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AB241959

Anti-RME-8 antibody

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(1 Publication)

Rabbit Polyclonal RME-8 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DNAJC13 aa 1150-1250.

View Alternative Names

KIAA0678, RME8, DNAJC13, DnaJ homolog subfamily C member 13, Required for receptor-mediated endocytosis 8, RME-8

2 Images
Western blot - Anti-RME-8 antibody (AB241959)
  • WB

Supplier Data

Western blot - Anti-RME-8 antibody (AB241959)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-RME-8 antibody (ab241959) at 0.4 µg

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 254 kDa

true

Exposure time: 3min

Immunoprecipitation - Anti-RME-8 antibody (AB241959)
  • IP

Supplier Data

Immunoprecipitation - Anti-RME-8 antibody (AB241959)

RME-8 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241959 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab241959 at 1 μg/ml.

Lane 1 : ab241959 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-RME-8 antibody (ab241959)

Predicted band size: 254 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DNAJC13 aa 1150-1250. The exact immunogen used to generate this antibody is proprietary information.

O75165

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was affinity purified using an epitope specific to RME-8 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RME-8 also known as DNAJC13 is a protein that functions as an ATPase assisting in the regulation of clathrin-mediated endocytosis. It possesses a mass of approximately 218 kDa and is mainly found in the central nervous system though it is also present in various other tissues. Mechanically RME-8 binds to clathrin-coated vesicles and plays a part in rearranging them during endocytosis. It interacts with multiple factors involved in membrane trafficking contributing to the modulation of vesicle fission and fusion events.
Biological function summary

The activity of RME-8 is essential for the maintenance of cellular homeostasis. It interacts with other proteins to form a complex that facilitates proper protein sorting within the endosomal network. RME-8's role in this intricate system ensures the appropriate recycling of receptors and disposal of damaged cellular components aiding in overall cell function. The protein regulates the spatial organization of endosomes an important process necessary for nutrient uptake and receptor recycling which impacts cell signaling and metabolism.

Pathways

RME-8 participates in endocytic pathways where it collaborates with proteins like Eps15 and Hsc70 to manage cargo trafficking. The endocytic pathway heavily relies on RME-8's functions for sustaining efficient internalization and routing of molecules such as receptors and ligands. Additionally the protein interacts with components of the retromer complex indicating a connection to the retromer-mediated transport pathway. This involvement may have effects on intracellular transport activities influencing the broader cellular communication network.

RME-8 has connections to neurodegenerative disorders such as Parkinson's disease. Mutations in the DNAJC13 gene which codes for RME-8 have been linked to familial parkinsonism. The dysfunction of RME-8 affects endosomal processes potentially contributing to the pathophysiology of Parkinson's by impacting dopamine neuron survival. It is also implicated in Alzheimer's disease with disruptions in its role possibly affecting amyloid-beta processing through its interactions with proteins like Sortilin-related receptor 1 (SORL1) which is known to influence amyloid precursor protein sorting and degradation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in membrane trafficking through early endosomes, such as the early endosome to recycling endosome transport implicated in the recycling of transferrin and the early endosome to late endosome transport implicated in degradation of EGF and EGFR (PubMed : 18256511, PubMed : 18307993). Involved in the regulation of endosomal membrane tubulation and regulates the dynamics of SNX1 on the endosomal membrane; via association with WASHC2 may link the WASH complex to the retromer SNX-BAR subcomplex (PubMed : 24643499).
See full target information DNAJC13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 131: PubMed33079728

2020

Guanosine triphosphate links MYC-dependent metabolic and ribosome programs in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Huang,Kenneth E Huffman,Zixi Wang,Xun Wang,Kailong Li,Feng Cai,Chendong Yang,Ling Cai,Terry S Shih,Lauren G Zacharias,Andrew Chung,Qian Yang,Milind D Chalishazar,Abbie S Ireland,C Allison Stewart,Kasey Cargill,Luc Girard,Yi Liu,Min Ni,Jian Xu,Xudong Wu,Hao Zhu,Benjamin Drapkin,Lauren A Byers,Trudy G Oliver,Adi F Gazdar,John D Minna,Ralph J DeBerardinis
View all publications
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