-Onset Pompe Disease: Suggestions of Their Disease-Modifying Role in Two Italian Families.

Applications

Unspecified application

Species

Unspecified reactive species

Filomena Napolitano,Giorgia Bruno,Chiara Terracciano,Giuseppina Franzese,Nicole Piera Palomba,Federica Scotto di Carlo,Elisabetta Signoriello,Paolo De Blasiis,Stefano Navarro,Alessandro Gialluisi,Mariarosa Anna Beatrice Melone,Simone Sampaolo,Teresa Esposito

Journal of inherited metabolic disease 43:1082-1101 PubMed32279353

2020

Cln1-mutations suppress Rab7-RILP interaction and impair autophagy contributing to neuropathology in a mouse model of infantile neuronal ceroid lipofuscinosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chinmoy Sarkar,Tamal Sadhukhan,Maria B Bagh,Abhilash P Appu,Goutam Chandra,Avisek Mondal,Arjun Saha,Anil B Mukherjee

Frontiers in aging neuroscience 12:47 PubMed32210783

2020

Dihydroartemisinin Ameliorates Learning and Memory in Alzheimer's Disease Through Promoting Autophagosome-Lysosome Fusion and Autolysosomal Degradation for Aβ Clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyang Zhao,Zhimin Long,Ya Ding,Tingting Jiang,Jiajun Liu,Yimin Li,Yuanjie Liu,Xuehua Peng,Kejian Wang,Min Feng,Guiqiong He
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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