Anti-RNF126 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF126 antibody (AB234812)

Immunohistochemical analysis of paraffin-embedded Human pancreatic tissue labeling RNF126 with ab234812 at 1/300 dilution followed by a ready to use Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-RNF126 antibody (AB234812)

Immunofluorescent analysis of HeLa (human cervical adenocarcinoma epithelial cell) cells labelling RNF126 with ab234812 at 1/30 dilution. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF126 antibody (AB234812)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling RNF126 with ab234812 at 1/300 dilution followed by a ready to use Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF126 antibody (AB234812)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RNF126 with ab234812 at 1/300 dilution followed by a ready to use Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

• WB

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Western blot - Anti-RNF126 antibody (AB234812)

All lanes:

Western blot - Anti-RNF126 antibody (ab234812) at 1/5000 dilution

All lanes:

Mouse liver tissue

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

false