AB202438

Anti-RMS1 antibody [EPR20103]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal RMS1 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 1 publication.

View Alternative Names

RMS1, SET7, YDR257C, YD9320A.07C, RKM4, Ribosomal lysine N-methyltransferase 4, SET domain-containing protein 7

2 Images

Supplier Data

Western blot - Anti-RMS1 antibody [EPR20103] (AB202438)

Blocking and dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-RMS1 antibody [EPR20103] (ab202438) at 1/1000 dilution

All lanes:

Saccharomyces cerevisiae whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-RMS1 antibody [EPR20103] (AB202438)

Blocking and dilution buffer : 5% NFDM/TBST

RKM4 KO strain (Y03616) was purchased from Euroscarf.

All lanes:

Western blot - Anti-RMS1 antibody [EPR20103] (ab202438) at 1/1000 dilution

Lane 1:

Saccharomyces cerevisiae (wild-type) whole cell lysate at 10 µg

Lane 2:

Saccharomyces cerevisiae (RKM4 KO) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

true

Exposure time: 3min

Carrier free

Anti-RMS1 antibody [EPR20103] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20103

Isotype

IgG

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RMS1 also known as Rhomboid-like protein is a transmembrane serine protease with a molecular mass of approximately 55 kDa. It locates primarily on the inner mitochondrial membrane and is widely expressed across various tissues including the heart brain and liver. RMS1 belongs to the family of rhomboid proteases which perform intramembrane proteolysis. This protease modulates protein processing by cleaving specific substrate proteins within the membrane environment influencing their function and stability.

Biological function summary

This protein contributes to cellular homeostasis by regulating protein quality control and mitochondrial function. It engages in the organized removal of misfolded or damaged proteins within the mitochondria working as part of multiprotein complexes. RMS1 plays a significant role in the control of a subset of substrates ensuring proteins are maintained in their functional forms or cleared if defective which is critical for cellular metabolism and overall energy production.

Pathways

RMS1 interacts with proteolytic and mitochondrial homeostasis pathways. It participates in a pathway linked with mitochondrial proteostasis closely interacting with other proteins like PARL and PINK1 to ensure mitochondrial quality control. RMS1's proteolytic activity adjusts the protein components within the mitochondria aligning with the cell's metabolic demands and signaling needs which is vital for cell survival and adaptation to stress.

Defects or dysregulation of RMS1 have been linked to neurodegenerative conditions such as Parkinson's disease. RMS1 affects and connects with other key proteins involved in these disorders including DJ-1 and PINK1 which are vital for maintaining mitochondrial health. Aberrant function of RMS1 disrupts the meticulously balanced environment of protein turnover in mitochondria leading to the accumulation of harmful protein aggregates and oxidative stress which contributes to neuronal cell death.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

S-adenosyl-L-methionine-dependent protein-lysine N-methyltransferase that monomethylates 60S ribosomal protein L42 (RPL42A and RPL42B) at 'Lys-55'.

See full target information RKM4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Prostate 80:1145-1156 PubMed32659025

2020

CXCL12γ induces human prostate and mammary gland development.

Applications

Unspecified application

Species

Unspecified reactive species

Younghun Jung,Jin Koo Kim,Eunsohl Lee,Frank C Cackowski,Ann M Decker,Paul H Krebsbach,Russell S Taichman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RMS1 antibody [EPR20103]

Western blot - Anti-RMS1 antibody [EPR20103] (AB202438)

Western blot - Anti-RMS1 antibody [EPR20103] (AB202438)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise