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AB104159

Anti-RNF10 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal RNF10 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RNF10 aa 100-350.

View Alternative Names

KIAA0262, RIE2, RNF10, E3 ubiquitin-protein ligase RNF10, RING finger protein 10

2 Images
Western blot - Anti-RNF10 antibody (AB104159)
  • WB

Unknown

Western blot - Anti-RNF10 antibody (AB104159)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RNF10 antibody (ab104159) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 90 kDa

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Western blot - Anti-RNF10 antibody (AB104159)
  • WB

Supplier Data

Western blot - Anti-RNF10 antibody (AB104159)

5% SDS PAGE.

All lanes:

Western blot - Anti-RNF10 antibody (ab104159) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 90 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human RNF10 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

Q8N5U6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF10 also known as RING finger protein 10 is an E3 ubiquitin-protein ligase that plays a role in attaching ubiquitin molecules to target substrates marking them for proteasomal degradation. RNF10 has a molecular weight of approximately 75 kDa. It is expressed in various tissues including the brain heart and skeletal muscle. RNF10 contains a RING finger domain important for its ligase activity allowing it to interact with E2 ubiquitin-conjugating enzymes.
Biological function summary

RNF10 participates in regulating cellular processes like neurodevelopment and muscle differentiation. It interacts with other proteins to form complexes that influence cell signaling pathways and transcription regulation. This protein's activities affect neuronal growth and synaptic plasticity important for learning and memory. RNF10 also has implications in muscle cells where it modulates the expression of genes related to muscle function.

Pathways

RNF10 takes part in the MAPK signaling pathway which plays a role in cell proliferation and differentiation. In this pathway RNF10 interacts with proteins such as MEK and ERK allowing it to influence downstream signaling processes. Additionally RNF10 has connections to the Wnt signaling pathway impacting gene expression patterns important for development. These interactions highlight its role in orchestrating complex cellular responses.

RNF10 is linked to neurological and muscular conditions. Its involvement in neurodevelopment suggests a connection to neurodegenerative diseases like Alzheimer's where protein degradation processes may go awry. In muscular disorders RNF10 may interact with proteins like MYOD1 influencing muscle differentiation and regeneration. These associations highlight the importance of understanding RNF10's role in healthy and diseased states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that catalyzes monoubiquitination of 40S ribosomal proteins RPS2/us5 and RPS3/us3 in response to ribosome stalling (PubMed : 34348161, PubMed : 34469731). Part of a ribosome quality control that takes place when ribosomes have stalled during translation initiation (iRQC) : RNF10 acts by mediating monoubiquitination of RPS2/us5 and RPS3/us3, promoting their degradation by the proteasome (PubMed : 34348161, PubMed : 34469731). Also promotes ubiquitination of 40S ribosomal proteins in response to ribosome stalling during translation elongation (PubMed : 34348161). The action of RNF10 in iRQC is counteracted by USP10 (PubMed : 34469731). May also act as a transcriptional factor involved in the regulation of MAG (Myelin-associated glycoprotein) expression (By similarity). Acts as a regulator of Schwann cell differentiation and myelination (By similarity).
See full target information RNF10
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of dairy science 107:3127-3139 PubMed37939835

2023

Inositol-requiring enzyme 1α and c-Jun N-terminal kinase axis activation contributes to intracellular lipid accumulation in calf hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Wenwen Gao,Yanxi Wang,Siyu Liu,Guojin Li,Qi Shao,Cai Zhang,Liguang Cao,Kai Liu,Wenrui Gao,Zifeng Yang,Yifei Dong,Xiliang Du,Lin Lei,Guowen Liu,Xinwei Li

FEBS open bio 11:386-394 PubMed33249776

2021

Reduced RING finger protein 10 expression in macrophages is associated with aging-related inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyuan Cao,Lidan Liu,Yueyi Zhang,Yingyun Yang
View all publications
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