AB3832

Anti-RING2 / RING1B / RNF2 antibody

Name: Anti-RING2 / RING1B / RNF2 antibody (ab3832) | Abcam
Brand: Abcam Limited
SKU: AB3832
Price: 490 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(25 Publications)

Goat Polyclonal RING2 / RING1B / RNF2 antibody. Suitable for WB and reacts with Chinese hamster, Mouse, Human samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Human RNF2 aa 150-250.

View Alternative Names

BAP1, DING, HIPI3, RING1B, RNF2, E3 ubiquitin-protein ligase RING2, Huntingtin-interacting protein 2-interacting protein 3, Protein DinG, RING finger protein 1B, RING finger protein 2, RING finger protein BAP-1, RING-type E3 ubiquitin transferase RING2, HIP2-interacting protein 3, RING1b

2 Images

Unknown

Western blot - Anti-RING2 / RING1B / RNF2 antibody (AB3832)

Western blot using ab3832 at 1/500. Lysates at 20μg/lane.

Lane 1 : 3T3 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : CHO-K1 cell lysate

Secondary ab : rabbit polyclonal to goat IgG ab6741 1/5000

Exposure time : 3 minutes

All lanes:

Western blot - Anti-RING2 / RING1B / RNF2 antibody (ab3832)

Predicted band size: 37 kDa

false

CiteAb

Western blot - Anti-RING2 / RING1B / RNF2 antibody (AB3832)

RING2 / RING1B / RNF2 western blot using anti-RING2 / RING1B / RNF2 antibody ab3832. Publication image and figure legend from Toth, Z., Papp, B., et al., 2016, PLoS Pathog, PubMed 27606464.

ab3832 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab3832 please see the product overview.

Interaction of LANA with PRC2.(A) Gel filtration chromatography of nuclear extracts derived from iSLK and iSLKBAC16 cells. Fractions from 28 to 72 were tested by immunoblot analysis for LANA and polycomb proteins. Molecular weight markers are indicated. (B) Co-immunoprecipitation of LANA-V5 (using V5 antibody) from 293T cells expressing LANA-V5 and the indicated 3xFLAG-tagged PRC2 (EZH2, SUZ12 and EED) or PRC1 (RING1B and BMI-1) factors. Immunoprecipitation and input blots were probed with V5 and FLAG antibodies. (C) Cell lysates derived from de novo KSHV infected iSLK cells (72 hpi) were used for immunoprecipitation with anti-EZH2 rabbit antibody (αEZH2) or normal rabbit IgG (αIgG), followed by immunoblot with αLANA and αEZH2.

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Chinese hamster

Applications

applications

Immunogen

Synthetic Peptide within Human RNF2 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q99496

Specificity

Affinity purified Anti-RING1B antibody is directed against human RING1B protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RING1B homologues from other sources has not been determined.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

style

left-column

Product details

RING1B interacts with CP2, a mammalian member of the grainyhead-like family of transcription factors, in vitro and in vivo. The functional consequence of this interaction is repression of CP2-dependent transcription.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified Anti-RING1B antibody is directed against human RING1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RING2 also known as RING1B or RNF2 is a protein that functions as a member of the Polycomb Repressive Complex 1 (PRC1). This protein contains a RING-finger domain facilitating ubiquitin ligase activity. It has a molecular mass of approximately 38 kDa. RING2/RNF2/RING1B is expressed in various tissues including the brain heart and lungs indicating its fundamental role across different biological systems.

Biological function summary

RING2/RNF2/RING1B regulates gene expression by modifying chromatin structure especially through monoubiquitination of histone H2A at lysine 119. This action occurs within the Polycomb Repressive Complex 1 a multiprotein structure important for maintaining the transcriptional repression of target genes. It influences cell cycle progression and development by silencing genes involved in proliferation and differentiation.

Pathways

RING2/RNF2/RING1B's role connects to the Polycomb group (PcG) pathway and the Wnt signaling pathway. Within these pathways it interacts with proteins like BMI1 and EZH2 which are other components of polycomb complexes. These interactions help modulate chromatin dynamics and control the expression of several genes involved in cell identity and development.

Dysfunction or aberrant expression of RING2/RNF2/RING1B has links to cancer and neurological disorders. Its relationship with proteins like CBX8 ties it to pathways of oncogenesis where it can influence tumorigenesis through misregulation of gene silencing. Furthermore changes in RING2/RNF2/RING1B expression or function may contribute to developmental disorders associated with improper neuronal development.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation (PubMed : 15386022, PubMed : 16359901, PubMed : 21772249, PubMed : 25355358, PubMed : 25519132, PubMed : 26151332, PubMed : 33864376). H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation (By similarity). Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed : 16359901, PubMed : 26151332). PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed : 26151332). E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed : 21772249). Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (Probable). Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (By similarity). Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation (By similarity).

