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AB237511

Anti-RIPX antibody

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(1 Publication)

Rabbit Polyclonal RIPX antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RUFY3 aa 200-400.

View Alternative Names

KIAA0871, RUFY3, Protein RUFY3, RUN and FYVE domain-containing protein 3, Rap2-interacting protein x, Single axon-regulated protein, RIPx, Singar

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RIPX antibody (AB237511)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RIPX antibody (AB237511)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RIPX (green) using ab237511 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

Western blot - Anti-RIPX antibody (AB237511)
  • WB

Supplier Data

Western blot - Anti-RIPX antibody (AB237511)

All lanes:

Western blot - Anti-RIPX antibody (ab237511) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 3:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 4:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RUFY3 aa 200-400. The exact immunogen used to generate this antibody is proprietary information.

Q7L099

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIPX also known as Receptor Interacting Protein X is a signaling protein with a mass of approximately 74 kDa. This protein acts as an important intermediary within various cellular mechanisms. It is expressed in numerous tissues including the spleen liver and the immune system's vital organs. RIPX possesses a death domain which plays a role in the regulation of cellular responses.
Biological function summary

RIPX has significant roles in the immune response and apoptotic processes. RIPX associates with other proteins to form complex structures that modulate cell death and survival ensuring balanced cellular environments. Through its interactions RIPX influences cytokine production and inflammation which are critical aspects of immune system regulation.

Pathways

The signaling mechanisms involving RIPX have been linked closely with the NF-κB and TNF receptor signaling pathways. RIPX acts as an important node working alongside other proteins like RIP1 and TRAF2 to propagate signals that preclude inflammation and apoptosis. Its regulatory function ensures proper communication within cells affecting the balance between cell death and survival in response to external stimuli.

RIPX has connections to inflammatory diseases such as rheumatoid arthritis and Crohn's disease. Dysregulation of RIPX activity can lead to aberrant inflammation linking it to these pathologies. RIPX interactions with proteins like TNF receptor-associated factors can exacerbate inflammatory responses highlighting a potential target for therapeutic intervention in managing chronic inflammation conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the generation of neuronal polarity formation and axon growth (By similarity). Implicated in the formation of a single axon by developing neurons (By similarity). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (By similarity). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery (PubMed : 25766321). Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (By similarity). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner (PubMed : 25766321).
See full target information RUFY3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biochemistry 479:929-940 PubMed37256445

2023

miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Dong,Jin Chen,Yadong Wang,Junfei Weng,Xingxiang Du,Xu Fang,Wenyu Liu,Tao Long,Jiaxiang You,Wensheng Wang,Xiaoping Peng
View all publications
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Product promise

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