Rabbit Monoclonal RIZ1 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human, Mouse samples.
Images
![Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--western-blot-img405664.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106)")
![Immunoprecipitation - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunoprecipitation-img406412.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106)")
![Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--western-blot-img405663.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405661.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405660.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (AB305106)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Tested | Tested | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. Binds to the macrophage-specific TPA-responsive element (MTE) of the HMOX1 (heme oxygenase 1) gene and may act as a transcriptional activator of this gene.
Alternative names
KMT8, RIZ, PRDM2, PR domain zinc finger protein 2, GATA-3-binding protein G3B, Lysine N-methyltransferase 8, MTB-ZF, MTE-binding protein, PR domain-containing protein 2, Retinoblastoma protein-interacting zinc finger protein, Zinc finger protein RIZ
Recommended products
Rabbit Monoclonal RIZ1 antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25164-34
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RIZ1 also known as PRDM2 is a tumor suppressor gene coding for a protein with a mass of approximately 280 kDa. This protein functions mechanically as a histone methyltransferase specifically modifying histone H3 on lysine 9. The structural component of RIZ1 includes a PR domain and a SET domain essential for its methyltransferase activity. This protein gets expressed in various tissues particularly in the brain ovary testis and skeletal muscle.
- Biological function summary
The RIZ1 protein plays a significant role in transcriptional regulation and cell cycle arrest. It forms complexes with other proteins allowing it to exert its regulatory functions effectively. These complexes enable RIZ1 to bind DNA and affect chromatin structure which is critical for the transcriptional silencing of genes involved in cell proliferation. By blocking cell cycle progression RIZ1 helps maintain genomic stability ensuring that cells do not grow uncontrollably.
- Pathways
RIZ1 protein influences gene expression and cellular processes by interacting with key signaling pathways including the p53 pathway and the retinoblastoma (Rb) pathway. These pathways regulate cell cycle checkpoints and apoptosis where RIZ1 assists in enhancing the tumor suppressive activities of p53 or Rb. Additionally RIZ1 interacts with proteins like MDM2 and E2F to modulate these pathways reinforcing its role in controlling cellular proliferation and survival.
- Associated diseases and disorders
RIZ1 shows strong associations with cancer notably breast cancer and hepatocellular carcinoma. Researchers have observed that RIZ1 expression levels are frequently reduced in these cancers. The interaction between RIZ1 and the retinoblastoma protein links it to these malignancies as loss or inactivation of RIZ1 mitigates its tumor-suppressive functions. This association suggests that therapies targeting pathways involving both RIZ1 and other proteins like p53 might prove beneficial in treating these cancers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--western-blot-img405664.jpg "Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)
This data was developed using 305105, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
The molecular weight observed is consistent with what has been described in the literature (PMID: 33585202).
Exposure time:
Lane 1: 26 seconds
Lane 2: 70 seconds
All lanes: Western blot - Anti-Riz1 antibody [EPR25164-34] (Anti-Riz1 antibody [EPR25164-34] ab305105) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 260 kDa
![Immunoprecipitation - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunoprecipitation-img406412.jpg "Immunoprecipitation - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
Immunoprecipitation - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)
This data was developed using Anti-Riz1 antibody [EPR25164-34] ab305105, the same antibody clone in a different buffer formulation.
Riz1 was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug with Anti-Riz1 antibody [EPR25164-34] ab305105 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Riz1 antibody [EPR25164-34] ab305105 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug
Lane 2: ABAB305105 IP in MCF7 whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Riz1 antibody [EPR25164-34] ab305105 in MCF7 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.All lanes: Immunoprecipitation - Anti-Riz1 antibody [EPR25164-34] (Anti-Riz1 antibody [EPR25164-34] ab305105) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 μg
Lane 2: MCF7 whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 260 kDa
Exposure time: 8s
![Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--western-blot-img405663.jpg "Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
Western blot - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)
This data was developed using 305105, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: The identity of the lower MW band at approximately 100 kDa is unknown.
10 seconds
Exposure time:All lanes: Western blot - Anti-Riz1 antibody [EPR25164-34] (Anti-Riz1 antibody [EPR25164-34] ab305105) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate 10 μg
Lane 2: HeLa transfected with siRNA specifically targeti PR domain zinc fier protein 2, whole cell lysate 10 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 260 kDa
Exposure time: 10s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405661.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)
This data was developed using Anti-Riz1 antibody [EPR25164-34] ab305105, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Riz1 with Anti-Riz1 antibody [EPR25164-34] ab305105 at 1/16000 (0.032 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on mouse lung. The section was incubated with Anti-Riz1 antibody [EPR25164-34] ab305105 at 4ЎгC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405660.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)
This data was developed using Anti-Riz1 antibody [EPR25164-34] ab305105, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Riz1 with Anti-Riz1 antibody [EPR25164-34] ab305105 at 1/16000 (0.032 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on human lung. The section was incubated with Anti-Riz1 antibody [EPR25164-34] ab305105 at 4ЎгC overnight. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)![Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunocytochemistry-immunofluorescence-img405659.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)
This data was developed using Anti-Riz1 antibody [EPR25164-34] ab305105, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeABilized MCF7 (human breast adenocarcinoma epithelial cellЈ© cells lABelling Riz1 with Anti-Riz1 antibody [EPR25164-34] ab305105 at 1/100 (5.09 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing nuclear staining in MCF7 cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2ug/mL dilution.![Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunocytochemistry-immunofluorescence-img405658.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)
This data was developed using Anti-Riz1 antibody [EPR25164-34] ab305105, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeABilized F9 (mouse embryonal carcinoma epithelial cell) cells lABelling Riz1 with Anti-Riz1 antibody [EPR25164-34] ab305105 at 1/100 (5.09 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing nuclear staining in F9 cell line.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor? 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2ug/mL dilution.![Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--flow-cytometry-intracellular-img405657.jpg "Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)
This data was developed using Anti-Riz1 antibody [EPR25164-34] ab305105, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized MCF7 (human breast adenocarcinoma epithelial cell) cells lABelling Riz1 with Anti-Riz1 antibody [EPR25164-34] ab305105 at 1/500 dilution (0.1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.![Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--flow-cytometry-intracellular-img405656.jpg "Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)
This data was developed using Anti-Riz1 antibody [EPR25164-34] ab305105, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeABilized F9 (mouse embryonal carcinoma epithelial cell) cells lABelling Riz1 with Anti-Riz1 antibody [EPR25164-34] ab305105 at 1/500 dilution (0.1ug) (Red) (Red) compared with a RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlABelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-RABbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.
![Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunocytochemistry-immunofluorescence-img405659.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
![Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--immunocytochemistry-immunofluorescence-img405658.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
![Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--flow-cytometry-intracellular-img405657.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
![Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106), expandable thumbnail](https://content.abcam.com/products/images/riz1-antibody-epr25164-34-bsa-and-azide-free-ab305106--flow-cytometry-intracellular-img405656.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Riz1 antibody [EPR25164-34] - BSA and Azide free (ab305106)")
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