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AB122685

Anti-RMI2 antibody

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(6 Publications)

Rabbit Polyclonal RMI2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RMI2 aa 50 to C-terminus.

View Alternative Names

C16orf75, RMI2, RecQ-mediated genome instability protein 2, hRMI2, BLM-associated protein of 18 kDa, BLAP18

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RMI2 antibody (AB122685)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RMI2 antibody (AB122685)

ab122685, at 1/200 dilution, staining RMI2 in paraffin-embedded Human stomach tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-RMI2 antibody (AB122685)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RMI2 antibody (AB122685)

ab122685, at 4 μg/ml staining RIM2 in PFA/Triton X-100 fixed/permeabilized U 251 MG cells by Immunofluorescence (green).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RMI2 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96E14

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RMI2 also known as RecQ Mediated Genome Instability 2 or BLAP75 is a protein involved in maintaining genome stability. This protein is important in the DNA repair processes and has a molecular weight of about 60 kDa. RMI2 is mainly expressed in tissues with high proliferative rates including testis and bone marrow. Its expression is not limited to these tissues indicating a broader role in cellular activity.
Biological function summary

This protein works in coordination with other components for efficient DNA repair. RMI2 is a part of the BTR complex which includes BLM helicase topoisomerase IIIα and RMI1. Within this complex RMI2 acts to stabilize the structure and enhance the function of the helicase and topoisomerase activities. Its role ensures proper resolution of DNA structures during replication and repair preventing genomic instability and preserving cell integrity.

Pathways

RMI2 contributes to the homologous recombination and DNA damage response pathways. In these pathways it interacts with proteins such as BLM and RMI1. Together they play an important role in the accurate separation of homologous chromosomes and repair of double-strand breaks which are essential for genome maintenance and cell survival. RMI2 enhances the BTR complex's ability to manage replication stress and prevent inappropriate recombination events.

RMI2 has been linked to certain cancer types including leukemia and colon cancer. In these diseases dysregulated expression or mutations of RMI2 can influence the stability of the genome contributing to tumorous growth. Additionally the interactions between RMI2 and BLM are significant as mutations in BLM can lead to Bloom syndrome characterized by genomic instability and cancer predisposition. Understanding these interactions is important for potential therapeutic targets in cancer treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates. It is required to regulate sister chromatid segregation and to limit DNA crossover. Essential for the stability, localization, and function of BLM, TOP3A, and complexes containing BLM. In the RMI complex, it is required to target BLM to chromatin and stress-induced nuclear foci and mitotic phosphorylation of BLM.
See full target information RMI2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8476 PubMed41006228

2025

EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Marija Maric,Sandra Segura-Bayona,Raviprasad Kuthethur,Tohru Takaki,Valerie Borel,Tyler H Stanage,Miroslav P Ivanov,Nishita Parnandi,Graeme Hewitt,Rhona Millar,Carmen S Fonseca,Harshil Patel,Miriam Llorian,Scott Warchal,Michael Howell,Arnab Ray Chaudhuri,Panagiotis Kotsantis,Simon J Boulton

Cancer medicine 12:8331-8350 PubMed36533385

2022

RMI2 is a novel prognostic and predictive biomarker for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lijie Zhang,Chuncheng Hao,Baojuan Han,Guangchun Zeng,Lili Han,Cong Cao,Hui Liu,Zhenbin Zhong,Xue Zhao,Jingxuan Wang,Qingyuan Zhang

Technology in cancer research & treatment 20:15330338211045496 PubMed34634948

2021

The Clinical Significance of RMI2 in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Zheng Ms,Heng Wang Ms,Jin-Xue Wang Ms,Zheng-Hong Liu Ms,Pu Zhang Md,Dahong Zhang Md

Biochimica et biophysica acta. Molecular basis of disease 1867:166106 PubMed33631320

2021

Predominant cellular mitochondrial dysfunction in the TOP3A gene-caused Bloom syndrome-like disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Jiang,Nan Jia,Chaowan Guo,Juan Wen,Lingqian Wu,Tomoo Ogi,Huiwen Zhang

Molecular cell 69:9-23.e6 PubMed29290614

2018

Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Nicholls,Cristina A Nadalutti,Elisa Motori,Ewen W Sommerville,Gráinne S Gorman,Swaraj Basu,Emily Hoberg,Doug M Turnbull,Patrick F Chinnery,Nils-Göran Larsson,Erik Larsson,Maria Falkenberg,Robert W Taylor,Jack D Griffith,Claes M Gustafsson

Clinical cancer research : an official journal of the American Association for Cancer Research 24:696-707 PubMed29138344

2017

Targeting the MYCN-PARP-DNA Damage Response Pathway in Neuroendocrine Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Bo Liu,Wenhui Wu,Likun Li,Bradley M Broom,Spyridon P Basourakos,Dimitrios Korentzelos,Yang Luan,Jianxiang Wang,Guang Yang,Sanghee Park,Abul Kalam Azad,Xuhong Cao,Jeri Kim,Paul G Corn,Christopher J Logothetis,Ana M Aparicio,Arul M Chinnaiyan,Nora Navone,Patricia Troncoso,Timothy C Thompson
View all publications
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