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AB5131

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody

4

(48 Reviews)

|

(497 Publications)

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) is a rabbit polyclonal antibody for Western Blot, IP, IHC, ICC/IF, ELISA, ChIP in Arabidopsis, C.elegans, Drosophila, Human, Mouse, Rat, S.cerevisiae, S.pombe, Xenopus, Zebrafish.

- Over 440 publications
- Trusted since 2005

View Alternative Names

POLR2, POLR2A, DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, 3'-5' exoribonuclease, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1

7 Images
ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)
  • ChIP

Unknown

ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

Chromatin was prepared from U-2 OS (Human bone osteosarcoma epithelial cell line) cells according to the Abcam X-ChIP protocol.

Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25 μg of chromatin, 2 μg of ab5131 (blue), and 20 μl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified on the inactive AFM and F8 promoters, the GAPDH promoter (active) and over the y-Actin gene (active).

Schematic diagram of the y-Actin gene is shown on the top of the figure. Black boxes represent exons and thin lines represent introns. PCR products are depicted as bars under the gene.

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

ab5131 staining RNA polymerase II CTD repeat YSPTSPS (phospho S5) in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab5131 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

ICC/IF image of ab5131 stained HeLa(Human epithelial cell line from cervix adenocarcinoma) cells.

The cells were fixed in methanol for 5 minutes, permabilized in TBS-T for 20 minutes and incubated with the antibody (ab5131, 1 μg/ml) for 1 hour at room temperature. 1% BSA / 10% normal goat serum / 0.3M glycine was used to quench auto-fluorescence and block non-specific protein-protein interactions.

The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)
  • ChIP

PubMed

ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

Chromatin from cultured cells, mouse PVN punches (individual pools formed from groups of 3) or whole hypothalami dissected from fresh brains were cross linked, disrupted by sonification and purified.

The binding of Suz12 to wingless (Wnt1), a beta-catenin dependent developmental regulator and to the RNA polymerase II promoter (RNAPII), a housekeeping gene, served as positive and negative controls, respectively, in these experiments.

Murgatroyd et al PLoS One. 2014 Mar 5;9(3):e90277. doi: 10.1371/journal.pone.0090277. eCollection 2014. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)
  • WB

Project5649****

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

All lanes:

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1 µg/mL

Lanes 1, 5 and 7:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg

Lanes 2, 6 and 8:

S.cerevisiae (Y190) Whole Cell Lysate at 20 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 20 µg with <em>S. cerevisiae</em> RNA polymerase II CTD repeat YSPTSPS peptide (<a href='/en-us/products/unavailable/s-cerevisiae-rna-polymerase-ii-ctd-repeat-ysptsps-peptide-ab12795'>ab12795</a>)

Lane 4:

S.cerevisiae (Y190) Whole Cell Lysate at 20 µg with <em>S. cerevisiae</em> RNA polymerase II CTD repeat YSPTSPS peptide (<a href='/en-us/products/unavailable/s-cerevisiae-rna-polymerase-ii-ctd-repeat-ysptsps-peptide-ab12795'>ab12795</a>)

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 217 kDa

true

Exposure time: 30s

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)
  • WB

AbReview40285****

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

All lanes:

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1/5000 dilution

Lane 1:

Xenopus laevis whole tissue lysate treated with DMSO for 24 hours

Lane 2:

Xenopus laevis whole tissue lysate treated with CDK inhibitor for 24 hours

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

Predicted band size: 217 kDa

Observed band size: 240 kDa

true

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)
  • WB

Unknown

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

Lanes 1, 3 and 4:

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1/500 dilution

Lane 2:

Western blot - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) at 1/2000 dilution

Lanes 1 - 3:

HeLa nuclear extract at 20 µg

Lane 4:

HeLa nuclear extract at 20 µg with <em>S. cerevisiae</em> RNA polymerase II CTD repeat YSPTSPS peptide (<a href='/en-us/products/unavailable/s-cerevisiae-rna-polymerase-ii-ctd-repeat-ysptsps-peptide-ab12795'>ab12795</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab6721'>ab6721</a>) at 1/5000 dilution

Predicted band size: 217 kDa

Observed band size: 250 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Zebrafish, Mouse, Saccharomyces cerevisiae, Arabidopsis thaliana, Schizosaccharomyces pombe, Xenopus laevis, Rat, Caenorhabditis elegans, Drosophila melanogaster

Applications

IHC-Fr, WB, ICC/IF, IHC - Wmt, ELISA, ChIP, IP, IHC-P

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

The exact immunogen used to generate this antibody is proprietary information.

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody recognises the phosphorylated serine found in the amino acid 5 position of the C-terminal domain repeat YSPTSPS of RNA polymerase II. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

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"IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Schizosaccharomyces pombe": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", 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"IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Zebrafish": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "2 µg for 25 µg chromatin", "ChIP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCWmt-species-checked": "guaranteed", "IHCWmt-species-dilution-info": "", "IHCWmt-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) is a rabbit polyclonal antibody and is validated for use in ChIP, ELISA, ICC/IF, IHC - Wmt, IHC-Fr, IHC-P, IP, WB.

