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Rabbit Recombinant Monoclonal RNF115 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-RNF115 antibody [EPR14539] - BSA and Azide free (AB250926), expandable thumbnail
  • Western blot - Anti-RNF115 antibody [EPR14539] - BSA and Azide free (AB250926), expandable thumbnail
  • Western blot - Anti-RNF115 antibody [EPR14539] - BSA and Azide free (AB250926), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

E3 ubiquitin-protein ligase that catalyzes the 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of various substrates and thereby plays a role in a number of signaling pathways including autophagy, innate immunity, cell proliferation and cell death (PubMed:20019814, PubMed:30689267). Plays a role in the endosomal trafficking and degradation of membrane receptors including EGFR, FLT3, MET and CXCR4 through their polyubiquitination. Participates together with BST2 in antiviral immunity by facilitating the internalization of HIV-1 virions into intracellular vesicles leading to their lysosomal degradation (PubMed:20019814). Possesses also an antiviral activity independently of BST2 by promoting retroviral GAG proteins ubiquitination, redistribution to endo-lysosomal compartments and, ultimately, lysosomal degradation (PubMed:24852021). Catalyzes distinct types of ubiquitination on MAVS and STING1 at different phases of viral infection to promote innate antiviral response (PubMed:33139700). Mediates the 'Lys-48'-linked ubiquitination of MAVS leading to its proteasomal degradation and ubiquitinates STING1 via 'Lys-63'-linked polyubiquitination, critical for its oligomerization and the subsequent recruitment of TBK1 (PubMed:33139700). Plays a positive role in the autophagosome-lysosome fusion by interacting with STX17 and enhancing its stability without affecting 'Lys-48'- or 'Lys-63'-linked polyubiquitination levels, which in turn promotes autophagosome maturation (PubMed:32980859). Negatively regulates TLR-induced expression of proinflammatory cytokines by catalyzing 'Lys-11'-linked ubiquitination of RAB1A and RAB13 to inhibit post-ER trafficking of TLRs to the Golgi by RAB1A and subsequently from the Golgi apparatus to the cell surface by RAB13 (PubMed:35343654).

Alternative names

ZNF364, RNF115, E3 ubiquitin-protein ligase RNF115, RING finger protein 115, RING-type E3 ubiquitin transferase RNF115, Rab7-interacting RING finger protein, Zinc finger protein 364, Rabring 7

Recommended products

Rabbit Recombinant Monoclonal RNF115 antibody. Carrier free. Suitable for WB and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14539
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250926 is the carrier-free version of Anti-RNF115 antibody [EPR14539] ab187642.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RNF115 also known as RING Finger Protein 115 or BRE1B is an E3 ubiquitin-protein ligase. This protein weighs approximately 34 kDa and belongs to the RBR (RING1-IBR-RING2) E3 ubiquitin ligase family. RNF115 functions mechanically by attaching ubiquitin molecules to specific protein substrates marking them for proteasomal degradation. RNF115 is found in various tissues showing higher expression in the brain and reproductive organs. It plays a mechanical role in proteostasis by facilitating the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to target proteins.

Biological function summary

RNF115 affects protein regulation through its involvement in ubiquitin-mediated processes. It interacts with different substrates and has been identified as part of the BRCA1-A complex which is important in DNA repair mechanisms. It helps regulate cellular levels and stability of proteins impacting cell cycle progression and response to DNA damage. Its activity ensures proper cellular development and homeostasis by maintaining protein turnover.

Pathways

RNF115 engages in the ubiquitin-proteasome system and is implicated in the DNA damage response pathways. It acts within the ubiquitination pathways collaborating with other proteins like BRCA1 and UBE2D1 to influence protein degradation and DNA repair processes. RNF115's role in the regulation of the DNA damage response highlights its connection to cellular stress resilience linking it to cellular pathways that maintain genetic stability.

Associated diseases and disorders

Alterations in RNF115 expression or function can link to cancer development and neurodegenerative diseases. Misregulation can impact pathways involving BRCA1 associated with breast cancer contributing to abnormal cell proliferation and genomic instability. Additionally disruptions in its protein degradation function may relate to neurodegenerative diseases where abnormal protein accumulation occurs such as Alzheimer's disease. The connection of RNF115 with proteins like BRCA1 and UBE2D1 in these conditions highlights its importance in maintaining cellular health and preventing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-RNF115 antibody [EPR14539] - BSA and Azide free (ab250926), expandable thumbnail

    Western blot - Anti-RNF115 antibody [EPR14539] - BSA and Azide free (ab250926)

    This data was developed using Anti-RNF115 antibody [EPR14539] ab187642, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RNF115 antibody [EPR14539] (Anti-RNF115 antibody [EPR14539] ab187642) at 1/1000 dilution

    Lane 1: RAW 264.7 cell lysate at 10 µg

    Lane 2: PC-12 cell lysate at 10 µg

    Lane 3: NIH/3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

  • Western blot - Anti-RNF115 antibody [EPR14539] - BSA and Azide free (ab250926), expandable thumbnail

    Western blot - Anti-RNF115 antibody [EPR14539] - BSA and Azide free (ab250926)

    This data was developed using Anti-RNF115 antibody [EPR14539] ab187642, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RNF115 antibody [EPR14539] (Anti-RNF115 antibody [EPR14539] ab187642) at 1/2000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: HeLa lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

  • Western blot - Anti-RNF115 antibody [EPR14539] - BSA and Azide free (ab250926), expandable thumbnail

    Western blot - Anti-RNF115 antibody [EPR14539] - BSA and Azide free (ab250926)

    This data was developed using Anti-RNF115 antibody [EPR14539] ab187642, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-RNF115 antibody [EPR14539] (Anti-RNF115 antibody [EPR14539] ab187642) at 1/1000 dilution

    All lanes: PC-3 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 34 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com