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AB64838

Anti-RIN3 antibody

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(2 Publications)

Rabbit Polyclonal RIN3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Ras and Rab interactor 3, Ras interaction/interference protein 3, RIN3

1 Images
Western blot - Anti-RIN3 antibody (AB64838)
  • WB

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Western blot - Anti-RIN3 antibody (AB64838)

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

All lanes:

Western blot - Anti-RIN3 antibody (ab64838) at 1/500 dilution

Lane 1:

HUVEC cell extract

Lane 2:

K562 cell extract

Lane 3:

HUVEC cell extract with Immunising peptide

Predicted band size: 108 kDa

Observed band size: 108 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIN3 also known as Ras and Rab interactor 3 is a member of the RIN family of proteins. This protein has a mass of approximately 100 kDa. RIN3 functions as a GDP-GTP exchange factor which is important for the regulation of endocytosis and actin cytoskeleton organization. It primarily helps in activating small GTPase proteins particularly RAB5 and RAB31. RIN3 expression is found in various tissues with higher levels noted in bone and immune-related tissues.
Biological function summary

This protein influences several important cellular processes including intracellular trafficking and signal transduction. It does not form large complexes itself but it interacts with other proteins like BIN2 which facilitates endocytic vesicle formation and cellular signaling. By these interactions RIN3 contributes to actin cytoskeleton rearrangement and membrane ruffling impacting the cellular uptake and movement.

Pathways

Significant involvement occurs in the endocytic pathway and actin cytoskeleton regulatory pathway. Within the endocytic pathway RIN3 interacts with RAB5 and other related GTPases such as RAB31 orchestrating early endosome dynamics. In the actin cytoskeleton pathway interactions with BIN2 play a role in actin filament modulation which influences cell shape and motility.

RIN3 has been linked to Paget's Disease of Bone and some forms of immunodeficiency. This protein's dysfunction in these conditions relates to its role in bone resorption and immune cell function. Additionally it interacts with SQSTM1 which is associated with bone metabolism and STK11 which relates to immune function disorders. Understanding these interactions and pathways provides insight into RIN3's involvement in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ras effector protein that functions as a guanine nucleotide exchange (GEF) for RAB5B and RAB31, by exchanging bound GDP for free GTP. Required for normal RAB31 function.
See full target information RIN3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Translational neurodegeneration 9:26 PubMed32552912

2020

Upregulation of RIN3 induces endosomal dysfunction in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ruinan Shen,Xiaobei Zhao,Lu He,Yongbo Ding,Wei Xu,Suzhen Lin,Savannah Fang,Wanlin Yang,Kijung Sung,Brian Spencer,Robert A Rissman,Ming Lei,Jianqing Ding,Chengbiao Wu

Molecular and cellular biology 34:4130-42 PubMed25182532

2014

MicroRNA 34a inhibits beige and brown fat formation in obesity in part by suppressing adipocyte fibroblast growth factor 21 signaling and SIRT1 function.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Fu,Sunmi Seok,Sunge Choi,Zhang Huang,Kelly Suino-Powell,H Eric Xu,Byron Kemper,Jongsook Kim Kemper
View all publications
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