AB53720

Anti-RIT1/RIT antibody

Name: Anti-RIT1/RIT antibody. Rabbit polyclonal (ab53720) | Abcam
Brand: Abcam Limited
SKU: AB53720

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(11 Publications)

Rabbit Polyclonal RIT1/RIT antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications.

View Alternative Names

RIBB, RIT, ROC1, RIT1, GTP-binding protein Rit1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RIT1/RIT antibody (AB53720)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RIT1/RIT antibody (AB53720)

ICC/IF image of ab53720 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Supplier Data

Western blot - Anti-RIT1/RIT antibody (AB53720)

All lanes:

Western blot - Anti-RIT1/RIT antibody (ab53720) at 1/500 dilution

All lanes:

Human Jurkat cells

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIT1 also known as RIT or Ras-like without CAAX 1 is a small GTPase with a mass of approximately 21 kDa. It belongs to the Ras superfamily of proteins which are involved in signal transduction. RIT1 is widely expressed in various tissues including the brain where it plays a role in neuronal functions. Its structure and sequence share similarities with other Ras-related proteins although its regulatory mechanisms may differ.

Biological function summary

This protein regulates cell signaling pathways that control growth differentiation and survival. It does not exist alone but interacts with multiple effector proteins to exert its function. RIT1 has been implicated in protecting cells from stress-induced apoptosis suggesting its role in maintaining cell homeostasis. Researchers continue to examine how RIT1 modulates intracellular processes and its influence over cellular dynamics.

Pathways

The Ras/MAPK and PI3K/AKT signaling pathways are where RIT1 participates actively. The protein operates closely with MAP kinases which further regulate gene expression and cellular responses. In the PI3K/AKT pathway RIT1 facilitates the signaling required for cell survival and growth. Its interactions with these pathways highlight the influence it has on key regulatory proteins like AKT.

RIT1 mutations have associations with Noonan syndrome and certain cancers. Researchers have identified connections between RIT1 and other Ras pathway-related proteins such as KRAS and NRAS which are frequently mutated in similar conditions. Understanding these associations helps unravel RIT1's role in pathogenesis and highlights its value as a potential target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. Involved in ELK1 transactivation through the Ras-MAPK signaling cascade that mediates a wide variety of cellular functions, including cell proliferation, survival, and differentiation.

See full target information RIT1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40085681

2025

A microglia clonal inflammatory disorder in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rocio Vicario,Stamatina Fragkogianni,Leslie Weber,Tomi Lazarov,Yang Hu,Samantha Y Hayashi,Barbara Craddock,Nicholas D Socci,Araitz Alberdi,Ann Baako,Oyku Ay,Masato Ogishi,Estibaliz Lopez-Rodrigo,Rajya Kappagantula,Agnes Viale,Christine A Iacobuzio-Donahue,Ting Zhou,Richard M Ransohoff,Richard Chesworth,Omar Abdel-Wahab,Bertrand Boisson,Olivier Elemento,Jean-Laurent Casanova,W Todd Miller,Frédéric Geissmann

Biology direct 20:19 PubMed39920793

2025

Oncogenic RIT1 mutations confer ferroptosis vulnerability in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruilan Ma,Dian Yang,Peng Wang,Ziyi Zhang,Xuehong Zhang,Jialiang Song,Han Liu,Shuyan Liu,Yingqiu Zhang,Lijuan Zou

iScience 27:110499 PubMed39161959

2024

The deubiquitinase USP9X regulates RIT1 protein abundance and oncogenic phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda K Riley,Michael Grant,Aidan Snell,Elizabeth Cromwell,Athea Vichas,Sitapriya Moorthi,Callie Rominger,Shrikar P Modukuri,Anatoly Urisman,Pau Castel,Lixin Wan,Alice H Berger

Science advances 9:eadf4766 PubMed37450595

2023

RAS-dependent RAF-MAPK hyperactivation by pathogenic RIT1 is a therapeutic target in Noonan syndrome-associated cardiac hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Cuevas-Navarro,Morgan Wagner,Richard Van,Monalisa Swain,Stephanie Mo,John Columbus,Madeline R Allison,Alice Cheng,Simon Messing,Thomas J Turbyville,Dhirendra K Simanshu,Matthew J Sale,Frank McCormick,Andrew G Stephen,Pau Castel

Open medicine (Warsaw, Poland) 18:20230700 PubMed37215053

2023

NECAB3 promotes the migration and invasion of liver cancer cells through HIF-1α/RIT1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yicheng Tian,Longjiang Shao,Qi Wang,Gan Ru,Chuntao Zhai,Lihui Zhou

Cancer discovery 12:2434-2453 PubMed35904492

2022

Impaired Proteolysis of Noncanonical RAS Proteins Drives Clonal Hematopoietic Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Chen,Rahul S Vedula,Antonio Cuevas-Navarro,Bin Lu,Simon J Hogg,Eric Wang,Salima Benbarche,Katherine Knorr,Won Jun Kim,Robert F Stanley,Hana Cho,Caroline Erickson,Michael Singer,Dan Cui,Steven Tittley,Benjamin H Durham,Tatiana S Pavletich,Elise Fiala,Michael F Walsh,Daichi Inoue,Sebastien Monette,Justin Taylor,Neal Rosen,Frank McCormick,R Coleman Lindsley,Pau Castel,Omar Abdel-Wahab

eLife 11: PubMed35467524

2022

Cross-species analysis of LZTR1 loss-of-function mutants demonstrates dependency to RIT1 orthologs.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Cuevas-Navarro,Laura Rodriguez-Muñoz,Joaquim Grego-Bessa,Alice Cheng,Katherine A Rauen,Anatoly Urisman,Frank McCormick,Gerardo Jimenez,Pau Castel

Nature communications 12:4789 PubMed34373451

2021

Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Athea Vichas,Amanda K Riley,Naomi T Nkinsi,Shriya Kamlapurkar,Phoebe C R Parrish,April Lo,Fujiko Duke,Jennifer Chen,Iris Fung,Jacqueline Watson,Matthew Rees,Austin M Gabel,James D Thomas,Robert K Bradley,John K Lee,Emily M Hatch,Marina K Baine,Natasha Rekhtman,Marc Ladanyi,Federica Piccioni,Alice H Berger

Current biology : CB 31:3915-3924.e9 PubMed34237269

2021

The RAS GTPase RIT1 compromises mitotic fidelity through spindle assembly checkpoint suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio Cuevas-Navarro,Richard Van,Alice Cheng,Anatoly Urisman,Pau Castel,Frank McCormick

Nature genetics 53:707-718 PubMed33846634

2021

Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition.

Applications

Unspecified application

Species

Unspecified reactive species

Daichi Inoue,Jacob T Polaski,Justin Taylor,Pau Castel,Sisi Chen,Susumu Kobayashi,Simon J Hogg,Yasutaka Hayashi,Jose Mario Bello Pineda,Ettaib El Marabti,Caroline Erickson,Katherine Knorr,Miki Fukumoto,Hiromi Yamazaki,Atsushi Tanaka,Chie Fukui,Sydney X Lu,Benjamin H Durham,Bo Liu,Eric Wang,Sanjoy Mehta,Daniel Zakheim,Ralph Garippa,Alex Penson,Guo-Liang Chew,Frank McCormick,Robert K Bradley,Omar Abdel-Wahab

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RIT1/RIT antibody

Immunocytochemistry/ Immunofluorescence - Anti-RIT1/RIT antibody (AB53720)

Western blot - Anti-RIT1/RIT antibody (AB53720)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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