Rabbit Recombinant Monoclonal RIMS1 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Transfected cell line - Mouse, Mouse, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430101.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328)")
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430095.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328)")
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430100.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328)")
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430099.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328)")
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430098.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (AB317328)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | IHC-P | WB | IP | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Tested | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Mouse | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse, Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell line - Mouse, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Transfected cell line - Mouse, Human | Dilution info - | Notes - |
Target data
Function
Rab effector involved in exocytosis (PubMed:11797009). May act as scaffold protein that regulates neurotransmitter release at the active zone. Essential for maintaining normal probability of neurotransmitter release and for regulating release during short-term synaptic plasticity (PubMed:11797009). Plays a role in dendrite formation by melanocytes (By similarity).
Alternative names
Kiaa0340, Rab3ip1, Rim1, Regulating synaptic membrane exocytosis protein 1, Rab-3-interacting molecule 1, Rab-3-interacting protein 1, RIM 1
Recommended products
Rabbit Recombinant Monoclonal RIMS1 antibody. Carrier free. Suitable for ICC/IF, Flow Cyt and reacts with Transfected cell line - Mouse, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29131-34
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317328 is the carrier-free version of Anti-RIM1 antibody [EPR29131-34] ab317327.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RIM1 also known as Rab3-interacting molecule 1 is a protein with a mass of approximately 200 kDa. It serves as an important component in the exocytosis process by regulating synaptic vesicle release in the presynaptic terminal. RIM1 interacts with various proteins such as Munc13-1 and is integral to synaptic vesicle docking and priming. This protein predominantly expresses in the brain especially in areas with high synaptic transmission needs like the hippocampus and cortex.
- Biological function summary
This protein acts as an organizer of active zones in the presynaptic neuron ensuring efficient neurotransmitter release. RIM1 is part of a larger complex known as the presynaptic active zone complex which includes other critical proteins like RIM-BP and ELKS. These interactions stabilize synaptic function and facilitate rapid neurotransmission. Additionally RIM1 has a role in modulating calcium channel activity which is essential for triggering synaptic vesicle fusion.
- Pathways
Various neurological signaling processes rely on the function of RIM1. This protein plays important roles in the pathways of synaptic vesicle cycling and neurotransmitter release directly influencing synaptic strength and plasticity. RIM1 closely interacts with the Rab3 GDP-GTP cycle and Munc13 both of which participate in neurotransmitter release processes. Proper function of these pathways ensures precise neuronal communication and adaptation.
- Associated diseases and disorders
Any malfunction or mutation in RIM1 can significantly impact neurological health. The protein is linked to several neuropsychiatric disorders such as autism spectrum disorder and epilepsy. Researchers observed that disrupted RIM1 function correlates with aberrant synaptic transmission that can contribute to these conditions. In the context of epilepsy changes in RIM1 interaction with synaptic partners like RIM-BP and Munc13 can lead to irregular neuronal firing and seizures.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430101.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
This data was developed using Anti-RIM1 antibody [EPR29131-34] ab317327, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling RIM1 with Anti-RIM1 antibody [EPR29131-34] ab317327 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in 293T cells transfected with a mouse RIMS1 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430095.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
This data was developed using Anti-RIM1 antibody [EPR29131-34] ab317327, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling RIM1 with Anti-RIM1 antibody [EPR29131-34] ab317327 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in mouse primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430100.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
This data was developed using Anti-RIM1 antibody [EPR29131-34] ab317327, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat bone marrow cells labelling RIM1 with Anti-RIM1 antibody [EPR29131-34] ab317327 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Negative control: Confocal image showing no staining in rat bone marrow. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430099.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
This data was developed using Anti-RIM1 antibody [EPR29131-34] ab317327, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Rat splenocyte cells labelling RIM1 with Anti-RIM1 antibody [EPR29131-34] ab317327 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Negative control: Confocal image showing no staining in rat splenocytes. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430098.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
This data was developed using Anti-RIM1 antibody [EPR29131-34] ab317327, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling RIM1 with Anti-RIM1 antibody [EPR29131-34] ab317327 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing positive staining in rat primary neuron (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430097.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
This data was developed using Anti-RIM1 antibody [EPR29131-34] ab317327, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse bone marrow cells labelling RIM1 with Anti-RIM1 antibody [EPR29131-34] ab317327 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Negative control: Confocal image showing no staining in mouse bone marrow. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430096.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
This data was developed using Anti-RIM1 antibody [EPR29131-34] ab317327, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocyte cells labelling RIM1 with Anti-RIM1 antibody [EPR29131-34] ab317327 at 1/100 (5.17 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Negative control: Confocal image showing no staining in mouse splenocytes. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--flow-cytometry-img430189.jpg "Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
This data was developed using Anti-RIM1 antibody [EPR29131-34] ab317327, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse spleen cell cells labelling RIM1 with Anti-RIM1 antibody [EPR29131-34] ab317327 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: mouse spleen cell
![Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--flow-cytometry-img430188.jpg "Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)
This data was developed using Anti-RIM1 antibody [EPR29131-34] ab317327, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse primary neuron cells labelling RIM1 with Anti-RIM1 antibody [EPR29131-34] ab317327 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430097.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
![Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--immunocytochemistry-immunofluorescence-img430096.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
![Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--flow-cytometry-img430189.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
![Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328), expandable thumbnail](https://content.abcam.com/products/images/rim1-antibody-epr29131-34-bsa-and-azide-free-ab317328--flow-cytometry-img430188.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-RIM1 antibody [EPR29131-34] - BSA and Azide free (ab317328)")
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