Rabbit Polyclonal RIT1/RIT antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications.
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- Cancer discovery 12:2434-24532022Impaired Proteolysis of Noncanonical RAS Proteins Drives Clonal Hematopoietic Transformation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSisi Chen,Rahul S Vedula,Antonio Cuevas-Navarro,Bin Lu,Simon J Hogg,Eric Wang,Salima Benbarche,Katherine Knorr,Won Jun Kim,Robert F Stanley,Hana Cho,Caroline Erickson,Michael Singer,Dan Cui,Steven Tittley,Benjamin H Durham,Tatiana S Pavletich,Elise Fiala,Michael F Walsh,Daichi Inoue,Sebastien Monette,Justin Taylor,Neal Rosen,Frank McCormick,R Coleman Lindsley,Pau Castel,Omar Abdel-WahabPubMed 35904492
- eLife 11:2022Cross-species analysis of LZTR1 loss-of-function mutants demonstrates dependency to RIT1 orthologs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntonio Cuevas-Navarro,Laura Rodriguez-Muñoz,Joaquim Grego-Bessa,Alice Cheng,Katherine A Rauen,Anatoly Urisman,Frank McCormick,Gerardo Jimenez,Pau CastelPubMed 35467524
- Nature communications 12:47892021Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAthea Vichas,Amanda K Riley,Naomi T Nkinsi,Shriya Kamlapurkar,Phoebe C R Parrish,April Lo,Fujiko Duke,Jennifer Chen,Iris Fung,Jacqueline Watson,Matthew Rees,Austin M Gabel,James D Thomas,Robert K Bradley,John K Lee,Emily M Hatch,Marina K Baine,Natasha Rekhtman,Marc Ladanyi,Federica Piccioni,Alice H BergerPubMed 34373451
- Current biology : CB 31:3915-3924.e92021The RAS GTPase RIT1 compromises mitotic fidelity through spindle assembly checkpoint suppression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntonio Cuevas-Navarro,Richard Van,Alice Cheng,Anatoly Urisman,Pau Castel,Frank McCormickPubMed 34237269
- Nature genetics 53:707-7182021Minor intron retention drives clonal hematopoietic disorders and diverse cancer predisposition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaichi Inoue,Jacob T Polaski,Justin Taylor,Pau Castel,Sisi Chen,Susumu Kobayashi,Simon J Hogg,Yasutaka Hayashi,Jose Mario Bello Pineda,Ettaib El Marabti,Caroline Erickson,Katherine Knorr,Miki Fukumoto,Hiromi Yamazaki,Atsushi Tanaka,Chie Fukui,Sydney X Lu,Benjamin H Durham,Bo Liu,Eric Wang,Sanjoy Mehta,Daniel Zakheim,Ralph Garippa,Alex Penson,Guo-Liang Chew,Frank McCormick,Robert K Bradley,Omar Abdel-WahabPubMed 33846634
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Plays a crucial role in coupling NGF stimulation to the activation of both EPHB2 and MAPK14 signaling pathways and in NGF-dependent neuronal differentiation. Involved in ELK1 transactivation through the Ras-MAPK signaling cascade that mediates a wide variety of cellular functions, including cell proliferation, survival, and differentiation.
Alternative names
RIBB, RIT, ROC1, RIT1, GTP-binding protein Rit1, Ras-like protein expressed in many tissues, Ras-like without CAAX protein 1
Recommended products
Rabbit Polyclonal RIT1/RIT antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RIT1 also known as RIT or Ras-like without CAAX 1 is a small GTPase with a mass of approximately 21 kDa. It belongs to the Ras superfamily of proteins which are involved in signal transduction. RIT1 is widely expressed in various tissues including the brain where it plays a role in neuronal functions. Its structure and sequence share similarities with other Ras-related proteins although its regulatory mechanisms may differ.
- Biological function summary
This protein regulates cell signaling pathways that control growth differentiation and survival. It does not exist alone but interacts with multiple effector proteins to exert its function. RIT1 has been implicated in protecting cells from stress-induced apoptosis suggesting its role in maintaining cell homeostasis. Researchers continue to examine how RIT1 modulates intracellular processes and its influence over cellular dynamics.
- Pathways
The Ras/MAPK and PI3K/AKT signaling pathways are where RIT1 participates actively. The protein operates closely with MAP kinases which further regulate gene expression and cellular responses. In the PI3K/AKT pathway RIT1 facilitates the signaling required for cell survival and growth. Its interactions with these pathways highlight the influence it has on key regulatory proteins like AKT.
- Associated diseases and disorders
RIT1 mutations have associations with Noonan syndrome and certain cancers. Researchers have identified connections between RIT1 and other Ras pathway-related proteins such as KRAS and NRAS which are frequently mutated in similar conditions. Understanding these associations helps unravel RIT1's role in pathogenesis and highlights its value as a potential target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-RIT1/RIT antibody (ab53720)
ICC/IF image of ab53720 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53720, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-RIT1/RIT antibody (ab53720)
All lanes: Western blot - Anti-RIT1/RIT antibody (ab53720) at 1/500 dilution
All lanes: Human Jurkat cells
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