Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10]
- Recombinant
- What is this?
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(9 Publications)
Rat Recombinant Monoclonal RNA polymerase II RPB1 phospho S2 antibody. Suitable for ChIP, Dot, WB, I-ELISA, ICC/IF and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples. Cited in 9 publications.
View Alternative Names
POLR2, POLR2A, DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, 3'-5' exoribonuclease, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (AB252855)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling RNA polymerase II CTD repeat YSPTSPS (phospho S2) with ab252855 at 1/500 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line, the signal decreased after phosphatase treatment at 37°C for 2h. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
- ChIP
Unknown
ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (AB252855)
Chromatin was prepared from HeLa cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 5 μg of ab252855 (red), or 5 μg of Rat IgG1 ab18407 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (AB252855)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 cells labelling RNA polymerase II CTD repeat YSPTSPS (phospho S2) with ab252855 at 1/500 dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in RAW 264.7 cell line, the signal decreased after phosphatase treatment at 37°C for 2h. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 4 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control : Secondary antibody is ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
- WB
Lab
Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (AB252855)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 22745433 and 23071310)
Exposure time : 3 seconds
All lanes:
Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (ab252855) at 1/1000 dilution
Lane 1:
HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate (Untreated membrane) at 10 µg
Lane 2:
HeLa whole cell lysate (Phosphatase treated membrane) at 10 µg
Secondary
All lanes:
Goat Anti-Rat IgG (H+L), HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution
Predicted band size: 217 kDa
Observed band size: 260 kDa
false
- WB
Lab
Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (AB252855)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 22745433 and 23071310)
Exposure time : 7 seconds
All lanes:
Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (ab252855) at 1/1000 dilution
Lane 1:
RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate (Untreated membrane) at 10 µg
Lane 2:
RAW264.7 whole cell lysate (Phosphatase treated membrane) at 10 µg
Secondary
All lanes:
Goat Anti-Rat IgG (H+L), HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution
Predicted band size: 217 kDa
Observed band size: 260 kDa
false
- WB
Lab
Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (AB252855)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 22745433 and 23071310)
Exposure time : 3 seconds
All lanes:
Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (ab252855) at 1/1000 dilution
Lane 1:
PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 2:
PC-12 whole cell lysate (Phosphatase treated membrane) at 10 µg
Secondary
All lanes:
Goat Anti-Rat IgG (H+L), HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/5000 dilution
Predicted band size: 217 kDa
Observed band size: 260 kDa
false
- I-ELISA
Unknown
Indirect ELISA - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (AB252855)
ELISA analysis using ab252855 at a range of 1000-0 ng/ml followed by a Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) at 1/1000 dilution. Antigen concentration : 1000 ng/ml
Antigens : phospho S2, phospho S5, phospho T4, phospho Y1, phospho S7, non-phospho
- Dot
Supplier Data
Dot Blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] (AB252855)
Dot blot analysis using ab252855 at 1/1000 dilution followed by a Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/5000 dilution. Blocking/diluting buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes.
Lane 1 : RNA polymerase II CTD repeat YSPTSPS (phospho S2) peptide
Lane 2 : RNA polymerase II CTD repeat YSPTSPS (phospho S5) peptide
Lane 3 : RNA polymerase II CTD repeat YSPTSPS (phospho T4) peptide
Lane 4 : RNA polymerase II CTD repeat YSPTSPS (phospho Y1) peptide
Lane 5 : RNA polymerase II CTD repeat YSPTSPS (phospho S7) peptide
Lane 6 : RNA polymerase II CTD repeat YSPTSPS non-phopho peptide
Related conjugates and formulations (1)
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Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S2) antibody [3E10] - BSA and Azide free
Reactivity data
Product details
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Properties and storage information
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Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
RNA polymerase II CTD repeat YSPTSPS is essential for the transcription progression from initiation to termination. It is part of the large RNA polymerase II complex interacting with various transcription factors and enzymes necessary for RNA processing. The phosphorylation state of the CTD particularly on serine residues regulates interactions with splicing machinery and other RNA processing factors. This modulation ensures the coupling between transcription and RNA processing events controlling mRNA synthesis and maturation.
Pathways
RNA polymerase II CTD repeat YSPTSPS is important in the mRNA synthesis pathway specifically in transcriptional regulation and processing of nascent RNA transcripts. It interacts with proteins such as the transcription factors TFIIH and TFIIB which aid in promoter recognition and open complex formation. The CTD's dynamic phosphorylation pattern allows integration into multiple cellular pathways most importantly connecting transcription with RNA splicing and transport pathways.
Product protocols
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Target data
Publications (9)
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Journal of experimental & clinical cancer research : CR 44:95 PubMed40069781
2025
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Nature 635:210-218 PubMed39506153
2024
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Nature 635:201-209 PubMed39506152
2024
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Nature communications 15:6829 PubMed39122693
2024
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Genome biology 25:54 PubMed38388963
2024
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PLoS biology 21:e3002161 PubMed37379335
2023
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Nutrients 15: PubMed37375678
2023
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International journal of molecular sciences 23: PubMed36012127
2022
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The Journal of cell biology 220: PubMed33443568
2021
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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