AB88496

Anti-RIOK1/RIO1 antibody

Name: Anti-RIOK1/RIO1 antibody (ab88496) | Abcam
Brand: Abcam Limited
SKU: AB88496
Price: 525 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal RIOK1/RIO1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RIOK1.

View Alternative Names

RIO1, RIOK1, Serine/threonine-protein kinase RIO1, RIO kinase 1

2 Images

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Immunoprecipitation - Anti-RIOK1/RIO1 antibody (AB88496)

3ug ab88496 were used to immunoprecipitate 1mg HeLa whole cell lysate. 20% of immunoprecipitate was loaded on gel and probed with ab88496 at 0.4ug/ml.
Detection : chemoluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-RIOK1/RIO1 antibody (ab88496)

Predicted band size: 66 kDa

false

Unknown

Western blot - Anti-RIOK1/RIO1 antibody (AB88496)

All lanes:

Western blot - Anti-RIOK1/RIO1 antibody (ab88496) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 66 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RIOK1. The exact immunogen used to generate this antibody is proprietary information.

Q9BRS2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIOK1 also known as RIO1 is an atypical serine/threonine kinase with a molecular mass of approximately 67 kDa. It plays a role in processing small ribosomal subunit biogenesis. The protein is expressed in various tissues including liver and heart. Unlike typical kinases RIOK1 does not rely on the classical ATP-dependent mechanism for its kinase activity. It participates in the phosphorylation of some proteins which is necessary for ribosome maturation.

Biological function summary

The kinase RIOK1/RIO1 is part of the ribosome biogenesis pathway which is essential for protein synthesis. It forms part of a larger dynamic complex that assists in the maturation and transport of ribosomal components from the nucleus to the cytoplasm. RIOK1 plays a role in the final steps of 18S rRNA processing helping in the maturation of the 40S ribosomal subunit. This function makes it important in cellular growth and proliferation processes.

Pathways

The kinase RIOK1/RIO1 is involved in the ribosome biogenesis pathway and the associated cell cycle progression. It interacts with several proteins like NOB1 and ENP1 which are important for small subunit maturation. The efficient function of these pathways is necessary for normal cellular function and division. These partnerships ensure the necessary quality control of new ribosome parts as they are built highlighting its critical role in maintaining cellular homeostasis.

Alterations in RIOK1/RIO1 expression have been connected to cancers and neurological disorders. Aberrant expression levels contribute to abnormal cell growth in various cancer types implicating its role in tumorigenesis. Furthermore the dysregulation of RIOK1 can influence the activity of proteins such as p53 which is an important player in managing cellular stress responses and apoptosis. This association suggests potential as a biomarker for certain oncological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor (PubMed : 22072790). The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation (PubMed : 21081503).

See full target information RIOK1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 14:e70597 PubMed39865406

2025

RIOK1: A Novel Oncogenic Driver in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Ruan,Tianyu Shang,Sijia Zhang,Wenhong Ru,Yuefeng Yang,Yi Shen

The American journal of pathology 193:1284-1297 PubMed37301535

2023

The Oncogenic Protein Kinase/ATPase RIOK1 Is Up-Regulated via the c-myc/E2F Transcription Factor Axis in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Handle,Martin Puhr,Martina Gruber,Chiara Andolfi,Georg Schäfer,Helmut Klocker,Johannes Haybaeck,Peter De Wulf,Zoran Culig

eLife 8: PubMed31099754

2019

KLK3/PSA and cathepsin D activate VEGF-C and VEGF-D.

Applications

Unspecified application

Species

Unspecified reactive species

Sawan Kumar Jha,Khushbu Rauniyar,Ewa Chronowska,Kenny Mattonet,Eunice Wairimu Maina,Hannu Koistinen,Ulf-Håkan Stenman,Kari Alitalo,Michael Jeltsch

Oncotarget 9:28625-28637 PubMed29983885

2018

RIOK1 kinase activity is required for cell survival irrespective of status.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra Hörmann,Barbara Hopfgartner,Thomas Köcher,Maja Corcokovic,Teresa Krammer,Christoph Reiser,Gerd Bader,Junwei Shi,Katharina Ehrenhöfer,Simon Wöhrle,Norbert Schweifer,Christopher R Vakoc,Norbert Kraut,Mark Pearson,Mark Petronczki,Ralph A Neumüller

EBioMedicine 20:79-97 PubMed28499923

2017

The Atypical Kinase RIOK1 Promotes Tumor Growth and Invasive Behavior.

Applications

Species

Human

Florian Weinberg,Nadine Reischmann,Lisa Fauth,Sanaz Taromi,Justin Mastroianni,Martin Köhler,Sebastian Halbach,Andrea C Becker,Niantao Deng,Tatjana Schmitz,Franziska Maria Uhl,Nicola Herbener,Bianca Riedel,Fabian Beier,Alexander Swarbrick,Silke Lassmann,Jörn Dengjel,Robert Zeiser,Tilman Brummer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RIOK1/RIO1 antibody

Immunoprecipitation - Anti-RIOK1/RIO1 antibody (AB88496)

Western blot - Anti-RIOK1/RIO1 antibody (AB88496)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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