See full target information RNF2

style

left-column

Publications (25)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed34994686

2022

PRC1 sustains the integrity of neural fate in the absence of PRC2 function.

Applications

Unspecified application

Species

Unspecified reactive species

Ayana Sawai,Sarah Pfennig,Milica Bulajić,Alexander Miller,Alireza Khodadadi-Jamayran,Esteban O Mazzoni,Jeremy S Dasen

Journal of virology 95: PubMed33692209

2021

KDM2B Overexpression Facilitates Lytic KSHV Infection by Inducing AP-1 Activity Through Interaction with the SCF E3 Ubiquitin Ligase Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Nenavath Gopal Naik,See-Chi Lee,Juan D Alonso,Zsolt Toth

Genes & development 33:799-813 PubMed31171700

2019

Phase separation of Polycomb-repressive complex 1 is governed by a charged disordered region of CBX2.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron J Plys,Christopher P Davis,Jongmin Kim,Gizem Rizki,Madeline M Keenen,Sharon K Marr,Robert E Kingston

Journal of virology 93: PubMed30541837

2018

Genome-Wide Identification of Direct RTA Targets Reveals Key Host Factors for Kaposi's Sarcoma-Associated Herpesvirus Lytic Reactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Bernadett Papp,Naeem Motlagh,Richard J Smindak,Seung Jin Jang,Aria Sharma,Juan D Alonso,Zsolt Toth

Cell 174:172-186.e21 PubMed29958106

2018

CBFβ-SMMHC Inhibition Triggers Apoptosis by Disrupting MYC Chromatin Dynamics in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

John Anto Pulikkan,Mahesh Hegde,Hafiz Mohd Ahmad,Houda Belaghzal,Anuradha Illendula,Jun Yu,Kelsey O'Hagan,Jianhong Ou,Carsten Muller-Tidow,Scot A Wolfe,Lihua Julie Zhu,Job Dekker,John Hackett Bushweller,Lucio Hernán Castilla

Cell discovery 4:21 PubMed29736258

2018

Mtf2-PRC2 control of canonical Wnt signaling is required for definitive erythropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Janet L Manias Rothberg,Harinad B Maganti,Hani Jrade,Christopher J Porter,Gareth A Palidwor,Christopher Cafariello,Hannah L Battaion,Safwat T Khan,Theodore J Perkins,Robert F Paulson,Caryn Y Ito,William L Stanford

Genes & development 31:1988-2002 PubMed29070704

2017

Bivalent complexes of PRC1 with orthologs of BRD4 and MOZ/MORF target developmental genes in .

Applications

Unspecified application

Species

Unspecified reactive species

Hyuckjoon Kang,Youngsook L Jung,Kyle A McElroy,Barry M Zee,Heather A Wallace,Jessica L Woolnough,Peter J Park,Mitzi I Kuroda

Oncotarget 8:103731-103743 PubMed29262596

2017

The polycomb group protein BMI-1 inhibitor PTC-209 is a potent anti-myeloma agent alone or in combination with epigenetic inhibitors targeting EZH2 and the BET bromodomains.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Alzrigat,Alba Atienza Párraga,Muntasir Mamun Majumder,Anqi Ma,Jian Jin,Anders Österborg,Hareth Nahi,Kenneth Nilsson,Caroline A Heckman,Fredrik Öberg,Antonia Kalushkova,Helena Jernberg-Wiklund

Molecular cell 68:61-75.e5 PubMed28943310

2017

ASF1a Promotes Non-homologous End Joining Repair by Facilitating Phosphorylation of MDC1 by ATM at Double-Strand Breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Yong Lee,Jun-Sub Im,Etsuko Shibata,Anindya Dutta

Virology 512:25-33 PubMed28898712

2017

Inhibition of the lytic cycle of Kaposi's sarcoma-associated herpesvirus by cohesin factors following de novo infection.

Applications

Unspecified application

Species

Unspecified reactive species

Zsolt Toth,Richard J Smindak,Bernadett Papp

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RING2 / RING1B / RNF2 antibody

Western blot - Anti-RING2 / RING1B / RNF2 antibody (AB3832)

Western blot - Anti-RING2 / RING1B / RNF2 antibody (AB3832)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

Product promise