AB240324

Anti-RNA polymerase beta antibody [EPR18704] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RNA polymerase beta antibody. Carrier free. Suitable for IP, WB and reacts with Escherichia coli samples. Cited in 1 publication.

View Alternative Names

groN, nitB, rif, ron, stl, stv, tabD, b3987, JW3950, rpoB, DNA-directed RNA polymerase subunit beta, RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta

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Supplier Data

Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] - BSA and Azide free (AB240324)

RNA polymerase beta was immunoprecipitated from 1mg of E.coli whole cell lysates with ab191598 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191598 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Lane 1 : E.coli whole cell lysates 10ug (Input). Lane 2 : ab191598 IP in E.coli whole cell lysates. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191598 in E.coli whole cell lysates.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab191598).

All lanes:

Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (<a href='/en-us/products/primary-antibodies/rna-polymerase-beta-antibody-epr18704-ab191598'>ab191598</a>)

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18704

Isotype

IgG

Carrier free

Yes

Reacts with

Escherichia coli

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab240324 is the carrier-free version of ab191598.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNA polymerase beta also known as RpoB or DNA-directed RNA polymerase subunit beta is a critical enzyme in the transcription process. This protein has a molecular mass of approximately 150 kDa. RpoB plays a role by catalyzing the synthesis of RNA from a DNA template an essential step in gene expression. It is widely expressed in bacteria mitochondria chloroplasts and also in the nuclei of eukaryotic cells. RpoB functions as a fundamental part of the core RNA polymerase complex which also includes other subunits such as alpha beta' and omega.

Biological function summary

RpoB interacts with multiple subunits to form the RNA polymerase holoenzyme integrating into cellular machinery to initiate transcription. This enzyme participates in synthesizing mRNA rRNA and tRNA all of which are important for protein synthesis. The holoenzyme formation aligns with promoter regions on DNA enabling precise transcription initiation. Without the proper function of RpoB and its associated subunits transcription efficiency and fidelity could be compromised impacting cell viability.

Pathways

RNA polymerase beta is integral to transcriptional regulation pathways and genetic response mechanisms within the cell. It plays a role in the bacterial transcription pathway directly interacting with sigma factors and other transcription regulators to manage gene expression in response to environmental changes. Furthermore RpoB operates in conjunction with other proteins like sigma factors which allow the enzyme to recognize specific promoter sequences ensuring the transcription of appropriate genes.

The improper function of RNA polymerase beta has correlations with bacterial resistance to antibiotics notably rifampicin. Mutations in the rpoB gene can lead to resistance complicating treatment strategies for conditions like tuberculosis. This enzyme's relationship with sigma factors also presents a route through which these mutations affect transcription fidelity leading to resistant bacterial strains. Understanding these connections helps to develop therapies targeting the bacterial transcription machinery effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.. Resistance to the antibiotics salinamide A, salinamide B, rifampicin, streptolydigin, CBR703, myxopyronin, and lipiarmycin can result from mutations in this protein.. Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNAP. It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis.

See full target information rpoB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:105723 PubMed36590169

2023

Assessment of stromal SCD-induced drug resistance of PDAC using 3D-printed zPDX model chips.

Applications

Unspecified application

Species

Unspecified reactive species

Chuntao Wu,Beiyuan Hu,Lei Wang,Xia Wu,Haitao Gu,Hanguang Dong,Jiuliang Yan,Zihao Qi,Qi Zhang,Huan Chen,Bo Yu,Sheng Hu,Yu Qian,Shuang Dong,Qiang Li,Xu Wang,Jiang Long

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RNA polymerase beta antibody [EPR18704] - BSA and Azide free

Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] - BSA and Azide free (AB240324)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise