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AB254902

Anti-RMND5A antibody

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(2 Publications)

Rabbit Polyclonal RMND5A antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RMND5A aa 50 to C-terminus.

View Alternative Names

E3 ubiquitin-protein transferase RMND5A, P44CTLH, Protein RMD5 homolog A, RMND5A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RMND5A antibody (AB254902)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RMND5A antibody (AB254902)

Paraffin-embedded human kidney tissue stained for RMND5A using ab254902 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-RMND5A antibody (AB254902)
  • WB

Supplier Data

Western blot - Anti-RMND5A antibody (AB254902)

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Western blot - Anti-RMND5A antibody (ab254902)

Lanes 2 - 3:

Western blot - Anti-RMND5A antibody (ab254902) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

RT4 cell lysate

Lane 3:

U-251 MG cell lysate

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RMND5A aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H871

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RMND5A also known as Required for Meiotic Nuclear Division 5 Homolog A functions as an E3 ubiquitin-protein ligase with a mass of approximately 59 kDa. This protein is centrally involved in tagging specific substrates with ubiquitin for their subsequent degradation by the proteasome a process essential for maintaining cellular homeostasis. RMND5A is widely expressed across various tissues and cell types suggesting its important cellular role.
Biological function summary

The RMND5A protein takes part in diverse cellular processes often acting as a component of the CTLH complex also known as the C-terminal to LisH complex. This complex is involved in regulating cell cycle progression and signaling pathways by specifically targeting proteins for ubiquitination and degradation. Through its activity RMND5A influences cell proliferation and differentiation by modulating the levels of key regulatory proteins within the cell.

Pathways

RMND5A plays a role in the ubiquitin-proteasome system and the Wnt signaling pathway. In these pathways RMND5A interacts with other proteins such as GID8 (also a component of the CTLH complex) and APC in Wnt signaling. In the context of the ubiquitin-proteasome system RMND5A assists in maintaining protein quality control by ensuring the removal of defective or unneeded proteins a function critical for cellular regulation.

RMND5A has connections with certain cancers and neurodegenerative diseases. Alterations in RMND5A expression or function can disrupt cellular protein homeostasis leading to oncogenic transformation or neuronal cell death. The protein interacts with p53 in cancer pathways and with tau proteins in neurodegenerative contexts emphasizing its relevance in disease mechanisms. Understanding RMND5A's function further can aid in developing targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. MAEA and RMND5A are both required for catalytic activity of the CTLH E3 ubiquitin-protein ligase complex (PubMed : 29911972). Catalytic activity of the complex is required for normal cell proliferation (PubMed : 29911972). The CTLH E3 ubiquitin-protein ligase complex is not required for the degradation of enzymes involved in gluconeogenesis, such as FBP1 (PubMed : 29911972).
See full target information RMND5A
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 22:532 PubMed34079591

2021

miR-590-5p targets RMND5A and promotes migration in pancreatic adenocarcinoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Sixing Chen,Yu Chen,Yao Wen,Wanwan Cai,Ping Zhu,Wuzhou Yuan,Yongqing Li,Xiongwei Fan,Yongqi Wan,Fang Li,Jian Zhuang,Zhigang Jiang,Xiushan Wu,Yuequn Wang

Journal of experimental & clinical cancer research : CR 40:134 PubMed33858489

2021

The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulates the proliferation of glioma stem cells via the IL6-mediated JAK2/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junshuang Zhao,Yang Jiang,Haiying Zhang,Jinpeng Zhou,Lian Chen,Hao Li,Jinkun Xu,Guoqing Zhang,Zhitao Jing
View all publications
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Product promise

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