AB229488

Anti-RNA polymerase II RPB4 antibody

Name: Anti-RNA polymerase II RPB4 antibody (ab229488) | Abcam
Brand: Abcam Limited
SKU: AB229488
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal RNA polymerase II RPB4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POLR2D.

View Alternative Names

DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RPB16, POLR2D

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II RPB4 antibody (AB229488)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II RPB4 antibody (AB229488)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for RNA polymerase II RPB4 (green) using ab229488 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde/PBS for 15 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

Supplier Data

Western blot - Anti-RNA polymerase II RPB4 antibody (AB229488)

15% SDS-PAGE

All lanes:

Western blot - Anti-RNA polymerase II RPB4 antibody (ab229488) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 16 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human POLR2D. The exact immunogen used to generate this antibody is proprietary information.

O15514

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNA polymerase II RPB4 also known by the alternate name POLR2D is a subunit of the larger RNA polymerase II enzyme complex. This enzyme plays a critical role in catalyzing the transcription of DNA into messenger RNA (mRNA) which is fundamental to protein synthesis. RPB4 is part of the core enzyme contributing to its stability and function. It has a molecular mass of approximately 25 kDa. This protein is widely expressed in eukaryotic cells particularly in the nucleus where transcription occurs.

Biological function summary

RNA polymerase II RPB4 functions as a component of the larger RNA polymerase II complex. It contributes to the regulation and initiation of mRNA synthesis. This subunit has essential roles in transcriptional regulation under stress conditions by stabilizing the RNA polymerase II complex. The interaction with co-factors and other transcription machinery highlights RPB4's involvement in adaptive responses of the cell.

Pathways

RNA polymerase II RPB4 integrates into essential transcription pathways such as the mRNA processing and the transcription initiation pathway. It cooperates with other subunits and factors like TFIIB and TFIIH to effectively facilitate the transcription process. These pathways determine the accurate transcription and downstream processes necessary for gene expression playing an interconnected role with other proteins like TAFs within the transcriptional machinery.

RNA polymerase II RPB4 is associated with several types of cancers as transcription regulation often becomes dysregulated in cancer cells. Disruptions in RPB4 expression or function can also relate to neurodegenerative disorders where transcriptional errors contribute to the disease pathology. Interaction of RPB4 with proteins like p53 may highlight its involvement in these conditions as p53 is a critical regulator of cell cycle and apoptosis often altered in cancerous states.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. POLR2D/RPB4 is part of a subcomplex with POLR2G/RPB7 that binds to a pocket formed by POLR2A/RPB1, POLR2B/RPB2 and POLR2F/RPABC2 at the base of the clamp element. The POLR2D/RPB4-POLR2G/RPB7 subcomplex seems to lock the clamp via POLR2G/RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The POLR2D/RPB4-POLR2G/RPB7 subcomplex binds single-stranded DNA and RNA.

See full target information POLR2D

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

RNA biology 22:1-17 PubMed40590376

2025

SARS-CoV-2 RNA-binding protein suppresses extracellular miRNA release.

Applications

Unspecified application

Species

Unspecified reactive species

Hyejin Mun,Chang Hoon Shin,Qingxuan Fei,Andrea Estefania Lopez Giraldo,Kyoung-Min Choi,Ji Won Lee,Kyungmin Kim,Kyung-Won Min,Leilei Shi,Mark T Bedford,Dong-Chan Kim,Yoo Lim Chun,Seonghyun Ryu,Dongin Kim,Jeong Ho Chang,Ryan T Westrope,Michelle Shay,Edward Nguyen,Junho K Hur,Abigail Agyenda,Nam Chul Kim,Sung-Ung Kang,Woonghee Lee,Je-Hyun Yoon

Heliyon 10:e23167 PubMed38169774

2024

Inhibition of TAF1B impairs ribosome biosynthesis and suppresses cell proliferation in stomach adenocarcinoma through promoting c-MYC mRNA degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Hang-Fei Chen,Zhang-Ping Li,Qi Wu,Chun Yu,Jing-Yi Yan,Yong-Feng Bai,Sheng-Mei Zhu,Mao-Xiang Qian,Ming Liu,Li-Feng Xu,Zheng Peng,Feng Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RNA polymerase II RPB4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-RNA polymerase II RPB4 antibody (AB229488)

Western blot - Anti-RNA polymerase II RPB4 antibody (AB229488)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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