Rabbit Polyclonal RIPX antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RUFY3 aa 200-400.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RUFY3 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q7L099
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Target data
Function
Plays a role in the generation of neuronal polarity formation and axon growth (By similarity). Implicated in the formation of a single axon by developing neurons (By similarity). May inhibit the formation of additional axons by inhibition of PI3K in minor neuronal processes (By similarity). Plays a role in the formation of F-actin-enriched protrusive structures at the cell periphery (PubMed:25766321). Plays a role in cytoskeletal organization by regulating the subcellular localization of FSCN1 and DBN1 at axonal growth cones (By similarity). Promotes gastric cancer cell migration and invasion in a PAK1-dependent manner (PubMed:25766321).
Alternative names
KIAA0871, RUFY3, Protein RUFY3, RUN and FYVE domain-containing protein 3, Rap2-interacting protein x, Single axon-regulated protein, RIPx, Singar
Recommended products
Rabbit Polyclonal RIPX antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RUFY3 aa 200-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RUFY3 aa 200-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q7L099
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity greater than 95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RIPX also known as Receptor Interacting Protein X is a signaling protein with a mass of approximately 74 kDa. This protein acts as an important intermediary within various cellular mechanisms. It is expressed in numerous tissues including the spleen liver and the immune system's vital organs. RIPX possesses a death domain which plays a role in the regulation of cellular responses.
- Biological function summary
RIPX has significant roles in the immune response and apoptotic processes. RIPX associates with other proteins to form complex structures that modulate cell death and survival ensuring balanced cellular environments. Through its interactions RIPX influences cytokine production and inflammation which are critical aspects of immune system regulation.
- Pathways
The signaling mechanisms involving RIPX have been linked closely with the NF-κB and TNF receptor signaling pathways. RIPX acts as an important node working alongside other proteins like RIP1 and TRAF2 to propagate signals that preclude inflammation and apoptosis. Its regulatory function ensures proper communication within cells affecting the balance between cell death and survival in response to external stimuli.
- Associated diseases and disorders
RIPX has connections to inflammatory diseases such as rheumatoid arthritis and Crohn's disease. Dysregulation of RIPX activity can lead to aberrant inflammation linking it to these pathologies. RIPX interactions with proteins like TNF receptor-associated factors can exacerbate inflammatory responses highlighting a potential target for therapeutic intervention in managing chronic inflammation conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-RIPX antibody (ab237511)
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RIPX (green) using ab237511 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
Western blot - Anti-RIPX antibody (ab237511)
All lanes: Western blot - Anti-RIPX antibody (ab237511) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 kDa
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