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) has been cited over 444 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) has high sensitivity and specificity.

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) has 48 independent reviews from customers.

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (ab5131) specifically detects RNA polymerase II CTD repeat YSPTSPS Phospho-S5 (UniProt ID: P24928; Molecular weight: 217kDa) and is sold in 50 µg selling sizes.

RNA polymerase II CTD repeat YSPTSPS (phospho S5) is crucial for transcription regulation and gene expression. Research indicates that phosphorylation at serine 5 is essential for the transition from transcription initiation to elongation, impacting various cellular processes and disease states. Phosphorylation of RNA polymerase II at serine 5 is critical for proper mRNA synthesis and processing. Abnormal phosphorylation patterns are linked to transcriptional dysregulation in cancer, pediatric gliomas, chondroblastomas, and neurodevelopmental syndromes. Studying these modifications can provide insights into potential therapeutic targets for various conditions.

Phosphorylation of RNA polymerase II's largest subunit C-terminal domain (CTD) is a key event during mRNA metabolism.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 98.98% PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNA polymerase II CTD repeat YSPTSPS also known as the C-terminal domain of RNA polymerase II is a critical component of the RNA polymerase II enzyme commonly referred to as pol II. This domain is characterized by the repetitive sequence YSPTSPS which plays a significant role in the regulation of transcription. The mass of RNA polymerase II including its CTD varies but is essential for its function in gene expression. RNA polymerase II with the CTD is expressed in the nucleus of eukaryotic cells where it orchestrates the transcription of DNA into mRNA.
Biological function summary

RNA polymerase II CTD repeat YSPTSPS is essential for the transcription progression from initiation to termination. It is part of the large RNA polymerase II complex interacting with various transcription factors and enzymes necessary for RNA processing. The phosphorylation state of the CTD particularly on serine residues regulates interactions with splicing machinery and other RNA processing factors. This modulation ensures the coupling between transcription and RNA processing events controlling mRNA synthesis and maturation.

Pathways

RNA polymerase II CTD repeat YSPTSPS is important in the mRNA synthesis pathway specifically in transcriptional regulation and processing of nascent RNA transcripts. It interacts with proteins such as the transcription factors TFIIH and TFIIB which aid in promoter recognition and open complex formation. The CTD's dynamic phosphorylation pattern allows integration into multiple cellular pathways most importantly connecting transcription with RNA splicing and transport pathways.

Abnormal function or mutations in RNA polymerase II CTD repeat YSPTSPS associate with diseases such as transcription-related syndromes and certain cancers. Deficient CTD phosphorylation can lead to improper mRNA processing resulting in neural developmental disorders. Additionally its interaction with proteins like CDK7 which phosphorylates the CTD links it to tumors where transcriptional dysregulation is a hallmark. Understanding the CTD's role in these diseases provides insight into therapeutic targets and strategies for intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates (By similarity) (PubMed : 23748380, PubMed : 27193682, PubMed : 30190596, PubMed : 9852112). Pol II-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol II pre-initiation complex (PIC) is recruited to DNA promoters, with focused-type promoters containing either the initiator (Inr) element, or the TATA-box found in cell-type specific genes and dispersed-type promoters that often contain hypomethylated CpG islands usually found in housekeeping genes. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA (By similarity) (PubMed : 23748380, PubMed : 27193682, PubMed : 28108474, PubMed : 30190596, PubMed : 9852112). Forms Pol II active center together with the second largest subunit POLR2B/RPB2. Appends one nucleotide at a time to the 3' end of the nascent RNA, with POLR2A/RPB1 most likely contributing a Mg(2+)-coordinating DxDGD motif, and POLR2B/RPB2 participating in the coordination of a second Mg(2+) ion and providing lysine residues believed to facilitate Watson-Crick base pairing between the incoming nucleotide and template base. Typically, Mg(2+) ions direct a 5' nucleoside triphosphate to form a phosphodiester bond with the 3' hydroxyl of the preceding nucleotide of the nascent RNA, with the elimination of pyrophosphate. The reversible pyrophosphorolysis can occur at high pyrophosphate concentrations (By similarity) (PubMed : 30190596, PubMed : 8381534, PubMed : 9852112). Can proofread the nascent RNA transcript by means of a 3' -> 5' exonuclease activity. If a ribonucleotide is mis-incorporated, backtracks along the template DNA and cleaves the phosphodiester bond releasing the mis-incorporated 5'-ribonucleotide (By similarity) (PubMed : 8381534). Through its unique C-terminal domain (CTD, 52 heptapeptide tandem repeats) serves as a platform for assembly of factors that regulate transcription initiation, elongation and termination. CTD phosphorylation on Ser-5 mediates Pol II promoter escape, whereas phosphorylation on Ser-2 is required for Pol II pause release during transcription elongation and further pre-mRNA processing. Additionally, the regulation of gene expression levels depends on the balance between methylation and acetylation levels of the CTD-lysines. Initiation or early elongation steps of transcription of growth-factor-induced immediate early genes are regulated by the acetylation status of the CTD. Methylation and dimethylation have a repressive effect on target genes expression. Cooperates with mRNA splicing machinery in co-transcriptional 5'-end capping and co-transcriptional splicing of pre-mRNA (By similarity) (PubMed : 24207025, PubMed : 26124092).. RNA-dependent RNA polymerase that catalyzes the extension of a non-coding RNA (ncRNA) at the 3'-end using the four ribonucleoside triphosphates as substrates. An internal ncRNA sequence near the 3'-end serves as a template in a single-round Pol II-mediated RNA polymerization reaction. May decrease the stability of ncRNAs that repress Pol II-mediated gene transcription.. (Microbial infection) Acts as an RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicase and transcriptase for the viral RNA circular genome.
See full target information POLR2A phospho S5
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Publications (497)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8712 PubMed41027885

