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AB180170

Anti-RING1 antibody [EPR13047]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal RING1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

RING1A, RNF1, RING1, E3 ubiquitin-protein ligase RING1, Polycomb complex protein RING1, RING finger protein 1, RING-type E3 ubiquitin transferase RING1, Really interesting new gene 1 protein

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [EPR13047] (AB180170)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RING1 antibody [EPR13047] (AB180170)

Immunofluorescence analysis of HeLa cells labeling RING1 using ab180170 at 1/50 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (AB180170)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (AB180170)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling RING1 using ab180170 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (AB180170)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING1 antibody [EPR13047] (AB180170)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling RING1 using ab180170 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-RING1 antibody [EPR13047] (AB180170)
  • IP

Lab

Immunoprecipitation - Anti-RING1 antibody [EPR13047] (AB180170)

RING1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab180170 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab180170 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/10000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab180170 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab180170 in mouse cerebellum tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds

All lanes:

Immunoprecipitation - Anti-RING1 antibody [EPR13047] (ab180170)

Predicted band size: 42 kDa

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Western blot - Anti-RING1 antibody [EPR13047] (AB180170)
  • WB

Lab

Western blot - Anti-RING1 antibody [EPR13047] (AB180170)

Blocking and diluting buffer and concentration 5% NFDM/TBST.

Exposure Time :
Lane 1-3 : 3 minutes;
Lane 4 : 1 minute

All lanes:

Western blot - Anti-RING1 antibody [EPR13047] (ab180170) at 1/5000 dilution

Lane 1:

Ramos (human Burkitt's lymphoma) whole cell lysate at 10 µg

Lane 2:

MOLT-4 (human acute lymphoblastic leukemia) whole cell lysate at 10 µg

Lane 3:

LNCaP (Human prostate carcinoma) whole cell lysate at 10 µg

Lane 4:

HeLa (human cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

Predicted band size: 42 kDa

Observed band size: 50 kDa

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Western blot - Anti-RING1 antibody [EPR13047] (AB180170)
  • WB

Lab

Western blot - Anti-RING1 antibody [EPR13047] (AB180170)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : RING1 knockout HAP1 cell lysate (20 μg)
Lane 3 : MOLT4 cell lysate (20 μg)
Lane 4 : LnCaP cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab180170 observed at 52 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab180170 was shown to specifically react with RING1 when RING1 knockout samples were used. Wild-type and RING1 knockout samples were subjected to SDS-PAGE. ab180170 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-RING1 antibody [EPR13047] (ab180170)

Predicted band size: 42 kDa

false

  • Carrier free

    Anti-RING1 antibody [EPR13047] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13047

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RING1 also known as Ring Finger Protein 1 or RNF1 is an E3 ubiquitin-protein ligase with a molecular mass of approximately 42 kDa. It is part of the polycomb group proteins known for regulating gene expression. RING1 is expressed ubiquitously in various tissues where it functions as an important regulator of protein degradation by tagging substrates with ubiquitin for proteasomal degradation. This activity plays a critical role in maintaining cellular homeostasis by controlling the abundance of regulatory proteins.
Biological function summary

Along with modulating protein turnover RING1 participates as a fundamental component of the Polycomb Repressive Complex 1 (PRC1). This complex is imperative in chromatin remodeling and transcriptional repression influencing epigenetic gene silencing. RING1 works in tandem with other proteins such as BMI1 facilitating histone ubiquitination and altering chromatin structure which impacts gene expression across different cell types and developmental stages.

Pathways

The ubiquitin-proteasome system and the epigenetic regulation pathway involve RING1. Within the ubiquitin-proteasome system RING1 interacts with other E3 ligases and ubiquitin-conjugating enzymes governing the fate of its protein substrates. In the epigenetic regulation pathway RING1's role in PRC1 places it alongside proteins like EZH2 contributing to the repression of genes involved in differentiation and development thereby ensuring proper cell identity maintenance.

Mutations or misregulation of RING1 have links to several conditions including cancers and neurodegenerative disorders. In cancer the disruption of normal RING1 functions can lead to unregulated cell proliferation due to improper gene silencing. For neurodegenerative disorders associations with proteins such as ubiquitin and its regulatory roles may contribute to disease pathogenesis by enabling the accumulation of misfolded proteins further highlighting its significance in cellular health and disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
See full target information RING1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 24:102435 PubMed34113809

2021

The USP7-TRIM27 axis mediates non-canonical PRC1.1 function and is a druggable target in leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Henny Maat,Tjerk Jan Atsma,Shanna M Hogeling,Aida Rodríguez López,Jennifer Jaques,Mirjam Olthuis,Marcel P de Vries,Chantal Gravesteijn,Annet Z Brouwers-Vos,Nisha van der Meer,Suzan Datema,Jonas Salzbrunn,Gerwin Huls,Roy Baas,Joost H A Martens,Vincent van den Boom,Jan Jacob Schuringa

Medical science monitor : international medical jo 22:2357-62 PubMed27385084

2016

Thymic Stromal Lymphopoietin Promotes Fibrosis and Activates Mitogen-Activated Protein Kinases in MRC-5 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Su Tang,Xiaodong Tang
View all publications
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Product promise

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