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AB217549

Anti-RIP2 antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal RIP2 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RIPK2 aa 450-500 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CARDIAK, RICK, RIP2, UNQ277/PRO314/PRO34092, RIPK2, Receptor-interacting serine/threonine-protein kinase 2, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, RIP-like-interacting CLARP kinase, Receptor-interacting protein 2, Tyrosine-protein kinase RIPK2, CARD-containing IL-1 beta ICE-kinase, RIP-2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP2 antibody (AB217549)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIP2 antibody (AB217549)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat lung tissue, labeling RIP2 using ab217549 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human RIPK2 aa 450-500 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O43353

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIP2 also known as receptor-interacting serine/threonine-protein kinase 2 or RICK is a protein with roles in signal transduction. It functions mechanically as a kinase which means it adds a phosphate group to other proteins impacting their activity and stability. RIP2 weighs about 61 kDa and is mostly expressed in immune system-related tissues such as spleen thymus peripheral blood leukocytes and bone marrow. As a kinase RIP2 is an action part in the cellular response to external stimuli.
Biological function summary

RIP2 interacts with key components of the immune signaling network. It forms part of a complex with other proteins like nucleotide-binding oligomerization domain (NOD) proteins influencing immune responses. By binding to NOD1 and NOD2 RIP2 modulates inflammatory signaling pathways leading to the activation of NF-kB and the production of inflammatory cytokines. This protein helps regulate the body's defense against bacterial infections and influences inflammation processes.

Pathways

RIP2 plays an integral role in the NOD-like receptor signaling pathway and is also involved in toll-like receptor signaling. In its pathways RIP2 interacts closely with proteins like IKK complexes and the Tak1 complex orchestrating signaling cascades leading to the activation of NF-kB and MAPK pathways. These pathways are essential for instigating the immune response and managing inflammatory signals highlighting how RIP2 acts as a pivotal mediator in these biological routes.

Mutations or dysregulation of RIP2 are linked to inflammatory diseases and immune disorders such as Crohn's disease and Blau syndrome. RIP2's involvement in these conditions often involves its interaction with NOD2 influencing the improper immune response seen in these diseases. Changing RIP2 activity presents a target for developing kinase inhibitors that might help to manage inflammation and immune system disorders by modulating its signaling activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine/tyrosine-protein kinase that plays an essential role in modulation of innate and adaptive immune responses (PubMed : 14638696, PubMed : 17054981, PubMed : 21123652, PubMed : 28656966, PubMed : 9575181, PubMed : 9642260). Acts as a key effector of NOD1 and NOD2 signaling pathways : upon activation by bacterial peptidoglycans, NOD1 and NOD2 oligomerize and recruit RIPK2 via CARD-CARD domains, leading to the formation of RIPK2 filaments (PubMed : 17054981, PubMed : 17562858, PubMed : 21123652, PubMed : 22607974, PubMed : 28656966, PubMed : 29452636, PubMed : 30026309). Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3, as well as 'Met-1'-linked (linear) polyubiquitination by the LUBAC complex, becoming a scaffolding protein for downstream effectors (PubMed : 22607974, PubMed : 28545134, PubMed : 29452636, PubMed : 30026309, PubMed : 30279485, PubMed : 30478312). 'Met-1'-linked polyubiquitin chains attached to RIPK2 recruit IKBKG/NEMO, which undergoes 'Lys-63'-linked polyubiquitination in a RIPK2-dependent process (PubMed : 17562858, PubMed : 22607974, PubMed : 29452636, PubMed : 30026309). 'Lys-63'-linked polyubiquitin chains attached to RIPK2 serve as docking sites for TAB2 and TAB3 and mediate the recruitment of MAP3K7/TAK1 to IKBKG/NEMO, inducing subsequent activation of IKBKB/IKKB (PubMed : 18079694). In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis (PubMed : 18079694). The protein kinase activity is dispensable for the NOD1 and NOD2 signaling pathways (PubMed : 29452636, PubMed : 30026309). Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappa-B activation by NOD2 (PubMed : 21887730). Also involved in adaptive immunity : plays a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation (PubMed : 14638696). Plays a role in the inactivation of RHOA in response to NGFR signaling (PubMed : 26646181).
See full target information RIPK2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Genes, chromosomes & cancer 63:e70007 PubMed39535842

2024

Differential Expression of Proteins and Genes at the Tumor-Brain Interface in Invasive Meningioma.

Applications

Unspecified application

Species

Unspecified reactive species

Kornwika Senglek,Chinachote Teerapakpinyo,Nutchawan Jittapiromsak,Pakrit Jittapiromsak,Irin Lertparinyaphorn,Paul Scott Thorner,Shanop Shuangshoti
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