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AB254723

Anti-RIOK1/RIO1 antibody

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Rabbit Polyclonal RIOK1/RIO1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RIOK1 aa 50-200.

View Alternative Names

RIO1, RIOK1, Serine/threonine-protein kinase RIO1, RIO kinase 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIOK1/RIO1 antibody (AB254723)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIOK1/RIO1 antibody (AB254723)

Paraffin-embedded human skeletal muscle tissue stained for RIOK1/RIO1 using ab254723 at 1/200 dilution in immunohistochemical analysis. Low expression as expected.

Immunocytochemistry/ Immunofluorescence - Anti-RIOK1/RIO1 antibody (AB254723)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RIOK1/RIO1 antibody (AB254723)

PFA fixed, Triton X-100 permeabilized PC-3 (Human prostate adenocarcinoma cell line) cells labeling RIOK1/RIO1 using ab254723 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIOK1/RIO1 antibody (AB254723)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIOK1/RIO1 antibody (AB254723)

Paraffin-embedded human testis tissue stained for RIOK1/RIO1 using ab254723 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RIOK1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BRS2

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AB164432

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIOK1 also known as RIO1 is an atypical serine/threonine kinase with a molecular mass of approximately 67 kDa. It plays a role in processing small ribosomal subunit biogenesis. The protein is expressed in various tissues including liver and heart. Unlike typical kinases RIOK1 does not rely on the classical ATP-dependent mechanism for its kinase activity. It participates in the phosphorylation of some proteins which is necessary for ribosome maturation.
Biological function summary

The kinase RIOK1/RIO1 is part of the ribosome biogenesis pathway which is essential for protein synthesis. It forms part of a larger dynamic complex that assists in the maturation and transport of ribosomal components from the nucleus to the cytoplasm. RIOK1 plays a role in the final steps of 18S rRNA processing helping in the maturation of the 40S ribosomal subunit. This function makes it important in cellular growth and proliferation processes.

Pathways

The kinase RIOK1/RIO1 is involved in the ribosome biogenesis pathway and the associated cell cycle progression. It interacts with several proteins like NOB1 and ENP1 which are important for small subunit maturation. The efficient function of these pathways is necessary for normal cellular function and division. These partnerships ensure the necessary quality control of new ribosome parts as they are built highlighting its critical role in maintaining cellular homeostasis.

Alterations in RIOK1/RIO1 expression have been connected to cancers and neurological disorders. Aberrant expression levels contribute to abnormal cell growth in various cancer types implicating its role in tumorigenesis. Furthermore the dysregulation of RIOK1 can influence the activity of proteins such as p53 which is an important player in managing cellular stress responses and apoptosis. This association suggests potential as a biomarker for certain oncological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed : 22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (PubMed : 21081503).
See full target information RIOK1
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Product promise

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