Rabbit Polyclonal RING2 / RING1B / RNF2 antibody. Suitable for IHC-P, IP, WB, ICC/IF, ChIP-seq and reacts with Human, Mouse samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human RNF2 aa 150-200.
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Publications
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- eLife 11:2022PRC1 sustains the integrity of neural fate in the absence of PRC2 function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyana Sawai,Sarah Pfennig,Milica Bulajić,Alexander Miller,Alireza Khodadadi-Jamayran,Esteban O Mazzoni,Jeremy S DasenPubMed 34994686
- Nature communications 12:54022021Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMathieu F Bakhoum,Jasmine H Francis,Albert Agustinus,Ethan M Earlie,Melody Di Bona,David H Abramson,Mercedes Duran,Ignas Masilionis,Elsa Molina,Alexander N Shoushtari,Michael H Goldbaum,Paul S Mischel,Samuel F Bakhoum,Ashley M LaughneyPubMed 34518527
- Science advances 7:2021The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBastian Stielow,Yuqiao Zhou,Yinghua Cao,Clara Simon,Hans-Martin Pogoda,Junyi Jiang,Yanpeng Ren,Sabrina Keita Phanor,Iris Rohner,Andrea Nist,Thorsten Stiewe,Matthias Hammerschmidt,Yang Shi,Martha L Bulyk,Zhanxin Wang,Robert LiefkePubMed 33980486
- Scientific reports 11:98132021In vivo cell proliferation analysis and cell-tracing reveal the global cellular dynamics of periodontal ligament cells under mechanical-loading.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasaru Mizukoshi,Masaru Kaku,Lay Thant,Kohei Kitami,Moe Arai,Isao Saito,Katsumi UoshimaPubMed 33963224
- Methods in molecular biology (Clifton, N.J.) 2520:117-1332021ChIP-qPCR for Polycomb Group Proteins During Neuronal Differentiation of Human Pluripotent Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDivya Desai,Prasad PethePubMed 33945141
- Aging 12:26199-262202020Ring finger protein 2 promotes colorectal cancer progression by suppressing early growth response 1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeilong Wei et. al.PubMed 33346749
- Oncology letters 20:1111-11182020Clinical significance of ring finger protein 2 high expression in skin squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBai Ling et. al.PubMed 32724350
- Cell chemical biology 26:1365-1379.e222019Discovery and Characterization of a Cellular Potent Positive Allosteric Modulator of the Polycomb Repressive Complex 1 Chromodomain, CBX7.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelsey N Lamb et. al.PubMed 31422906
- Oncology letters 18:3734-37422019Association between RNF2+P-AKT expression in pretreatment biopsy specimens, and poor survival following radiotherapy in patients with esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiaofang Li,Shuguang Li,Xingxiao Yang,Xueyuan Zhang,Chunyang Song,Shuchai ZhuPubMed 31516586
- The Journal of biological chemistry 293:19250-192622018Transcription factor Sp2 potentiates binding of the TALE homeoproteins Pbx1:Prep1 and the histone-fold domain protein Nf-y to composite genomic sites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Völkel et. al.PubMed 30337366
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RNF2 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q99496
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Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | ChIP-seq | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Expected |
| Mouse | Predicted | Predicted | Predicted | Expected | Expected |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted | Predicted |
| Platypus | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted | Predicted | Predicted |
| Turkey | Predicted | Predicted | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Use with methanol or paraformaldehyde fixed cells. |
Species Human | Dilution info - | Notes Use with methanol or paraformaldehyde fixed cells. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus | Dilution info - | Notes - |
ChIP-seq
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes 10 μg |
Species Human | Dilution info - | Notes 10 μg |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus | Dilution info - | Notes - |
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Target data
Function
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation (PubMed:15386022, PubMed:16359901, PubMed:21772249, PubMed:25355358, PubMed:25519132, PubMed:26151332, PubMed:33864376). H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation (By similarity). Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:16359901, PubMed:26151332). PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed:26151332). E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed:21772249). Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (By similarity). Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation (By similarity).
Alternative names
BAP1, DING, HIPI3, RING1B, RNF2, E3 ubiquitin-protein ligase RING2, Huntingtin-interacting protein 2-interacting protein 3, Protein DinG, RING finger protein 1B, RING finger protein 2, RING finger protein BAP-1, RING-type E3 ubiquitin transferase RING2, HIP2-interacting protein 3, RING1b
Recommended products
Rabbit Polyclonal RING2 / RING1B / RNF2 antibody. Suitable for IHC-P, IP, WB, ICC/IF, ChIP-seq and reacts with Human, Mouse samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human RNF2 aa 150-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RNF2 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q99496
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RING2 also known as RING1B or RNF2 is a protein that functions as a member of the Polycomb Repressive Complex 1 (PRC1). This protein contains a RING-finger domain facilitating ubiquitin ligase activity. It has a molecular mass of approximately 38 kDa. RING2/RNF2/RING1B is expressed in various tissues including the brain heart and lungs indicating its fundamental role across different biological systems.
- Biological function summary
RING2/RNF2/RING1B regulates gene expression by modifying chromatin structure especially through monoubiquitination of histone H2A at lysine 119. This action occurs within the Polycomb Repressive Complex 1 a multiprotein structure important for maintaining the transcriptional repression of target genes. It influences cell cycle progression and development by silencing genes involved in proliferation and differentiation.
- Pathways
RING2/RNF2/RING1B's role connects to the Polycomb group (PcG) pathway and the Wnt signaling pathway. Within these pathways it interacts with proteins like BMI1 and EZH2 which are other components of polycomb complexes. These interactions help modulate chromatin dynamics and control the expression of several genes involved in cell identity and development.
- Associated diseases and disorders
Dysfunction or aberrant expression of RING2/RNF2/RING1B has links to cancer and neurological disorders. Its relationship with proteins like CBX8 ties it to pathways of oncogenesis where it can influence tumorigenesis through misregulation of gene silencing. Furthermore changes in RING2/RNF2/RING1B expression or function may contribute to developmental disorders associated with improper neuronal development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-RING2 / RING1B / RNF2 antibody (ab101273)
All lanes: Western blot - Anti-RING2 / RING1B / RNF2 antibody (ab101273) at 0.1 µg/mL
Lane 1: K562 whole cell lysate at 50 µg
Lane 2: K562 whole cell lysate at 15 µg
Lane 3: K562 whole cell lysate at 5 µg
Lane 4: 293T whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30s
Immunoprecipitation - Anti-RING2 / RING1B / RNF2 antibody (ab101273)
Detection of RING2 / RING1B / RNF2 in Immunoprecipitates of K562 whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101273 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-RING2 / RING1B / RNF2 antibody (ab101273)
Predicted band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (ab101273)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling RING2 / RING1B / RNF2 with ab101273 at 1/1000 (1µg/ml).
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