Anti-RNA polymerase beta antibody [EPR18704]

• IP

Supplier Data

Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (AB191598)

RNA polymerase beta was immunoprecipitated from 1mg of E.coli whole cell lysates with ab191598 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191598 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : E.coli whole cell lysates 10ug (Input). Lane 2 : ab191598 IP in E.coli whole cell lysates. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191598 in E.coli whole cell lysates.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)

false

• WB

Supplier Data

Western blot - Anti-RNA polymerase beta antibody [EPR18704] (AB191598)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RNA polymerase beta antibody [EPR18704] (ab191598) at 1/2000 dilution

All lanes:

E.coli whole cell lysates at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 150 kDa

Observed band size: 150 kDa

false

Exposure time: 30s