JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251138

Anti-RIMS3 antibody [EPR10117(2)] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal RIMS3 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

KIAA0237, RIMS3, Regulating synaptic membrane exocytosis protein 3, Nim3, RIM3 gamma, Rab-3-interacting molecule 3, RIM 3

2 Images
Western blot - Anti-RIMS3 antibody [EPR10117(2)] - BSA and Azide free (AB251138)
  • WB

Supplier Data

Western blot - Anti-RIMS3 antibody [EPR10117(2)] - BSA and Azide free (AB251138)

This data was developed using ab192602, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RIMS3 antibody [EPR10117(2)] (<a href='/en-us/products/primary-antibodies/rims3-antibody-epr101172-ab192602'>ab192602</a>) at 1/10000 dilution

Lane 1:

Human cerebellum lysate at 20 µg

Lane 2:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 40 kDa

true

Western blot - Anti-RIMS3 antibody [EPR10117(2)] - BSA and Azide free (AB251138)
  • WB

Supplier Data

Western blot - Anti-RIMS3 antibody [EPR10117(2)] - BSA and Azide free (AB251138)

This data was developed using ab192602, the same antibody clone in a different buffer formulation.

It is not sure that if the band at about 30 kDa is the extra band.

All lanes:

Western blot - Anti-RIMS3 antibody [EPR10117(2)] (<a href='/en-us/products/primary-antibodies/rims3-antibody-epr101172-ab192602'>ab192602</a>) at 1/1000 dilution

Lane 1:

mouse brain lysate at 10 µg

Lane 2:

rat brain lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa

Observed band size: 40 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10117(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB251434

Anti-KA1 antibody [EPR18322] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

  • 0
  • 0
Primary Antibodies

AB221547

Anti-ZO1 tight junction protein antibody [EPR19945-296]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZO1 tight junction protein antibody [EPR19945-296] (AB221547)

  • 5
  • 6
Primary Antibodies

AB191385

Anti-SIRT6 antibody [EPR18463]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-SIRT6 antibody [EPR18463] (AB191385)

  • 5
  • 2
Primary Antibodies

AB32352

Anti-HIV1 p24 antibody [P131]

RabMAb|Recombinant

Western blot - Anti-HIV1 p24 antibody [P131] (AB32352)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab251138 is the carrier-free version of ab192602.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIMS3 also known as Regulating Synaptic Membrane Exocytosis 3 is a protein around 170 kDa in size. It plays a role in the modulation of synaptic vesicle exocytosis. RIMS3 is expressed mainly in neuronal tissues and shows localization at presynaptic active zones. This protein is part of the Rab3-interacting molecule family which contributes to the intricate machinery involved in neurotransmitter release at synapses by interacting with other proteins important for synaptic transmission.
Biological function summary

The function of RIMS3 revolves around its role in synaptic processes. RIMS3 contributes to the regulation of synaptic vesicle priming and neurotransmitter release. It functions as part of a protein complex that includes other synaptic proteins like Munc13 and RIM-binding protein. These interactions stabilize synaptic transmission and maintain precise communication between neurons. The absence or alteration of RIMS3 can disrupt these processes and result in altered synaptic strength and plasticity.

Pathways

RIMS3 participates in synaptic vesicle cycle and neurotransmitter release pathways. It interacts with members of the Rab family such as Rab3 playing a part in the vesicle docking and priming steps critical for synaptic activity. Through these pathways RIMS3 aligns functionally with related proteins like Synaptotagmin and SNARE complex components amplifying the efficiency of synaptic vesicle release which underpins neuronal communication and network activity.

Alterations in RIMS3 expression associate with neurological conditions such as autism spectrum disorder (ASD) and schizophrenia. Mutations or disruptions in RIMS3 or its related pathways can affect synaptic efficiency contributing to these disorders. Other synaptic proteins like Neurexin and Neuroligin also connect to these synaptic deficits reinforcing the link between proper RIMS3 function and neural health. Understanding the precise involvement of RIMS3 in these diseases may guide therapeutic approaches aimed at restoring normal synaptic function.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Regulates synaptic membrane exocytosis.
See full target information RIMS3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34769092

2021

11,12 Epoxyeicosatrienoic Acid Rescues Deteriorated Wound Healing in Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Sommer,Heike Jakob,Caroline Reiche,Dirk Henrich,Jasmina Sterz,Johannes Frank,Ingo Marzi,Anna Lena Sander
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com