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Mouse Monoclonal RNA polymerase II RPB1 phospho S5 antibody. Suitable for ChIP, Flow Cyt (Intra), ELISA, Dot, WB, ICC/IF and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples. Cited in 362 publications.
Alternative names=DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, POLR2, POLR2A
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ChIP | Flow Cyt (Intra) | ELISA | Dot | WB | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected | Tested | Tested |
| Mouse | Expected | Expected | Expected | Expected | Tested | Expected |
| Rat | Expected | Expected | Expected | Expected | Tested | Expected |
| Arabidopsis thaliana | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
| Synthetic peptide - Human | Not recommended | Not recommended | Tested | Tested | Not recommended | Not recommended |
Images
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-4 µg for 106 Cells | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Arabidopsis thaliana | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Arabidopsis thaliana | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Arabidopsis thaliana | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Arabidopsis thaliana | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Arabidopsis thaliana | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Arabidopsis thaliana | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Recommended products
Mouse Monoclonal RNA polymerase II RPB1 phospho S5 antibody. Suitable for ChIP, Flow Cyt (Intra), ELISA, Dot, WB, ICC/IF and reacts with Human, Mouse, Rat, Synthetic peptide - Human samples. Cited in 362 publications.
Alternative names=DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1, POLR2, POLR2A
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
The exact immunogen used to generate this antibody is proprietary information.
- Clone number
4H8
- Purification technique
Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
ChIP protocols:
ChIP protocol for cross-linking ChIP (X-ChIP)
Native ChIP protocol
Chromatin preparation from tissues for ChIP
ChIP troubleshooting
ChIP tips and tricks guide
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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- Visit the general protocols
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