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Rabbit Polyclonal RNA polymerase II RPB1 phospho S2 antibody. Suitable for ICC/IF, WB, ELISA, IHC-P and reacts with Human, Mouse, Saccharomyces cerevisiae, Rat, Synthetic peptide - Human, Synthetic peptide - Saccharomyces cerevisiae samples. Cited in 541 publications.
Alternative names=DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit 1, RNA polymerase II subunit B1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B220, RPB220, RPB1, RPO21, SUA8, YDL140C, D2150
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| ICC/IF | WB | ELISA | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested |
| Mouse | Tested | Expected | Expected | Tested |
| Rat | Expected | Expected | Predicted | Tested |
| Arabidopsis thaliana | Predicted | Predicted | Predicted | Predicted |
| Caenorhabditis elegans | Predicted | Predicted | Predicted | Predicted |
| Drosophila melanogaster | Predicted | Predicted | Predicted | Predicted |
| Saccharomyces cerevisiae | Expected | Tested | Expected | Expected |
| Schizosaccharomyces pombe | Predicted | Predicted | Predicted | Predicted |
| Synthetic peptide - Human | Not recommended | Not recommended | Expected | Not recommended |
| Synthetic peptide - Saccharomyces cerevisiae | Not recommended | Not recommended | Expected | Not recommended |
| Xenopus laevis | Predicted | Predicted | Predicted | Predicted |
Images
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Synthetic peptide - Saccharomyces cerevisiae | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Synthetic peptide - Saccharomyces cerevisiae | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Synthetic peptide - Saccharomyces cerevisiae, Mouse, Human, Saccharomyces cerevisiae | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Xenopus laevis, Arabidopsis thaliana, Caenorhabditis elegans, Drosophila melanogaster, Schizosaccharomyces pombe | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Synthetic peptide - Saccharomyces cerevisiae | Dilution info - | Notes - |
Recommended products
Rabbit Polyclonal RNA polymerase II RPB1 phospho S2 antibody. Suitable for ICC/IF, WB, ELISA, IHC-P and reacts with Human, Mouse, Saccharomyces cerevisiae, Rat, Synthetic peptide - Human, Synthetic peptide - Saccharomyces cerevisiae samples. Cited in 541 publications.
Alternative names=DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit 1, RNA polymerase II subunit B1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B220, RPB220, RPB1, RPO21, SUA8, YDL140C, D2150
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. This antibody recognises the phosphorylated serine found in the amino acid 2 position of the C-terminal domain repeat YSPTSPS.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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