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AB302493

Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free)

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Rabbit Recombinant Monoclonal RILPL1 antibody. Carrier free. Suitable for WB, ICC/IF, IP and reacts with Human, Mouse, Rat samples.

View Alternative Names

RLP1, RILPL1, RILP-like protein 1, Rab-interacting lysosomal-like protein 1

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)

This data was developed using ab302492, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling RILPL1 with ab302492 at 1/250 (2.168 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing centrosome and cytoplasmic staining in SH-SY5Y cell line.Low expression : U-937 is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)

This data was developed using ab302492, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling RILPL1 with ab302492 at 1/250 (2.168 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing centrosome staining in Neuro-2a cell line. is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)
  • WB

Supplier Data

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)

All lanes:

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-ab302492'>ab302492</a>) at 1/1000 dilution

All lanes:

Wild-type A549 (human lu carcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Lanes 1 - 3:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

false

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)
  • WB

Supplier Data

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)

All lanes:

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-ab302492'>ab302492</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)
  • WB

Supplier Data

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)

All lanes:

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-ab302492'>ab302492</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human hypothalamus tissue lysate at 20 µg

Lane 3:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)
  • WB

Supplier Data

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)

All lanes:

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-ab302492'>ab302492</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 (human lu carcinoma epithelial cell), whole cell lysate 20 at 20 µg

Lane 2:

RILPL1 knockout A549 whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 4:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)
  • WB

Supplier Data

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (BSA and Azide free) (AB302493)

All lanes:

Western blot - Anti-RILPL1 antibody [MJF-R41-21] (<a href='/en-us/products/primary-antibodies/rilpl1-antibody-mjf-r41-21-ab302492'>ab302492</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MJF-R41-21

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Western blot - Anti-RAB10 (phospho T73) antibody [MJF-R21] (AB230261)

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  • 0
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Immunocytochemistry/ Immunofluorescence - Anti-gamma Tubulin antibody [EPR16793] - Centrosome Marker (AB179503)

  • 4
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Western blot - Anti-RAB29 (phospho T71) antibody [MJF-R24-17-1] (AB241062)

  • 0
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Reactivity data

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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RILPL1 also known as Rab interacting lysosomal protein-like 1 is a protein involved in cellular processes related to lysosomal trafficking. It has a molecular weight of approximately 45 kDa. RILPL1 expresses itself in a variety of tissues notably in the brain. The protein plays a significant role in intracellular transport mechanisms specifically in modulating the interaction between lysosomal structures and other cellular components.
Biological function summary

RILPL1 influences neuronal functions and is implicated in the regulation of ciliogenesis and other cellular morphogenesis processes. It often associates with components of the cytoskeletal network acting within protein complexes that contribute to cell shape and stability. This involves aiding in processes like axon transport which is critical for maintaining neuronal connectivity and function across different regions of the brain.

Pathways

RILPL1 is a part of cellular pathways involving lysosome positioning and transport. It interacts with motor proteins such as dynein and kinesin essential for microtubule-based movement. RILPL1's role in these pathways highlights its contribution to maintaining lysosomal homeostasis and transportation impacting processes like autophagy and endocytosis.

RILPL1 has been linked to neurodegenerative diseases like Huntington’s disease where disruption of lysosomal trafficking can lead to cellular pathology. The protein's interaction with the Huntingtin protein suggests its involvement in pathways that when dysregulated contribute to disease progression. Additionally irregularities in RILPL1 function may relate to ciliopathies disorders affecting cilia formation and function due to its role in driving key processes in neuronal cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the regulation of cell shape and polarity (By similarity). Plays a role in cellular protein transport, including protein transport away from primary cilia (By similarity). Neuroprotective protein, which acts by sequestring GAPDH in the cytosol and prevent the apoptotic function of GAPDH in the nucleus (By similarity). Competes with SIAH1 for binding GAPDH (By similarity). Does not regulate lysosomal morphology and distribution (PubMed : 14668488). Binds to RAB10 following LRRK2-mediated RAB10 phosphorylation which leads to inhibition of ciliogenesis (PubMed : 30398148).
See full target information RILPL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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