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AB202985

Anti-RIP antibody [EPR19697]

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(13 Publications)

Rabbit Recombinant Monoclonal RIP antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse samples. Cited in 13 publications.

View Alternative Names

Rinp, Rip, Ripk1, Receptor-interacting serine/threonine-protein kinase 1, Cell death protein RIP, Receptor-interacting protein 1, RIP-1

6 Images
Flow Cytometry (Intracellular) - Anti-RIP antibody [EPR19697] (AB202985)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RIP antibody [EPR19697] (AB202985)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling RIPwith ab202985 at 1/500 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti Rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.

Flow Cytometry (Intracellular) - Anti-RIP antibody [EPR19697] (AB202985)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RIP antibody [EPR19697] (AB202985)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling RIPwith ab202985 at 1/500 dilution (red) compared with a Rabbit IgG,monoclonal[EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti Rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody [EPR19697] (AB202985)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody [EPR19697] (AB202985)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling RIP with ab202985 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The results show cytoplasmic staining on NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202985 at 1/200 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody [EPR19697] (AB202985)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RIP antibody [EPR19697] (AB202985)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling RIP with ab202985 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The results show cytoplasmic staining on RAW 264.7 cells. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab202985 at 1/200 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

Immunoprecipitation - Anti-RIP antibody [EPR19697] (AB202985)
  • IP

Lab

Immunoprecipitation - Anti-RIP antibody [EPR19697] (AB202985)

ab202985 Immunoprecipitating RIP in mouse embryo whole cell lysate. 2μg of capture antibody per 0.35mg of lysate was used. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/1000 and VeriBlot for IP Detection Reagent (HRP) ab131366 was used for detection of 1/1000.

Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo) whole cell lysate
Lane 3 : Rabbit monoclonal IgG ab172730 instead of ab202985 in NIH/3T3 (mouse embryo) whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RIP antibody [EPR19697] (ab202985)

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 3s

Western blot - Anti-RIP antibody [EPR19697] (AB202985)
  • WB

Supplier Data

Western blot - Anti-RIP antibody [EPR19697] (AB202985)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 8 seconds; Lane 2 : 15 seconds; Lane 3-5 : 3 minutes.

The molecular weight observed is consistent with what has been described in the literatures (PMID : 15314284 & 10521396).

All lanes:

Western blot - Anti-RIP antibody [EPR19697] (ab202985) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

Mouse brain lysate at 10 µg

Lane 4:

Mouse heart lysate at 10 µg

Lane 5:

Mouse kidney lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 75 kDa

Observed band size: 44 kDa,75 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19697

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB, ICC/IF, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIP also known as Receptor-Interacting Protein or RIPK1 is a serine/threonine-protein kinase with a mass of approximately 74 kDa. It plays an important role in cell death and survival signaling pathways. RIP is expressed ubiquitously across various tissues indicating its importance in many cellular functions. The protein contains a kinase domain an intermediate domain for protein-protein interactions and a death domain which facilitates its involvement in apoptotic signaling processes.
Biological function summary

Receptor-Interacting Protein Kinase 1 (RIPK1) participates in regulating both necroptosis and apoptosis distinguishing itself as an important mediator in cell death mechanisms. As part of the necrosome complex which includes RIPK3 and MLKL RIPK1 functions in necroptosis—a programmed form of necrosis. This characteristic involvement shows its dual role in maintaining cell fate decisions making it an integral part of immune response and inflammation control.

Pathways

RIPK1 strongly associates with the TNF signaling pathway and NF-kB pathway. Its interaction with TNF receptor 1 (TNFR1) and consequent involvement with TRADD and TRAF2 mediates the signal transduction necessary for the activation of NF-kB leading to transcription of genes involved in survival and inflammation. This connection illustrates its capability to switch between promoting cell survival through NF-kB and facilitating cell death via necroptosis or apoptosis depending on cellular context and cues.

