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AB200717

Anti-RNASE4 antibody

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(5 Publications)

Rabbit Polyclonal RNASE4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RNASE4 aa 50-100.

View Alternative Names

RNS4, RNASE4, Ribonuclease 4, RNase 4

1 Images
Western blot - Anti-RNASE4 antibody (AB200717)
  • WB

Supplier Data

Western blot - Anti-RNASE4 antibody (AB200717)

All lanes:

Western blot - Anti-RNASE4 antibody (ab200717) at 1/500 dilution

Lane 1:

HEK293T whole cell lysate

Lane 2:

sp2/0 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human RNASE4 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P34096

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab200717 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Storage buffer
pH: 7.2 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ribonuclease 4 also known as RNase 4 is a member of the ribonuclease A superfamily. This protein has an approximate mass of 16 kDa. RNase 4 functions as an endonuclease catalyzing the cleavage of RNA on the 3' side of pyrimidines like uridine and cytidine. It shows high expression levels in the pancreas and liver and is also found in other tissues such as the spleen and heart.
Biological function summary

RNase 4 participates in the maintenance of extracellular RNA equilibrium which is important for overall cellular health. It does not typically form complexes like some other ribonucleases do. It helps control RNA turnover by degrading extracellular RNA preventing the accumulation of potentially harmful nucleic acids in the extracellular environment. This activity supports other cellular functions by modulating levels of specific RNA molecules.

Pathways

RNase 4 plays a role in the regulation of nucleic acid metabolism pathways. It works alongside other enzymes such as RNase A to manage RNA turnover which is a critical aspect of gene expression regulation and cellular homeostasis. By influencing these pathways RNase 4 indirectly affects processes involved in the management of cellular stress responses and apoptotic pathways.

RNase 4 presents potential implications in cancer and inflammatory diseases. Disruption of its normal activity might contribute to abnormal RNA processing and turnover which can manifest in these conditions. Connections to proteins like RNase A and other related nucleic acid-processing enzymes highlight its role in maintaining cellular function and preventing pathological states through effective RNA management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cleaves preferentially after uridine bases (PubMed : 3467790). Has antimicrobial activity against uropathogenic E.coli (UPEC) (PubMed : 33818125). Probably contributes to urinary tract sterility (PubMed : 33818125).
See full target information RNASE4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:5796 PubMed37723159

2023

Discovery of the major 15-30 nt mammalian small RNAs, their biogenesis and function.

Applications

Unspecified application

Species

Unspecified reactive species

Hejin Lai,Ning Feng,Qiwei Zhai

Proceedings of the National Academy of Sciences of the United States of America 120:e2213363120 PubMed36652479

2023

Repurposing HDAC inhibitors to enhance ribonuclease 4 and 7 expression and reduce urinary tract infection.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Schwartz,M Skye Bochter,Aaron Simoni,Kristin Bender,Juan de Dios Ruiz Rosado,Israel Cotzomi-Ortega,Yuriko I Sanchez-Zamora,Brian Becknell,Sarah Linn,Birong Li,Nicholas Santoro,Tad Eichler,John David Spencer

American journal of physiology. Renal physiology 320:F972-F983 PubMed33818125

2021

Expression and function of human ribonuclease 4 in the kidney and urinary tract.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Bender,Laura L Schwartz,Ariel Cohen,Claudia Mosquera Vasquez,Matthew J Murtha,Tad Eichler,Jason P Thomas,Ashley Jackson,John David Spencer

The Journal of biological chemistry 294:16930-16941 PubMed31582561

2019

Angiogenin generates specific stress-induced tRNA halves and is not involved in tRF-3-mediated gene silencing.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangli Su,Canan Kuscu,Asrar Malik,Etsuko Shibata,Anindya Dutta

The Journal of clinical investigation 128:5634-5646 PubMed30418175

2018

Insulin receptor signaling regulates renal collecting duct and intercalated cell antibacterial defenses.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Murtha,Tad Eichler,Kristin Bender,Jackie Metheny,Birong Li,Andrew L Schwaderer,Claudia Mosquera,Cindy James,Laura Schwartz,Brian Becknell,John David Spencer
View all publications
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Product promise

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