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AB42126

Anti-RIP140 antibody

4

(1 Review)

|

(40 Publications)

Rabbit Polyclonal RIP140 antibody. Suitable for IP, ChIP, WB, IHC-P, ICC/IF, IHC-Fr, IHC (PFA fixed) and reacts with Mouse, Rat, Human samples. Cited in 40 publications. Immunogen corresponding to Synthetic Peptide within Human Nuclear receptor-interacting protein 1.

View Alternative Names

Nuclear receptor-interacting protein 1, Nuclear factor RIP140, Receptor-interacting protein 140, NRIP1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP140 antibody (AB42126)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP140 antibody (AB42126)

ab42126 (4µg/ml) staining RIP140 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the nuclei of the intestinal glands.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-RIP140 antibody (AB42126)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RIP140 antibody (AB42126)

ICC/IF image of ab42126 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42126, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-P, IHC-Fr, IP, ChIP, IHC (PFA fixed), ICC/IF

applications

Immunogen

Synthetic Peptide within Human Nuclear receptor-interacting protein 1. The exact immunogen used to generate this antibody is proprietary information.

P48552

Specificity

ab42126 recognises the C terminal domain of RIP140

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 - 7.8 Constituents: 69.5% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIP140 also known as receptor-interacting protein 140 and NRIP1 is a protein that acts as a transcriptional corepressor. It has a molecular mass of approximately 115 kDa. RIP140 is found in various tissues with high levels of expression observed in the ovary testes and adipose tissue. This protein interacts with nuclear receptors and influences gene expression by recruiting chromatin-modifying enzymes.
Biological function summary

RIP140 functions in regulating energy homeostasis and reproductive processes. It plays an important role in the repression of genes involved in metabolism and fat storage. RIP140 does not act alone but is part of transcriptional complexes that modulate the activity of multiple facets of gene expression. Its ability to interact with histone deacetylases and other corepressors enhances its regulatory capacity.

Pathways

RIP140 is involved in pathways that govern glucose and lipid metabolism. It interacts notably with PPAR (peroxisome proliferator-activated receptors) and LXR (liver X receptors) both important in lipid metabolism. Energy balance influences genes that RIP140 represses or activates positioning it as a central player in metabolic regulation.

RIP140's role becomes apparent in obesity and type 2 diabetes. Abnormal expression or function of RIP140 affects metabolic pathways contributing to these conditions. The protein also interacts with PGC-1α an important regulator of mitochondrial biogenesis linking it to metabolic health and disease states. Such interactions highlight the protein's significance in maintaining normal physiological balance and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Modulates transcriptional activation by steroid receptors such as NR3C1, NR3C2 and ESR1. Also modulates transcriptional repression by nuclear hormone receptors. Positive regulator of the circadian clock gene expression : stimulates transcription of BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC. Involved in the regulation of ovarian function (By similarity). Plays a role in renal development (PubMed : 28381549).
See full target information Nuclear receptor-interacting protein 1
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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:22592 PubMed40595878

2025

LncRNAs regulates cell death in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ping'an Zou,Zhiwei Tao,Zhengxu Yang,Tao Xiong,Zhi Deng,Qincan Chen,Li Niu

Iranian journal of basic medical sciences 28:477-485 PubMed39968093

2025

SIRT5 prevents mitochondrial dysfunction and cardiac hypertrophy induced by RIP140.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Liang,Yi Huang,Qiujuan Wang,Ye Hong,Honghui Zhen,Yanfang Chen

Nature communications 15:1285 PubMed38346993

2024

The transcriptional regulatory network modulating human trophoblast stem cells to extravillous trophoblast differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Mijeong Kim,Yu Jin Jang,Muyoung Lee,Qingqing Guo,Albert J Son,Nikita A Kakkad,Abigail B Roland,Bum-Kyu Lee,Jonghwan Kim

International journal of molecular sciences 24: PubMed37834141

2023

Involvement of IL-1β-Mediated Necroptosis in Neurodevelopment Impairment after Neonatal Sepsis in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Liao,Qing Zhu,Han Huang

International journal of molecular sciences 23: PubMed35806424

2022

The Transcription Coregulator RIP140 Inhibits Cancer Cell Proliferation by Targeting the Pentose Phosphate Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Valentin Jacquier,Delphine Gitenay,Vincent Cavaillès,Catherine Teyssier

Cellular and molecular life sciences : CMLS 79:270 PubMed35501580

2022

RIP140 inhibits glycolysis-dependent proliferation of breast cancer cells by regulating GLUT3 expression through transcriptional crosstalk between hypoxia induced factor and p53.

Applications

Unspecified application

Species

Unspecified reactive species

Valentin Jacquier,Delphine Gitenay,Samuel Fritsch,Sandrine Bonnet,Balázs Győrffy,Stéphan Jalaguier,Laetitia K Linares,Vincent Cavaillès,Catherine Teyssier

Nature communications 12:6931 PubMed34836963

2021

CRISPR-enhanced human adipocyte browning as cell therapy for metabolic disease.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanouela Tsagkaraki,Sarah M Nicoloro,Tiffany DeSouza,Javier Solivan-Rivera,Anand Desai,Lawrence M Lifshitz,Yuefei Shen,Mark Kelly,Adilson Guilherme,Felipe Henriques,Nadia Amrani,Raed Ibraheim,Tomas C Rodriguez,Kevin Luk,Stacy Maitland,Randall H Friedline,Lauren Tauer,Xiaodi Hu,Jason K Kim,Scot A Wolfe,Erik J Sontheimer,Silvia Corvera,Michael P Czech

Cancers 13: PubMed34503257

2021

A Truncated NRIP1 Mutant Amplifies Microsatellite Instability of Colorectal Cancer by Regulating MSH2/MSH6 Expression, and Is a Prognostic Marker of Stage III Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Pascale Palassin,Marion Lapierre,Samuel Pyrdziak,Antoine Wagner,Régine Stehle,Carole Corsini,Jacqueline Duffour,Sandrine Bonnet,Abdelhay Boulahtouf,Carmen Rodriguez,Alexandre Ho-Pun-Cheung,Evelyne Lopez-Crapez,Florence Boissière-Michot,Frédéric Bibeau,Simon Thezenas,Nabila Elarouci,Janick Selves,Jean-Sébastien Hoffmann,Paul Roepman,Thibault Mazard,Olivier Buhard,Alex Duval,Stéphan Jalaguier,Vincent Cavaillès,Audrey Castet-Nicolas

Cell communication and signaling : CCS 19:69 PubMed34193153

2021

Regulation of exosome secretion by cellular retinoic acid binding protein 1 contributes to systemic anti-inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Wei Lin,Jennifer Nhieu,Chin-Wen Wei,Yu-Lung Lin,Hiroyuki Kagechika,Li-Na Wei

Cancers 13: PubMed34206767

2021

RIP140 Represses Intestinal Paneth Cell Differentiation and Interplays with SOX9 Signaling in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Gleizes,Mouna Triki,Sandrine Bonnet,Naomi Baccari,Gabriel Jimenez-Dominguez,Aurélie Covinhes,Nelly Pirot,Philippe Blache,Rong Yuan,Balázs Győrffy,Vincent Cavaillès,Marion Lapierre
View all publications
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