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Rabbit Recombinant Monoclonal RKIP antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 20 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (AB76582), expandable thumbnail
  • Western blot - Anti-RKIP antibody [EPR2875Y] (AB76582), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (AB76582), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (AB76582), expandable thumbnail
  • Western blot - Anti-RKIP antibody [EPR2875Y] (AB76582), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Tested
Tested
Tested
Mouse
Tested
Not recommended
Tested
Expected
Expected
Rat
Tested
Not recommended
Tested
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation. HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal RKIP antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 20 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2875Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Raf kinase inhibitory protein (RKIP) also known as phosphatidylethanolamine-binding protein 1 (PEBP1) is a significant regulatory protein weighing around 21 kDa. It inhibits the RAF/MEK/ERK signaling cascade by binding to Raf-1 kinase. RKIP shows expression in diverse tissues including the brain heart and liver reflecting its widespread influence across various physiological systems.

Biological function summary

RKIP interacts with multiple signaling pathways by acting as a suppressor of the mitogen-activated protein kinase (MAPK) pathway. It also plays roles in modulating the signaling of nuclear factor kappa B (NF-κB) and G-protein coupled receptor kinase (GRK) pathways. As part of these pathways RKIP contributes to the regulation of cell proliferation differentiation and apoptosis making it an important player in maintaining cellular homeostasis.

Pathways

RKIP's role centers on the RAF/MEK/ERK and NF-κB signaling pathways. Within the RAF/MEK/ERK pathway RKIP modulates cellular responses by controlling the activation of Raf-1 influencing cell growth and division. In the NF-κB pathway RKIP inhibits phosphorylation of IKK thereby impacting inflammatory responses which links RKIP to proteins like MEK and ERK as well as IKK.

Associated diseases and disorders

RKIP has involvement in cancer and cardiovascular diseases. Reduced RKIP expression associates with increased metastasis in cancers such as prostate and breast cancer indicating its role as a metastasis suppressor. In cardiovascular contexts RKIP affects hypertension pathogenesis through the modulation of GRK pathway involving connections to proteins such as Raf-1 and IKK.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (ab76582), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling RKIP with purified ab76582 at 1:50 dilution (2.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582), expandable thumbnail

    Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

    All lanes: Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: Mouse brain lysates at 20 µg

    Lane 4: Mouse testis lysates at 20 µg

    Lane 5: Rat brain lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582), expandable thumbnail

    Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

    All lanes: Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: A549 cell lysate at 10 µg

    Lane 3: HepG2 cell lysate at 10 µg

    Lane 4: A431 cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 21 kDa

    Observed band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582 (unpurified).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582), expandable thumbnail

    Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

    All lanes: Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution

    All lanes: Western blot - Recombinant Human RKIP protein (Recombinant Human RKIP protein ab87892) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 21 kDa

    Exposure time: 30s

  • Flow Cytometry (Intracellular) - Anti-RKIP antibody [EPR2875Y] (ab76582), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-RKIP antibody [EPR2875Y] (ab76582)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RKIP (red) with purified ab76582 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

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