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AB187509

Anti-RING2 / RING1B / RNF2 antibody

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(3 Publications)

Rabbit Polyclonal RING2 / RING1B / RNF2 antibody. Suitable for IP, ChIP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RNF2.

View Alternative Names

BAP1, DING, HIPI3, RING1B, RNF2, E3 ubiquitin-protein ligase RING2, Huntingtin-interacting protein 2-interacting protein 3, Protein DinG, RING finger protein 1B, RING finger protein 2, RING finger protein BAP-1, RING-type E3 ubiquitin transferase RING2, HIP2-interacting protein 3, RING1b

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (AB187509)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (AB187509)

Paraffin-embedded human gastric cancer tissue stained for RING2 / RING1B / RNF2 using ab187509 at 1/100 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-RING2 / RING1B / RNF2 antibody (AB187509)
  • IP

Supplier Data

Immunoprecipitation - Anti-RING2 / RING1B / RNF2 antibody (AB187509)

Immunoprecipitation analysis of 200μg extracts of 293T cells using 1μg of ab187509. Western blot was performed from the immunoprecipitate using ab187509 at a dilution of 1 : 1000.

All lanes:

Immunoprecipitation - Anti-RING2 / RING1B / RNF2 antibody (ab187509)

Predicted band size: 37 kDa

false

ChIP - Anti-RING2 / RING1B / RNF2 antibody (AB187509)
  • ChIP

Supplier Data

ChIP - Anti-RING2 / RING1B / RNF2 antibody (AB187509)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using ab187509 and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (AB187509)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (AB187509)

Paraffin-embedded mouse brain tissue stained for RING2 / RING1B / RNF2 using ab187509 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (AB187509)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RING2 / RING1B / RNF2 antibody (AB187509)

Paraffin-embedded rat lung tissue stained for RING2 / RING1B / RNF2 using ab187509 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-RING2 / RING1B / RNF2 antibody (AB187509)
  • WB

Supplier Data

Western blot - Anti-RING2 / RING1B / RNF2 antibody (AB187509)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-RING2 / RING1B / RNF2 antibody (ab187509) at 1/1000 dilution

Lane 1:

293T cell lysate at 25 µg

Lane 2:

Jurkat cell lysate at 25 µg

Lane 3:

U-937 cell lysate at 25 µg

Lane 4:

SH-SY5Y cell lysate at 25 µg

Lane 5:

Mouse brain tissue lysate at 25 µg

Lane 6:

Mouse spleen tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 37 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, ChIP, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RNF2.

Q99496

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/200", "IP-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1/50 - 1/200", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RING2 also known as RING1B or RNF2 is a protein that functions as a member of the Polycomb Repressive Complex 1 (PRC1). This protein contains a RING-finger domain facilitating ubiquitin ligase activity. It has a molecular mass of approximately 38 kDa. RING2/RNF2/RING1B is expressed in various tissues including the brain heart and lungs indicating its fundamental role across different biological systems.
Biological function summary

RING2/RNF2/RING1B regulates gene expression by modifying chromatin structure especially through monoubiquitination of histone H2A at lysine 119. This action occurs within the Polycomb Repressive Complex 1 a multiprotein structure important for maintaining the transcriptional repression of target genes. It influences cell cycle progression and development by silencing genes involved in proliferation and differentiation.

Pathways

RING2/RNF2/RING1B's role connects to the Polycomb group (PcG) pathway and the Wnt signaling pathway. Within these pathways it interacts with proteins like BMI1 and EZH2 which are other components of polycomb complexes. These interactions help modulate chromatin dynamics and control the expression of several genes involved in cell identity and development.

Dysfunction or aberrant expression of RING2/RNF2/RING1B has links to cancer and neurological disorders. Its relationship with proteins like CBX8 ties it to pathways of oncogenesis where it can influence tumorigenesis through misregulation of gene silencing. Furthermore changes in RING2/RNF2/RING1B expression or function may contribute to developmental disorders associated with improper neuronal development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation (PubMed : 15386022, PubMed : 16359901, PubMed : 21772249, PubMed : 25355358, PubMed : 25519132, PubMed : 26151332, PubMed : 33864376). H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation (By similarity). Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed : 16359901, PubMed : 26151332). PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed : 26151332). E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed : 21772249). Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (By similarity). Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation (By similarity).
See full target information RNF2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & therapeutics 33:117-128 PubMed39632667

2024

Glutathione's Role in Liver Metabolism and Hangover Symptom Relief: Dysregulation of Protein S-Glutathionylation and Antioxidant Enzymes.

Applications

Unspecified application

Species

Unspecified reactive species

Hwa-Young Lee,Geum-Hwa Lee,Do-Sung Kim,Young Jae Lim,Boram Cho,Hojung Jung,Hyun-Shik Choi,Soonok Sa,Wookyung Chung,Hyewon Lee,Myoung Ja Chung,Junghyun Kim,Han-Jung Chae

Cancer & metabolism 12:30 PubMed39456039

2024

RNF2 promotes chondrosarcoma progression by regulating ubiquitination and degradation of CBX7.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Wu,Zheng Huang,Ping Luo,Zhong Xiang,Meng Zhang,Zhiwu Chen,Yalu Zhou,Jiameng Li

Life science alliance 4: PubMed33361335

2020

The chromatin landscape of primary synovial sarcoma organoids is linked to specific epigenetic mechanisms and dependencies.

Applications

Unspecified application

Species

Unspecified reactive species

Gaylor Boulay,Luisa Cironi,Sara P Garcia,Shruthi Rengarajan,Yu-Hang Xing,Lukuo Lee,Mary E Awad,Beverly Naigles,Sowmya Iyer,Liliane C Broye,Tugba Keskin,Alexandra Cauderay,Carlo Fusco,Igor Letovanec,Ivan Chebib,Petur Gunnalugur Nielsen,Stéphane Tercier,Stéphane Cherix,Tu Nguyen-Ngoc,Gregory Cote,Edwin Choy,Paolo Provero,Mario L Suvà,Miguel N Rivera,Ivan Stamenkovic,Nicolò Riggi
View all publications
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Product promise

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