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AB317202

Anti-RNase L antibody [HL2341]

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Rabbit Monoclonal RNase L antibody. Suitable for WB and reacts with Human, Mouse, Rat, Dog samples. Immunogen corresponding to Recombinant Fragment Protein within Human RNASEL.

View Alternative Names

RNS4, RNASEL, 2-5A-dependent ribonuclease, 2-5A-dependent RNase, Ribonuclease 4, Ribonuclease L, RNase L

5 Images
Western blot - Anti-RNase L antibody [HL2341] (AB317202)
  • WB

Supplier Data

Western blot - Anti-RNase L antibody [HL2341] (AB317202)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317202 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RNase L antibody [HL2341] (ab317202) at 1/1000 dilution

Lane 1:

K562 whole cell extracts at 30 µg

Lane 2:

THP-1 whole cell extracts at 30 µg

Lane 3:

HL-60 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-RNase L antibody [HL2341] (AB317202)
  • WB

Supplier Data

Western blot - Anti-RNase L antibody [HL2341] (AB317202)

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317202 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RNase L antibody [HL2341] (ab317202) at 1/1000 dilution

Lane 1:

TT whole cell extracts at 30 µg

Lane 2:

U266B1 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-RNase L antibody [HL2341] (AB317202)
  • WB

Supplier Data

Western blot - Anti-RNase L antibody [HL2341] (AB317202)

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE and the membrane was blotted with ab317202 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RNase L antibody [HL2341] (ab317202) at 1/1000 dilution

All lanes:

C6 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-RNase L antibody [HL2341] (AB317202)
  • WB

Supplier Data

Western blot - Anti-RNase L antibody [HL2341] (AB317202)

Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE and the membrane was blotted with ab317202 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RNase L antibody [HL2341] (ab317202) at 1/1000 dilution

All lanes:

Mouse colon tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-RNase L antibody [HL2341] (AB317202)
  • WB

Supplier Data

Western blot - Anti-RNase L antibody [HL2341] (AB317202)

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE and the membrane was blotted with ab317202 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-RNase L antibody [HL2341] (ab317202) at 1/1000 dilution

All lanes:

MDCK whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2341

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat, Dog

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RNASEL.

Q05823

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis (PubMed : 26263979). Might play a central role in the regulation of mRNA turnover (PubMed : 11585831). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.
See full target information RNASEL
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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