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AB154758

Anti-RISC antibody

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(1 Publication)

Rabbit Polyclonal RISC antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SCPEP1 aa 150 to C-terminus.

View Alternative Names

RISC, SCP1, MSTP034, UNQ265/PRO302, SCPEP1, Retinoid-inducible serine carboxypeptidase, Serine carboxypeptidase 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RISC antibody (AB154758)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RISC antibody (AB154758)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling RISC with ab154758 at 1/200 dilution. Lower image is merged with DNA probe.

Western blot - Anti-RISC antibody (AB154758)
  • WB

Unknown

Western blot - Anti-RISC antibody (AB154758)

10% SDS PAGE

All lanes:

Western blot - Anti-RISC antibody (ab154758) at 1/2000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 35 kDa,51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SCPEP1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9HB40

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RISC also known as the RNA-induced silencing complex functions mechanically by binding to small interfering RNA (siRNA) or microRNA (miRNA) to guide RNA silencing processes. The complex has not a fixed mass as it consists of multiple proteins and RNA molecules. RISC is typically found in the cytoplasm of eukaryotic cells where it engages in post-transcriptional gene regulation. Key components of RISC include the Argonaute protein family which plays an important role in its RNA-binding and cleavage activities.
Biological function summary

The RISC complex takes part in regulating gene expression by degrading target mRNAs or inhibiting their translation once bound to them. It is part of the RNA interference (RNAi) machinery a mechanism important for maintaining cellular homeostasis through gene expression control. The effector part of RISC recognizes and binds to complementary sequences on target mRNAs harnessing its RNA-binding capabilities to mediate gene silencing effectively. This process helps to regulate developmental timings cellular responses to stress and defense against viral infections.

Pathways

The RISC complex integrates into pathways like RNA interference and miRNA-mediated gene silencing important for controlling gene expression at the post-transcriptional level. Within these pathways RISC interacts with proteins such as Dicer which processes precursor RNA molecules into mature siRNA or miRNA that RISC then utilizes for gene silencing. By interacting with these proteins RISC facilitates an intricate network of genetic regulation essential for cellular integrity and function.

Misregulation involving the RISC complex often associates with conditions like cancer and neurodegenerative diseases. Dysregulation of miRNA pathways where RISC plays a central role can lead to cancer by allowing unchecked cell proliferation or failure to undergo apoptosis. In neurodegenerative diseases abnormalities in RISC function can affect neuronal gene expression. Proteins like Argonaute which are important components of RISC are often implicated alongside RISC in the molecular pathogenesis of these disorders highlighting the significance of proper RISC function in maintaining health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in vascular wall and kidney homeostasis.
See full target information SCPEP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Disease markers 2022:9310048 PubMed36277988

2022

lncRNA DLEU2 Accelerates Oral Cancer Progression via miR-30a-5p/RAP1B Axis to Regulate p38 MAPK Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbo Zhang,Yanchun Wang,Pu Xu,Yongxiu Du,Weiwei Guan
View all publications
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Product promise

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