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AB211450

Anti-RNF125 antibody

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(3 Publications)

Rabbit Polyclonal RNF125 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human RNF125 aa 100-200.

View Alternative Names

E3 ubiquitin-protein ligase RNF125, RING finger protein 125, T-cell RING activation protein 1, TRAC-1, RNF125

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF125 antibody (AB211450)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF125 antibody (AB211450)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human small intestine tissue labeling RNF125 with ab211450 at 5ug/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF125 antibody (AB211450)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF125 antibody (AB211450)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human thymus tissue labeling RNF125 with ab211450 at 5μg/mL.

Western blot - Anti-RNF125 antibody (AB211450)
  • WB

Supplier Data

Western blot - Anti-RNF125 antibody (AB211450)

All lanes:

Western blot - Anti-RNF125 antibody (ab211450) at 1/500 dilution

Lane 1:

K562 cell extract

Lane 2:

HT29 cell extract

Lane 3:

K562 cell extract with synthesized RNF125 peptide

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human RNF125 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.

Q96EQ8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF125 also known as TRAC-1 is a protein encoded by the RNF125 gene. It belongs to the RING finger family and functions as an E3 ubiquitin-protein ligase which attaches ubiquitin to protein substrates directing them for degradation. RNF125 has a molecular mass of approximately 51 kDa. Researchers observe its expression predominantly in immune-related tissues such as the thymus spleen and peripheral blood leukocytes.
Biological function summary

RNF125 participates in the immune response by modulating the activity and stability of key signaling proteins. It forms part of a regulatory complex that influences immune signaling pathways. RNF125 acts by ubiquitinating proteins like MAVS which play a role in antiviral responses. Through ubiquitination RNF125 helps regulate the balance between activation and suppression of immune reactions ensuring proper immune function.

Pathways

Researchers have identified RNF125 as an important component of the type I interferon signaling pathway and the TLR (Toll-like receptor) pathway. In the type I interferon pathway RNF125 targets proteins such as MAVS and RIG-I playing a role in antiviral defense. Furthermore RNF125 interacts with TRAF6 in the TLR pathway influencing the body's innate immunity and inflammation responses.

RNF125 has connections to immune-related conditions such as autoimmune diseases and chronic viral infections. For example aberrant regulation of RNF125 activity might contribute to systemic lupus erythematosus (SLE) through improper control of MAVS and other immune signaling proteins. Moreover in the context of chronic viral infections RNF125's role in targeting RIG-I for degradation could hinder the immune system's ability to clear persistent viral infections. Understanding these associations provides insights into potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53 (PubMed : 15843525, PubMed : 17460044, PubMed : 17643463, PubMed : 25591766, PubMed : 26027934, PubMed : 26471729, PubMed : 27411375). Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation (PubMed : 17460044, PubMed : 26471729). Mediates ubiquitination and subsequent degradation of p53/TP53 (PubMed : 25591766). Mediates ubiquitination and subsequent degradation of JAK1 (PubMed : 26027934). Acts as a positive regulator of T-cell activation (PubMed : 15843525).
See full target information RNF125
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Microorganisms 12: PubMed39065185

2024

Exosomal Small RNA Sequencing Profiles in Plasma from Subjects with Latent Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaogang Cui,Hangting Meng,Miao Li,Xia Chen,Dan Yuan,Changxin Wu

The Journal of biological chemistry 297:101190 PubMed34517008

2021

African swine fever virus protein MGF-505-7R promotes virulence and pathogenesis by inhibiting JAK1- and JAK2-mediated signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Jing Zhang,Wenping Yang,Pan Li,Yi Ru,Weifang Kang,LuLu Li,Yong Ran,Haixue Zheng

Cell reports 35:109272 PubMed34161773

2021

Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Shen,Kejun Tang,Dongdong Chen,Mengying Hong,Fangfang Sun,SaiSai Wang,Yuehai Ke,Tingting Wu,Ren Sun,Jing Qian,Yushen Du
View all publications
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