Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RNA Helicase A with purified ab183731 at 1/20 dilution (5μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

Immunocytochemistry analysis of HeLa cells (fixative -20? Acetone) labeling RNA Helicase A with ab183731 (unpurified) at a 1/250 dilution.Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RNA Helicase A with purified ab183731 at 1/50 dilution (2.2 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling RNA Helicase A with purified ab183731 at 1/400 dilution (0.28 μg/mL). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labeling RNA Helicase A with ab183731 (unpurified) at a 1/100 dilution. Prediluted HRP conjugated Rabbit IgG secondary used. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

Intracellular Flow Cytometry analysis of HeLa cells using ab183731 (unpurified) at a 1/10 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731). Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RNA Helicase A with ab183731 at 1/1000 (0.11 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). This section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Nuclear staining on human testis. The section was incubated with ab183731 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731). Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling RNA Helicase A with ab183731 at 1/1000 (0.11 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). This section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Nuclear staining on human breast carcinoma. The section was incubated with ab183731 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731). Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling RNA Helicase A with ab183731 at 1/1000 (0.11 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). This section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Nuclear staining on rat testis. The section was incubated with ab183731 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731). Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling RNA Helicase A with ab183731 at 1/1000 (0.11 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). This section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Nuclear staining on mouse testis. The section was incubated with ab183731 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731). Immunohistochemical analysis of paraffin-embedded Mouse breast carcinoma tissue labeling RNA Helicase A with ab183731 at 1/1000 (0.11 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). This section was counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Nuclear staining on mouse breast carcinoma. The section was incubated with ab183731 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• WB

Lab

Western blot - Anti-RNA Helicase A antibody [EPR13521] - BSA and Azide free (AB238985)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183731). Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control. Exposure time : Lanes 1-5 : 103 seconds, Lane 6 : 26 seconds. Lysates were freshly made and used immediately to minimize protein degradation.

All lanes:

Western blot - Anti-RNA Helicase A antibody [EPR13521] (<a href='/en-us/products/primary-antibodies/rna-helicase-a-antibody-epr13521-ab183731'>ab183731</a>) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Rat testis tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 141 kDa

Observed band size: 150 kDa

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