AB317450

Anti-RIM1 antibody [EPR29131-30]

BOND RX™ Validated
20ul selling size
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal RIMS1 antibody. Suitable for IHC-P, WB, IHC-Fr and reacts with Mouse, Rat, Human samples.

View Alternative Names

Kiaa0340, Rab3ip1, Rim1, Regulating synaptic membrane exocytosis protein 1, Rab-3-interacting molecule 1, Rab-3-interacting protein 1, RIM 1

17 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebrum.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cerebellum.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human liver.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Human hippocampus tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human hippocampus.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh frozen) tissue labeling RIM1 with ab317450 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Negative control : confocal image showing no staining on rat liver. The nuclear counterstain was DAPI (Blue). The section was incubated with ab317450 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebellum (PMID : 17124501).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebellum (PMID : 10748113).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebrum (PMID : 17124501).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse hippocampus (PMID : 10748113).
The section was incubated with ab317450for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling RIM1 with ab317450 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Panel A : merged staining of anti-RIMS1 (ab317450, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, magenta) on rat hippocampus.
Panel B : anti-RIMS1 stained on rat hippocampus.
Panel C : anti-NeuN stained in neurons of rat hippocampus.
Panel D : anti-GFAP stained in astrocytes of rat hippocampus.
The section was incubated in two rounds of staining : in the order of ab317450 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling RIM1 with ab317450 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Panel A : merged staining of anti-RIMS1 (ab317450, green), anti-NeuN (ab190565, grey) and anti-GFAP (ab201732, magenta) on mouse hippocampus.
Panel B : anti-RIMS1 stained on mouse hippocampus.
Panel C : anti-NeuN stained in neurons of mouse hippocampus.
Panel D : anti-GFAP stained in astrocytes of mouse hippocampus.
The section was incubated in two rounds of staining : in the order of ab317450 and ab190565, ab201732 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum (PMID : 10748113).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse liver (PMID : 10748113).
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling RIM1 with ab317450 at 1/100 (5.03 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on rat liver.
The section was incubated with ab317450 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with citrate buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh frozen) tissue labeling RIM1 with ab317450 at 1/50 (10.06 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Negative control : confocal image showing no staining on mouse liver (PMID : 10748113). The nuclear counterstain was DAPI (Blue). The section was incubated with ab317450 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 ug/mL) dilution.

Supplier Data

Western blot - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Negative control : liver (PMID : 10748113).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10748113, PMID : 19074017, PMID : 28701482).

The bands beneath the target band (130 kDa) are likely to be degraded target fragments.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-RIM1 antibody [EPR29131-30] (ab317450) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human hippocampus tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 130-200 kDa,124 kDa

true

Exposure time: 180s

Supplier Data

Western blot - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Negative control : liver, kidney (PMID : 10748113).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10748113, PMID : 19074017, PMID : 28701482).

In lanes 1-9, the lysates were stored at -80°C prior to Western Blotting. The bands beneath the target band (130 kDa) are likey to be degradation products. In lanes 10-13, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lanes 1-9 : 26 seconds, lane 10 : 37 seconds, lane 11 : 180 seconds, lanes 12-13 : 48 seconds

All lanes:

Western blot - Anti-RIM1 antibody [EPR29131-30] (ab317450) at 1/1000 dilution

Lanes 1 and 10:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse striatum tissue lysate at 20 µg

Lanes 4 and 11:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse kidney tissue lysate at 20 µg

Lanes 7 and 12:

Rat brain tissue lysate at 20 µg

Lane 8:

Rat cerebellum tissue lysate at 20 µg

Lanes 9 and 13:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-200 kDa,124 kDa

false

Carrier free

Anti-RIM1 antibody [EPR29131-30] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29131-30

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIM1 also known as Rab3-interacting molecule 1 is a protein with a mass of approximately 200 kDa. It serves as an important component in the exocytosis process by regulating synaptic vesicle release in the presynaptic terminal. RIM1 interacts with various proteins such as Munc13-1 and is integral to synaptic vesicle docking and priming. This protein predominantly expresses in the brain especially in areas with high synaptic transmission needs like the hippocampus and cortex.

Biological function summary

This protein acts as an organizer of active zones in the presynaptic neuron ensuring efficient neurotransmitter release. RIM1 is part of a larger complex known as the presynaptic active zone complex which includes other critical proteins like RIM-BP and ELKS. These interactions stabilize synaptic function and facilitate rapid neurotransmission. Additionally RIM1 has a role in modulating calcium channel activity which is essential for triggering synaptic vesicle fusion.

Pathways

Various neurological signaling processes rely on the function of RIM1. This protein plays important roles in the pathways of synaptic vesicle cycling and neurotransmitter release directly influencing synaptic strength and plasticity. RIM1 closely interacts with the Rab3 GDP-GTP cycle and Munc13 both of which participate in neurotransmitter release processes. Proper function of these pathways ensures precise neuronal communication and adaptation.

Any malfunction or mutation in RIM1 can significantly impact neurological health. The protein is linked to several neuropsychiatric disorders such as autism spectrum disorder and epilepsy. Researchers observed that disrupted RIM1 function correlates with aberrant synaptic transmission that can contribute to these conditions. In the context of epilepsy changes in RIM1 interaction with synaptic partners like RIM-BP and Munc13 can lead to irregular neuronal firing and seizures.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Rab effector involved in exocytosis (PubMed : 11797009). May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity (PubMed : 11797009). Plays a role in dendrite formation by melanocytes (By similarity).

See full target information Rims1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RIM1 antibody [EPR29131-30]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Frozen sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Frozen sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Frozen sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Immunohistochemistry (Frozen sections) - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Western blot - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Western blot - Anti-RIM1 antibody [EPR29131-30] (AB317450)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise