Rabbit Polyclonal RNF126 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase RNF126 aa 1-250.
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- Breast cancer research : BCR 24:922022Ring finger protein 126 promotes breast cancer metastasis and serves as a potential target to improve the therapeutic sensitivity of ATR inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou Pan,Yuchao Yang,Rong Huang,Huawei Yang,Qinghua Huang,Yinan Ji,Jingxing Dai,Kun Qiao,Wei Tang,Longgui Xie,Ming Yin,Jun Ouyang,Shipeng Ning,Danke SuPubMed 36539893
- Cells 9:2020Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSun-Yong Kim et. al.PubMed 32131492
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase RNF126 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BV68
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
E3 ubiquitin-protein ligase that mediates ubiquitination oF target proteins (PubMed:23277564, PubMed:24275455, PubMed:24981174, PubMed:36563124). Depending on the associated E2 ligase, mediates 'Lys-27'-, 'Lys-29'-, 'Lys-48'- and/or 'Lys-63'-linked polyubiquitination of substrates (PubMed:36563124). Part of a BAG6-dependent quality control process ensuring that proteins of the secretory pathway that are mislocalized to the cytosol are degraded by the proteasome. Probably acts by providing the ubiquitin ligase activity associated with the BAG6 complex and be responsible for ubiquitination of the hydrophobic mislocalized proteins and their targeting to the proteasome (PubMed:24981174, PubMed:29042515). May also play a role in the endosomal recycling of IGF2R, the cation-independent mannose-6-phosphate receptor (PubMed:24275455). May play a role in the endosomal sorting and degradation of several membrane receptors including EGFR, FLT3, MET and CXCR4, by mediating their ubiquitination (PubMed:23418353). By ubiquitinating CDKN1A/p21 and targeting it for degradation, may also promote cell proliferation (PubMed:23026136). May monoubiquitinate AICDA (PubMed:23277564). Acts as a regulator of DNA repair by mediating 'Lys-27'- and 'Lys-29'-linked polyubiquitination of MRE11, thereby promoting the exonuclease activity of MRE11 (PubMed:36563124).
Alternative names
E3 ubiquitin-protein ligase RNF126, RING finger protein 126, RNF126
Recommended products
Rabbit Polyclonal RNF126 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase RNF126 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase RNF126 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BV68
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RNF126 also known as RING finger protein 126 functions as an E3 ubiquitin ligase. It possesses a molecular mass of approximately 30 kDa and is expressed in various tissues including the brain heart and kidneys. The protein contains a RING finger domain which helps mediate substrate ubiquitination. This post-translational modification marks proteins for degradation by the proteasome regulating protein turnover and function in cells.
- Biological function summary
RNF126 plays significant roles in protein homeostasis and cellular stress responses. It interacts with the p97/VCP complex facilitating the extraction of polyubiquitinated proteins from cellular structures for subsequent degradation. Additionally RNF126 regulates the stability of various substrates involved in cellular processes such as DNA repair and the cell cycle. This regulation ensures proper cell function and adaptation to varying environmental conditions.
- Pathways
RNF126 participates in the ubiquitin-proteasome system which is critical for the degradation of misfolded or damaged proteins. It also takes part in pathways related to DNA damage response where its function overlaps with proteins like BRCA1 and RAD51. The protein's involvement in these pathways highlights its importance in maintaining genomic stability and proper cellular function. RNF126 ensures proteins are appropriately degraded when no longer needed or damaged maintaining cellular integrity.
- Associated diseases and disorders
RNF126 has been linked to cancer and neurodegenerative diseases. In cancer RNF126 is associated with tumor progression and resistance to therapy potentially through its interaction with proteins such as HER2 and p53. In neurodegenerative diseases impairments in RNF126 function could impact protein clearance contributing to the accumulation of toxic protein aggregates. These relationships suggest that RNF126 might serve as a target for therapeutic intervention in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-RNF126 antibody (ab234812)
All lanes: Western blot - Anti-RNF126 antibody (ab234812) at 1/5000 dilution
All lanes: Mouse liver tissue
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF126 antibody (ab234812)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling RNF126 with ab234812 at 1/300 dilution followed by a ready to use Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunocytochemistry/ Immunofluorescence - Anti-RNF126 antibody (ab234812)
Immunofluorescent analysis of HeLa (human cervical adenocarcinoma epithelial cell) cells labelling RNF126 with ab234812 at 1/30 dilution. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L). Counter-stained with DAPI.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF126 antibody (ab234812)
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling RNF126 with ab234812 at 1/300 dilution followed by a ready to use Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RNF126 antibody (ab234812)
Immunohistochemical analysis of paraffin-embedded Human pancreatic tissue labeling RNF126 with ab234812 at 1/300 dilution followed by a ready to use Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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