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AB88485

Anti-RIO2 antibody

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(2 Publications)

Rabbit Polyclonal RIO2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RIOK2 aa 250-350.

View Alternative Names

RIO2, RIOK2, Serine/threonine-protein kinase RIO2, RIO kinase 2

2 Images
Immunoprecipitation - Anti-RIO2 antibody (AB88485)
  • IP

Unknown

Immunoprecipitation - Anti-RIO2 antibody (AB88485)

3 μg ab88485 were used to immunoprecipitate 1 mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88485 at 1 μg/ml.

All lanes:

Immunoprecipitation - Anti-RIO2 antibody (ab88485)

Predicted band size: 63 kDa

false

Western blot - Anti-RIO2 antibody (AB88485)
  • WB

Unknown

Western blot - Anti-RIO2 antibody (AB88485)

All lanes:

Western blot - Anti-RIO2 antibody (ab88485) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human RIOK2 aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q9BVS4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebra finch": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIO2 also known as RIO kinase 2 belongs to the serine/threonine-protein kinase family. This protein has a molecular weight of approximately 67 kDa. RIO2 is widely expressed across various tissues being particularly prevalent in rapidly dividing cells. It functions as an ATP-dependent kinase essential for proper biological processes that involve cellular growth and proliferation. RIO2 works in the pre-rRNA processing and ribosome biogenesis which are important for maintaining cellular function.
Biological function summary

The protein facilitates ribosome assembly showing importance in the maturation of the 40S ribosomal subunit. RIO2 operates as part of a ribosomal RNA processing complex interacting with several other proteins involved in ribosome biogenesis. By ensuring the correct processing and assembly of rRNA RIO2 supports efficient protein synthesis which is fundamental for cell survival and growth. Its role in ribosome assembly highlights its importance in sustaining cellular functions.

Pathways

RIO2 participates actively in ribosome biogenesis and interacts with various ribosomal proteins and processing factors. It associates with well-known pathways such as the biogenesis of the small ribosomal subunit and the RNA transport pathway. In these processes RIO2 functions alongside proteins like NOP14 and ENP1 assisting in the sequential steps of the ribosome assembly line. These interactions ensure that cells maintain their protein synthesis capability under different physiological states.

RIO2 has connections to cancer and ribosomopathy. Aberrant activity or expression of RIO2 is observed in several cancers where its role in ribosome assembly is disrupted leading to uncontrolled cell growth. RIO2 also interacts with proteins involved in cancer pathways such as MYC which can further drive tumorigenesis. In ribosomopathies mutations or dysregulation of RIO2 affect ribosomal function and lead to diseases with growth and developmental defects. Addressing RIO2 abnormalities might offer therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in export of the 40S pre-ribosome particles (pre-40S) from the nucleus to the cytoplasm. Its kinase activity is required for the release of NOB1, PNO1 and LTV1 from the late pre-40S and the processing of 18S-E pre-rRNA to the mature 18S rRNA (PubMed : 19564402). Regulates the timing of the metaphase-anaphase transition during mitotic progression, and its phosphorylation, most likely by PLK1, regulates this function (PubMed : 21880710).
See full target information RIOK2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 83:4095-4111 PubMed37729426

2023

Genome-Wide CRISPR Screens Identify Multiple Synthetic Lethal Targets That Enhance KRASG12C Inhibitor Efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Suman Mukhopadhyay,Hsin-Yi Huang,Ziyan Lin,Michela Ranieri,Shuai Li,Soumyadip Sahu,Yingzhuo Liu,Yi Ban,Kayla Guidry,Hai Hu,Alfonso Lopez,Fiona Sherman,Yi Jer Tan,Yeuan Ting Lee,Amanda P Armstrong,Igor Dolgalev,Priyanka Sahu,Tinghu Zhang,Wenchao Lu,Nathanael S Gray,James G Christensen,Tracy T Tang,Vamsidhar Velcheti,Alireza Khodadadi-Jamayran,Kwok-Kin Wong,Benjamin G Neel

EBioMedicine 20:79-97 PubMed28499923

2017

The Atypical Kinase RIOK1 Promotes Tumor Growth and Invasive Behavior.

Applications

WB

Species

Human

Florian Weinberg,Nadine Reischmann,Lisa Fauth,Sanaz Taromi,Justin Mastroianni,Martin Köhler,Sebastian Halbach,Andrea C Becker,Niantao Deng,Tatjana Schmitz,Franziska Maria Uhl,Nicola Herbener,Bianca Riedel,Fabian Beier,Alexander Swarbrick,Silke Lassmann,Jörn Dengjel,Robert Zeiser,Tilman Brummer
View all publications
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Product promise

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