RIPK1 plays a significant role in conditions such as inflammatory diseases and neurodegenerative disorders. Its overactivation results in excessive cell death implicated in inflammatory conditions; necrostatin a necroptosis inhibitor targets RIPK1 to potentially mitigate this damage. Furthermore RIPK1's dysregulation links to Alzheimer's disease where it can interact with components like RIPK3 to exacerbate neurodegenerative processes. This relationship underlines the potential of targeting RIPK1 therapeutically to manage inflammation and neurodegeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways (PubMed : 24557836, PubMed : 24813849, PubMed : 24813850, PubMed : 27819681, PubMed : 28842570, PubMed : 31511692, PubMed : 31827280, PubMed : 31827281, PubMed : 33397971). Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival (PubMed : 24557836, PubMed : 24813849, PubMed : 24813850, PubMed : 28842570, PubMed : 31519886, PubMed : 31519887). Has kinase-independent scaffold functions : upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway (PubMed : 31519886, PubMed : 31519887). Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death : upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis (PubMed : 27819681, PubMed : 27819682, PubMed : 28842570, PubMed : 29440439, PubMed : 30988283, PubMed : 31519886, PubMed : 31519887). RIPK1 is required to limit CASP8-dependent TNFR1-induced apoptosis (PubMed : 24557836, PubMed : 24813849, PubMed : 24813850). In normal conditions, RIPK1 acts as an inhibitor of RIPK3-dependent necroptosis, a process mediated by RIPK3 component of complex IIb, which catalyzes phosphorylation of MLKL upon induction by ZBP1 (PubMed : 24557836, PubMed : 27819681, PubMed : 27819682, PubMed : 31358656). Inhibits RIPK3-mediated necroptosis via FADD-mediated recruitment of CASP8, which cleaves RIPK1 and limits TNF-induced necroptosis (PubMed : 31358656). Required to inhibit apoptosis and necroptosis during embryonic development : acts by preventing the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8 (PubMed : 30185824, PubMed : 30867408). In addition to apoptosis and necroptosis, also involved in inflammatory response by promoting transcriptional production of pro-inflammatory cytokines, such as interleukin-6 (IL6) (PubMed : 31827280, PubMed : 31827281). Phosphorylates RIPK3 : RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (By similarity). Phosphorylates DAB2IP at 'Ser-728' in a TNF-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade (By similarity). Required for ZBP1-induced NF-kappa-B activation in response to DNA damage (PubMed : 12654725, PubMed : 19590578).
See full target information Ripk1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 22:427 PubMed39223674

2024

RIPK1 inhibition mitigates neuroinflammation and rescues depressive-like behaviors in a mouse model of LPS-induced depression.

Applications

Unspecified application

Species

Unspecified reactive species

Qichao Gong,Tahir Ali,Yue Hu,Ruyan Gao,Shengnan Mou,Yanhua Luo,Canyu Yang,Axiang Li,Tao Li,Liang Liang Hao,Liufang He,Xiaoming Yu,Shupeng Li

Aging 15:13176-13193 PubMed37988198

2023

Investigating EGF and PAG1 as necroptosis-related biomarkers for diabetic nephropathy: an and validation study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuejun Wang,Linlin Zhang,Zhiping Peng

International journal of biological sciences 19:4709-4725 PubMed37781516

2023

Loss of DDRGK1 impairs IRE1α UFMylation in spondyloepiphyseal dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Yang,Tangjun Zhou,Xin Wang,Ying Xia,Xiankun Cao,Xiaofei Cheng,Yu Cao,Peixiang Ma,Hui Ma,An Qin,Jie Zhao

Brain sciences 13: PubMed37239205

2023

RIPK1-Induced A1 Reactive Astrocytes in Brain in MPTP-Treated Murine Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chenmeng Qiao,Guyu Niu,Weijiang Zhao,Wei Quan,Yu Zhou,Meixuan Zhang,Ting Li,Shengyang Zhou,Wenyan Huang,Liping Zhao,Jian Wu,Chun Cui,Yanqin Shen

Oxidative medicine and cellular longevity 2022:4499219 PubMed35927992

2022

Dopamine D2 Receptor Signaling Attenuates Acinar Cell Necroptosis in Acute Pancreatitis through the Cathepsin B/TFAM/ROS Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zengkai Wu,Xiao Han,Jingpiao Bao,Bin Li,Jie Shen,Pengli Song,Qi Peng,Xingpeng Wang,Guoyong Hu

Molecular neurobiology 59:5734-5749 PubMed35781632

2022

Astrocyte-Derived TNF-α-Activated Platelets Promote Cerebral Ischemia/Reperfusion Injury by Regulating the RIP1/RIP3/AKT Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Li,Dengping Liu,Jiaqi Xu,Jun Zha,Chen Wang,Jianzhong An,Zhanli Xie,Shigang Qiao

Cancer cell international 21:437 PubMed34416900

2021

Long non-coding RNA NEAT1 facilitates the growth, migration, and invasion of ovarian cancer cells via the let-7 g/MEST/ATGL axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Yin,Yu Wang

Nutrition & diabetes 11:23 PubMed34168108

2021

MyD88 determines the protective effects of fish oil and perilla oil against metabolic disorders and inflammation in adipose tissue from mice fed a high-fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Mingyuan Hu,Hangju Zhu,Chao Yang,Hui Xia,Xian Yang,Ligang Yang,Guiju Sun

IUBMB life 72:1960-1975 PubMed32726517

2020

Long non-coding RNA LINC01207 promotes prostate cancer progression by downregulating microRNA-1972 and upregulating LIM and SH3 protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Sugui Wang,Jianguo Qiu,Liping Wang,Ziyu Wu,Xianyun Zhang,Qiang Li,Fujin Jiang

Disease models & mechanisms 13: PubMed31953345

2020

Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Colijn,Vijay Muthukumar,Jun Xie,Siqi Gao,Courtney T Griffin
View all publications
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