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AB251060

Anti-RNase L antibody [EPR15894] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal RNase L antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RNS4, RNASEL, 2-5A-dependent ribonuclease, 2-5A-dependent RNase, Ribonuclease 4, Ribonuclease L, RNase L

1 Images
Western blot - Anti-RNase L antibody [EPR15894] - BSA and Azide free (AB251060)
  • WB

Supplier Data

Western blot - Anti-RNase L antibody [EPR15894] - BSA and Azide free (AB251060)

This data was developed using ab191392, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RNase L antibody [EPR15894] (<a href='/en-us/products/primary-antibodies/rnase-l-antibody-epr15894-ab191392'>ab191392</a>) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

Y79 cell lysate at 20 µg

Lane 3:

THP1 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 84 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15894

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab251060 is the carrier-free version of ab191392.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis (PubMed : 26263979). Might play a central role in the regulation of mRNA turnover (PubMed : 11585831). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.
See full target information RNASEL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:8127-8139 PubMed34350711

2021

Overexpression of let-7d explains down-regulated KDM3A and ENO2 in the pathogenesis of preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xu,Yonghui Song,Lili Lu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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