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AB74373

Anti-RNF125 antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal RNF125 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

E3 ubiquitin-protein ligase RNF125, RING finger protein 125, T-cell RING activation protein 1, TRAC-1, RNF125

2 Images
Western blot - Anti-RNF125 antibody (AB74373)
  • WB

AbReview39333****

Western blot - Anti-RNF125 antibody (AB74373)

All lanes:

Western blot - Anti-RNF125 antibody (ab74373) at 1/200 dilution

Lane 1:

293T whole cell lysate at 5 µg

Lane 2:

Mouse ES (129Sv) whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

Predicted band size: 26 kDa

Observed band size: 100 kDa,26 kDa

false

Exposure time: 1min

This image is courtesy of an anonymous Abreview

Western blot - Anti-RNF125 antibody (AB74373)
  • WB

Unknown

Western blot - Anti-RNF125 antibody (AB74373)

All lanes:

Western blot - Anti-RNF125 antibody (ab74373) at 1/500 dilution

Lane 1:

Extracts from K562 cells at 5 µg

Lane 2:

Extracts from HT-29 cells at 5 µg

Lane 3:

Extracts from K562 cells at 5 µg with immunising peptide

Predicted band size: 26 kDa

Observed band size: 100 kDa,23 kDa,26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RNF125 also known as TRAC-1 is a protein encoded by the RNF125 gene. It belongs to the RING finger family and functions as an E3 ubiquitin-protein ligase which attaches ubiquitin to protein substrates directing them for degradation. RNF125 has a molecular mass of approximately 51 kDa. Researchers observe its expression predominantly in immune-related tissues such as the thymus spleen and peripheral blood leukocytes.
Biological function summary

RNF125 participates in the immune response by modulating the activity and stability of key signaling proteins. It forms part of a regulatory complex that influences immune signaling pathways. RNF125 acts by ubiquitinating proteins like MAVS which play a role in antiviral responses. Through ubiquitination RNF125 helps regulate the balance between activation and suppression of immune reactions ensuring proper immune function.

Pathways

Researchers have identified RNF125 as an important component of the type I interferon signaling pathway and the TLR (Toll-like receptor) pathway. In the type I interferon pathway RNF125 targets proteins such as MAVS and RIG-I playing a role in antiviral defense. Furthermore RNF125 interacts with TRAF6 in the TLR pathway influencing the body's innate immunity and inflammation responses.

RNF125 has connections to immune-related conditions such as autoimmune diseases and chronic viral infections. For example aberrant regulation of RNF125 activity might contribute to systemic lupus erythematosus (SLE) through improper control of MAVS and other immune signaling proteins. Moreover in the context of chronic viral infections RNF125's role in targeting RIG-I for degradation could hinder the immune system's ability to clear persistent viral infections. Understanding these associations provides insights into potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins, such as RIGI, MAVS/IPS1, IFIH1/MDA5, JAK1 and p53/TP53 (PubMed : 15843525, PubMed : 17460044, PubMed : 17643463, PubMed : 25591766, PubMed : 26027934, PubMed : 26471729, PubMed : 27411375). Acts as a negative regulator of type I interferon production by mediating ubiquitination of RIGI at 'Lys-181', leading to RIGI degradation (PubMed : 17460044, PubMed : 26471729). Mediates ubiquitination and subsequent degradation of p53/TP53 (PubMed : 25591766). Mediates ubiquitination and subsequent degradation of JAK1 (PubMed : 26027934). Acts as a positive regulator of T-cell activation (PubMed : 15843525).
See full target information RNF125
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 10:926 PubMed30804369

2019

Cellular TRIM33 restrains HIV-1 infection by targeting viral integrase for proteasomal degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Hashim Ali,Miguel Mano,Luca Braga,Asma Naseem,Bruna Marini,Diem My Vu,Chiara Collesi,Germana Meroni,Marina Lusic,Mauro Giacca

Journal of immunology (Baltimore, Md. : 1950) 191:660-9 PubMed23772026

2013

Human bocavirus VP2 upregulates IFN-β pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I.

Applications

Unspecified application

Species

Unspecified reactive species

Huanle Luo,Zhenfeng Zhang,Zhenhua Zheng,Xianliang Ke,Xiaowei Zhang,Qian Li,Yan Liu,Bingke Bai,Panyong Mao,Qinxue Hu,Hanzhong Wang
View all publications
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Product promise

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