2025

RNA-mediated condensation of TFE3 oncofusions facilitates transcriptional hub formation to promote translocation renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Guo,Rongjie Zhao,Yi-Tsang Lee,Junhua Huang,James Wengler,Logan Rivera,Tingting Hong,Tianlu Wang,Kunjal Rathod,Ashley Suris,Yitian Wu,Xiaoli Cai,Rui Wang,Yubin Zhou,Yun Huang

The Journal of biological chemistry 301:110612 PubMed40840622

2025

Argonaute 1 contributes to the transcriptional silencing of HIV-1.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Goudey,Melissa Ait Said,Juliette Desforges,Sébastien Marie,Marina Morel,Clarisse Berlioz-Torrent,Florence Margottin-Goguet,Stéphane Emiliani,Roy Matkovic,Sarah Gallois-Montbrun

Frontiers in cellular and infection microbiology 15:1548038 PubMed40636257

2025

Aryl hydrocarbon receptor facilitates HSV-1 lytic infection by enhancing viral gene transcription and receptor expression.

Applications

Unspecified application

Species

Unspecified reactive species

Pu Huang,Xiaohong Pan,Hongli Chen,Mengyue Lei,Ying Ma,Xiaomei Guo,Jiaxin Xie,Jixiong Li,Jing Sun,Yunzhang Hu,Jiandong Shi

iScience 28:112778 PubMed40585357

2025

Targeting CDK7/12/13 functional synergism reverses myofibroblast activation and ameliorates lung fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hsiao-Yen Ma,Zhiyu Huang,Surinder Jeet,Claire Emerson,Hannah Bender,Qihao Ren,Ning Ding

Nature communications 16:5313 PubMed40537528

2025

Herpes simplex virus type 1 reshapes host chromatin architecture via transcription machinery hijacking.

Applications

Unspecified application

Species

Unspecified reactive species

Esther González-Almela,Alvaro Castells-Garcia,François Le Dily,Manuel Fernández Merino,Davide Carnevali,Pol Cusco,Luciano Di Croce,Maria Pia Cosma

Nature communications 16:4929 PubMed40436847

2025

RNF20 links the DNA damage response and metabolic rewiring in lung cancer through HIF1α.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Liu,Yongqin Tang,Anshu Singh,Joaquim Vong,Julio Cordero,Arthur Mathes,Rui Gao,Yanhan Jia,Boyan K Garvalov,Till Acker,Gernot Poschet,Rüdiger Hell,Marc A Schneider,Joerg Heineke,Thomas Wieland,Guillermo Barreto,Adelheid Cerwenka,Michael Potente,Sofia-Iris Bibli,Rajkumar Savai,Gergana Dobreva

Nature communications 16:4408 PubMed40355503

2025

Single amino acid mutations in histone H3.3 illuminate the functional significance of H3K4 methylation in plants.

Applications

Unspecified application

Species

Unspecified reactive species

Mande Xue,Lijun Ma,Xiaoyi Li,Huairen Zhang,Fengyue Zhao,Qian Liu,Danhua Jiang

Nucleic acids research 53: PubMed40207626

2025

A two-step regulatory mechanism dynamically controls histone H3 acetylation by SAGA complex at growth-related promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Sevil Zencir,Daniel Dilg,Maria Jessica Bruzzone,Françoise Stutz,Julien Soudet,David Shore,Benjamin Albert

Science advances 11:eadq2519 PubMed40203113

2025

Alpha-synuclein regulates nucleolar DNA double-strand break repair in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Moriah R Arnold,Gabriel M Cohn,Kezia Catharina Oxe,Somarr N Elliott,Cynthia Moore,Allison May Zhou,Peter V Laraia,Sahar Shekoohi,Dillon Brownell,Rosalie C Sears,Randall L Woltjer,Charles K Meshul,Stephan N Witt,Dorthe H Larsen,Vivek K Unni

Cell death & disease 16:124 PubMed39987248

2025

NF-κB-mediated EAAT3 upregulation in antioxidant defense and ferroptosis sensitivity in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Donghua Wen,Wenjing Li,Xiang Song,Min Hu,Yueling Liao,Dongliang Xu,Jiong Deng,Wenzheng Guo
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