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AB204110

Anti-RIZ1 antibody

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(1 Publication)

Rabbit Polyclonal RIZ1 antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PRDM2 aa 1250-1300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KMT8, RIZ, PRDM2, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger protein RIZ

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIZ1 antibody (AB204110)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIZ1 antibody (AB204110)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat brain tissue labeling RIZ1 with ab204110 at 1/200 dilution. The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated, used at 1/200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIZ1 antibody (AB204110)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RIZ1 antibody (AB204110)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat liver tissue tissue labeling RIZ1 with ab204110 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human PRDM2 aa 1250-1300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q13029

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50-1/200.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIZ1 also known as PRDM2 is a tumor suppressor gene coding for a protein with a mass of approximately 280 kDa. This protein functions mechanically as a histone methyltransferase specifically modifying histone H3 on lysine 9. The structural component of RIZ1 includes a PR domain and a SET domain essential for its methyltransferase activity. This protein gets expressed in various tissues particularly in the brain ovary testis and skeletal muscle.
Biological function summary

The RIZ1 protein plays a significant role in transcriptional regulation and cell cycle arrest. It forms complexes with other proteins allowing it to exert its regulatory functions effectively. These complexes enable RIZ1 to bind DNA and affect chromatin structure which is critical for the transcriptional silencing of genes involved in cell proliferation. By blocking cell cycle progression RIZ1 helps maintain genomic stability ensuring that cells do not grow uncontrollably.

Pathways

RIZ1 protein influences gene expression and cellular processes by interacting with key signaling pathways including the p53 pathway and the retinoblastoma (Rb) pathway. These pathways regulate cell cycle checkpoints and apoptosis where RIZ1 assists in enhancing the tumor suppressive activities of p53 or Rb. Additionally RIZ1 interacts with proteins like MDM2 and E2F to modulate these pathways reinforcing its role in controlling cellular proliferation and survival.

RIZ1 shows strong associations with cancer notably breast cancer and hepatocellular carcinoma. Researchers have observed that RIZ1 expression levels are frequently reduced in these cancers. The interaction between RIZ1 and the retinoblastoma protein links it to these malignancies as loss or inactivation of RIZ1 mitigates its tumor-suppressive functions. This association suggests that therapies targeting pathways involving both RIZ1 and other proteins like p53 might prove beneficial in treating these cancers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
See full target information PRDM2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 40:140-151 PubMed39415627

2024

H3K27 Acetylation-Activated GLDC Accelerated the Advancement of Oral Squamous Cell Carcinoma by Suppressing the p53 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Xu,Qingfeng Xu,Haibing Yang
View all publications
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