JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB127041

Anti-RIT1/RIT antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal RIT1/RIT antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RIT1 aa 1-150.

View Alternative Names

RIBB, RIT, ROC1, RIT1, GTP-binding protein Rit1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIT1/RIT antibody (AB127041)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIT1/RIT antibody (AB127041)

ab127041, at a 1/250 dilution, staining RIT1/RIT in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

Western blot - Anti-RIT1/RIT antibody (AB127041)
  • WB

Unknown

Western blot - Anti-RIT1/RIT antibody (AB127041)

12% SDS PAGE

All lanes:

Western blot - Anti-RIT1/RIT antibody (ab127041) at 1/1000 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 25 kDa,31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RIT1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q92963

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIT1 also known as RIT or Ras-like without CAAX 1 is a small GTPase with a mass of approximately 21 kDa. It belongs to the Ras superfamily of proteins which are involved in signal transduction. RIT1 is widely expressed in various tissues including the brain where it plays a role in neuronal functions. Its structure and sequence share similarities with other Ras-related proteins although its regulatory mechanisms may differ.
Biological function summary

This protein regulates cell signaling pathways that control growth differentiation and survival. It does not exist alone but interacts with multiple effector proteins to exert its function. RIT1 has been implicated in protecting cells from stress-induced apoptosis suggesting its role in maintaining cell homeostasis. Researchers continue to examine how RIT1 modulates intracellular processes and its influence over cellular dynamics.

Pathways

The Ras/MAPK and PI3K/AKT signaling pathways are where RIT1 participates actively. The protein operates closely with MAP kinases which further regulate gene expression and cellular responses. In the PI3K/AKT pathway RIT1 facilitates the signaling required for cell survival and growth. Its interactions with these pathways highlight the influence it has on key regulatory proteins like AKT.

RIT1 mutations have associations with Noonan syndrome and certain cancers. Researchers have identified connections between RIT1 and other Ras pathway-related proteins such as KRAS and NRAS which are frequently mutated in similar conditions. Understanding these associations helps unravel RIT1's role in pathogenesis and highlights its value as a potential target for therapeutic interventions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. Involved in ELK1 transactivation through the Ras-MAPK signaling cascade that mediates a wide variety of cellular functions, including cell proliferation, survival, and differentiation.
See full target information RIT1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 132: PubMed30890647

2019

Nuclear actin interactome analysis links actin to KAT14 histone acetyl transferase and mRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Tiina Viita,Salla Kyheröinen,Bina Prajapati,Jori Virtanen,Mikko J Frilander,Markku Varjosalo,Maria K Vartiainen
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB53720

Anti-RIT1/RIT antibody

Immunocytochemistry/ Immunofluorescence - Anti-RIT1/RIT antibody (AB53720)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB108602

Anti-Ras antibody [EPR3255]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ras antibody [EPR3255] (AB108602)

  • 5
  • 9
Primary Antibodies

AB176570

Anti-MRas antibody [EPR12457]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-MRas antibody [EPR12457] (AB176570)

  • 0
  • 0
Primary Antibodies

AB62322

Anti-SHP2 (phospho Y542) antibody [EP508(2)Y]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-SHP2 (phospho Y542) antibody [EP508(2)Y] (AB62322)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com