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AB53740

Anti-RIP140 antibody [2656C6a]

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(2 Publications)

Mouse Monoclonal RIP140 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Nuclear receptor-interacting protein 1.

View Alternative Names

Nuclear receptor-interacting protein 1, Nuclear factor RIP140, Receptor-interacting protein 140, NRIP1

1 Images
Western blot - Anti-RIP140 antibody [2656C6a] (AB53740)
  • WB

Unknown

Western blot - Anti-RIP140 antibody [2656C6a] (AB53740)

The band size does not correspond to the predicted protein size, as the recombinant fragment immunogen was used for the test lane

All lanes:

Western blot - Anti-RIP140 antibody [2656C6a] (ab53740)

All lanes:

Immunizing recombinant protein

Predicted band size: 127 kDa

Observed band size: 41 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2656C6a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Nuclear receptor-interacting protein 1. The exact immunogen used to generate this antibody is proprietary information.

P48552

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIP140 also known as receptor-interacting protein 140 and NRIP1 is a protein that acts as a transcriptional corepressor. It has a molecular mass of approximately 115 kDa. RIP140 is found in various tissues with high levels of expression observed in the ovary testes and adipose tissue. This protein interacts with nuclear receptors and influences gene expression by recruiting chromatin-modifying enzymes.
Biological function summary

RIP140 functions in regulating energy homeostasis and reproductive processes. It plays an important role in the repression of genes involved in metabolism and fat storage. RIP140 does not act alone but is part of transcriptional complexes that modulate the activity of multiple facets of gene expression. Its ability to interact with histone deacetylases and other corepressors enhances its regulatory capacity.

Pathways

RIP140 is involved in pathways that govern glucose and lipid metabolism. It interacts notably with PPAR (peroxisome proliferator-activated receptors) and LXR (liver X receptors) both important in lipid metabolism. Energy balance influences genes that RIP140 represses or activates positioning it as a central player in metabolic regulation.

RIP140's role becomes apparent in obesity and type 2 diabetes. Abnormal expression or function of RIP140 affects metabolic pathways contributing to these conditions. The protein also interacts with PGC-1α an important regulator of mitochondrial biogenesis linking it to metabolic health and disease states. Such interactions highlight the protein's significance in maintaining normal physiological balance and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. Positive regulator of the circadian clock gene expression : stimulates transcription of BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC. Involved in the regulation of ovarian function (By similarity). Plays a role in renal development (PubMed : 28381549).
See full target information Nuclear receptor-interacting protein 1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

European cells & materials 40:21-37 PubMed32672345

2020

Modelling the catabolic environment of the moderately degenerated disc with a caprine ex vivo loaded disc culture system.

Applications

Unspecified application

Species

Unspecified reactive species

C Me Rustenburg,J W Snuggs,K S Emanuel,A Thorpe,C Sammon,C L Le Maitre,T H Smit

Neoplasma 65:881-887 PubMed29940757

2018

MicroRNA-548-3p and MicroRNA-576-5p enhance the migration and invasion of esophageal squamous cell carcinoma cells via NRIP1 down-regulation.

Applications

Unspecified application

Species

Unspecified reactive species

X F Ni,L H Zhao,G Li,M Hou,M Su,C L Zou,X Deng
View all publications
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Product promise

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