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AB97303

Anti-RNF114 antibody

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(3 Publications)

Rabbit Polyclonal RNF114 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RNF114 aa 1 to C-terminus.

View Alternative Names

ZNF228, ZNF313, RNF114, E3 ubiquitin-protein ligase RNF114, RING finger protein 114, RING-type E3 ubiquitin transferase RNF114, Zinc finger protein 228, Zinc finger protein 313

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-RNF114 antibody (AB97303)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RNF114 antibody (AB97303)

Images show immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using 1/200 ab97303 alone (1) and merged with DNA probe (2).

Western blot - Anti-RNF114 antibody (AB97303)
  • WB

Unknown

Western blot - Anti-RNF114 antibody (AB97303)

12 % SDS PAGE

All lanes:

Western blot - Anti-RNF114 antibody (ab97303) at 1/1000 dilution

All lanes:

HepG2 whole cell lyate at 30 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RNF114 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y508

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF114 also known as zinc finger protein 313 is a RING-type E3 ubiquitin ligase with a mass of approximately 51 kDa. It plays a mechanical role in tagging proteins for ubiquitination marking them for degradation by the proteasome. RNF114 is expressed mainly in the skin but can also be found in immune cells such as T-cells and macrophages. Its E3 ubiquitin ligase activity is important for regulating protein stability and cellular processes.
Biological function summary

RNF114 has a significant role in regulating immune response and cell proliferation. It participates in pathways that control inflammation by modulating the activity of NF-kB an important transcription factor in immune response. RNF114 also influences cell cycle regulation through its interactions with other proteins. While it does not form a strict complex it interacts closely with substrates it ubiquitinates to affect signaling pathways critical for cellular responses.

Pathways

Multiple cellular processes engage RNF114. It plays a part in the NF-kB signaling pathway and the Wnt signaling pathway. In the NF-kB signaling pathway RNF114 helps control the stability of specific proteins maintaining a balance in immune responses. In the Wnt pathway it has been indicated to interact with proteins like β-catenin influencing gene expression that governs cell growth and differentiation both important in development and immune function.

A few studies connect RNF114 with autoimmune diseases and cancer. For example its dysregulation has associations with psoriasis an immune-mediated disease leading to excessive skin cell proliferation. Dysregulation of RNF114 has also been noted in certain cancer types possibly through interactions with proteins such as tumor suppressors affecting pathways regulating cell growth and death. Understanding these interactions can provide insights into therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that promotes the ubiquitination of various substrates (PubMed : 23645206, PubMed : 25165885). In turn, participates in the regulation of many biological processes including cell cycle, apoptosis, osteoclastogenesis as well as innate or adaptive immunity (PubMed : 25165885, PubMed : 28708287). Acts as a negative regulator of NF-kappa-B-dependent transcription by promoting the ubiquitination and stabilization of the NF-kappa-B inhibitor TNFAIP3 (PubMed : 25165885). May promote the ubiquitination of TRAF6 as well (PubMed : 28708287). Acts also as a negative regulator of T-cell activation (PubMed : 25165885). Inhibits cellular dsRNA responses and interferon production by targeting MAVS component for proteasomal degradation (PubMed : 25165885). Ubiquitinates the CDK inhibitor CDKN1A leading to its degradationand probably also CDKN1B and CDKN1C (PubMed : 23645206). This activity stimulates cell cycle G1-to-S phase transition and suppresses cellular senescence. May play a role in spermatogenesis.
See full target information RNF114
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 19:134 PubMed38342889

2024

MicroRNA-877-5p promotes osteoblast differentiation by targeting EIF4G2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

YingChao Shen,Yang Zhang,Qiang Wang,Bo Jiang,XiaoWei Jiang,Bin Luo

Nature communications 13:5493 PubMed36123338

2022

Extracellular fibrinogen-binding protein released by intracellular Staphylococcus aureus suppresses host immunity by targeting TRAF3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokai Zhang,Tingrong Xiong,Lin Gao,Yu Wang,Luxuan Liu,Tian Tian,Yun Shi,Jinyong Zhang,Zhuo Zhao,Dongshui Lu,Ping Luo,Weijun Zhang,Ping Cheng,Haiming Jing,Qiang Gou,Hao Zeng,Dapeng Yan,Quanming Zou

Veterinary microbiology 246:108746 PubMed32605740

2020

Proteasomal degradation of nonstructural protein 12 by RNF114 suppresses porcine reproductive and respiratory syndrome virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanzhe Bai,Liwei Li,Tongling Shan,Yujiao Zhang,Xiaoyong Chen,Fei Gao,Yifeng Jiang,Yanjun Zhou,Guoxin Li,Lingxue Yu,Ning Kong,Zhiyong Ma,Guangzhi Tong
View all publications
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